Efficacy of different carrier gases for barrier discharge plasma generation compared to chlorhexidine on the survival of pseudomonas aeruginosa embedded in biofilm in vitro

Rutger Matthes, Nils Olaf Hübner, C. Bender, I. Koban, S. Horn, S. Bekeschus, K. D. Weltmann, T. Kocher, Axel Kramer, O. Assadian

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Because of its antimicrobial properties, nonthermal plasma could serve as an alternative to chemical antisepsis in wound treatment. Therefore, this study investigated the inactivation of biofilm-embedded Pseudomonas aeruginosa SG81 by a surface barrier-discharged (SBD) plasma for 30, 60, 150 and 300 s. In order to optimize the efficacy of the plasma, different carrier gases (argon, argon admixed with 1% oxygen, and argon with increased humidity up to approx. 80%) were tested and compared against 0.1% chlorhexidine digluconate (CHG) exposure for 600 s. The antimicrobial efficacy was determined by calculating the difference between the numbers of colony-forming units (CFU) of treated and untreated biofilms. Living bacteria were distinguished from dead by fluorescent staining and confocal laser scanning microscopy. Both SBD plasmas and CHG showed significant antimicrobial effects compared to the untreated control. However, plasma treatment led to a higher antimicrobial reduction (argon plasma 4.9 log10 CFU/cm2, argon with admixed oxygen 3 log10 CFU/cm2, and with increased gas humidity 2.7 log10 CFU/cm2 after 300 s) compared to CHG. In conclusion, SBD plasma is suitable as an alternative to CHG for inactivation of Pseudomonas aeruginosa embedded in biofilm. Further development of SBD plasma sources and research on the role of carrier gases and humidity may allow their clinical application for wound management in the future.

Original languageEnglish
Pages (from-to)148-157
Number of pages10
JournalSkin Pharmacology and Physiology
Volume27
Issue number3
DOIs
Publication statusPublished - Feb 2014
Externally publishedYes

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Chlorhexidine
Biofilms
Pseudomonas aeruginosa
Gases
Argon
Stem Cells
Humidity
Antisepsis
Oxygen
In Vitro Techniques
Wounds and Injuries
Confocal Microscopy
Staining and Labeling
Bacteria
chlorhexidine gluconate
Research

Cite this

Matthes, Rutger ; Hübner, Nils Olaf ; Bender, C. ; Koban, I. ; Horn, S. ; Bekeschus, S. ; Weltmann, K. D. ; Kocher, T. ; Kramer, Axel ; Assadian, O. / Efficacy of different carrier gases for barrier discharge plasma generation compared to chlorhexidine on the survival of pseudomonas aeruginosa embedded in biofilm in vitro. In: Skin Pharmacology and Physiology. 2014 ; Vol. 27, No. 3. pp. 148-157.
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abstract = "Because of its antimicrobial properties, nonthermal plasma could serve as an alternative to chemical antisepsis in wound treatment. Therefore, this study investigated the inactivation of biofilm-embedded Pseudomonas aeruginosa SG81 by a surface barrier-discharged (SBD) plasma for 30, 60, 150 and 300 s. In order to optimize the efficacy of the plasma, different carrier gases (argon, argon admixed with 1{\%} oxygen, and argon with increased humidity up to approx. 80{\%}) were tested and compared against 0.1{\%} chlorhexidine digluconate (CHG) exposure for 600 s. The antimicrobial efficacy was determined by calculating the difference between the numbers of colony-forming units (CFU) of treated and untreated biofilms. Living bacteria were distinguished from dead by fluorescent staining and confocal laser scanning microscopy. Both SBD plasmas and CHG showed significant antimicrobial effects compared to the untreated control. However, plasma treatment led to a higher antimicrobial reduction (argon plasma 4.9 log10 CFU/cm2, argon with admixed oxygen 3 log10 CFU/cm2, and with increased gas humidity 2.7 log10 CFU/cm2 after 300 s) compared to CHG. In conclusion, SBD plasma is suitable as an alternative to CHG for inactivation of Pseudomonas aeruginosa embedded in biofilm. Further development of SBD plasma sources and research on the role of carrier gases and humidity may allow their clinical application for wound management in the future.",
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Matthes, R, Hübner, NO, Bender, C, Koban, I, Horn, S, Bekeschus, S, Weltmann, KD, Kocher, T, Kramer, A & Assadian, O 2014, 'Efficacy of different carrier gases for barrier discharge plasma generation compared to chlorhexidine on the survival of pseudomonas aeruginosa embedded in biofilm in vitro', Skin Pharmacology and Physiology, vol. 27, no. 3, pp. 148-157. https://doi.org/10.1159/000353861

Efficacy of different carrier gases for barrier discharge plasma generation compared to chlorhexidine on the survival of pseudomonas aeruginosa embedded in biofilm in vitro. / Matthes, Rutger; Hübner, Nils Olaf; Bender, C.; Koban, I.; Horn, S.; Bekeschus, S.; Weltmann, K. D.; Kocher, T.; Kramer, Axel; Assadian, O.

In: Skin Pharmacology and Physiology, Vol. 27, No. 3, 02.2014, p. 148-157.

Research output: Contribution to journalArticle

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T1 - Efficacy of different carrier gases for barrier discharge plasma generation compared to chlorhexidine on the survival of pseudomonas aeruginosa embedded in biofilm in vitro

AU - Matthes, Rutger

AU - Hübner, Nils Olaf

AU - Bender, C.

AU - Koban, I.

AU - Horn, S.

AU - Bekeschus, S.

AU - Weltmann, K. D.

AU - Kocher, T.

AU - Kramer, Axel

AU - Assadian, O.

PY - 2014/2

Y1 - 2014/2

N2 - Because of its antimicrobial properties, nonthermal plasma could serve as an alternative to chemical antisepsis in wound treatment. Therefore, this study investigated the inactivation of biofilm-embedded Pseudomonas aeruginosa SG81 by a surface barrier-discharged (SBD) plasma for 30, 60, 150 and 300 s. In order to optimize the efficacy of the plasma, different carrier gases (argon, argon admixed with 1% oxygen, and argon with increased humidity up to approx. 80%) were tested and compared against 0.1% chlorhexidine digluconate (CHG) exposure for 600 s. The antimicrobial efficacy was determined by calculating the difference between the numbers of colony-forming units (CFU) of treated and untreated biofilms. Living bacteria were distinguished from dead by fluorescent staining and confocal laser scanning microscopy. Both SBD plasmas and CHG showed significant antimicrobial effects compared to the untreated control. However, plasma treatment led to a higher antimicrobial reduction (argon plasma 4.9 log10 CFU/cm2, argon with admixed oxygen 3 log10 CFU/cm2, and with increased gas humidity 2.7 log10 CFU/cm2 after 300 s) compared to CHG. In conclusion, SBD plasma is suitable as an alternative to CHG for inactivation of Pseudomonas aeruginosa embedded in biofilm. Further development of SBD plasma sources and research on the role of carrier gases and humidity may allow their clinical application for wound management in the future.

AB - Because of its antimicrobial properties, nonthermal plasma could serve as an alternative to chemical antisepsis in wound treatment. Therefore, this study investigated the inactivation of biofilm-embedded Pseudomonas aeruginosa SG81 by a surface barrier-discharged (SBD) plasma for 30, 60, 150 and 300 s. In order to optimize the efficacy of the plasma, different carrier gases (argon, argon admixed with 1% oxygen, and argon with increased humidity up to approx. 80%) were tested and compared against 0.1% chlorhexidine digluconate (CHG) exposure for 600 s. The antimicrobial efficacy was determined by calculating the difference between the numbers of colony-forming units (CFU) of treated and untreated biofilms. Living bacteria were distinguished from dead by fluorescent staining and confocal laser scanning microscopy. Both SBD plasmas and CHG showed significant antimicrobial effects compared to the untreated control. However, plasma treatment led to a higher antimicrobial reduction (argon plasma 4.9 log10 CFU/cm2, argon with admixed oxygen 3 log10 CFU/cm2, and with increased gas humidity 2.7 log10 CFU/cm2 after 300 s) compared to CHG. In conclusion, SBD plasma is suitable as an alternative to CHG for inactivation of Pseudomonas aeruginosa embedded in biofilm. Further development of SBD plasma sources and research on the role of carrier gases and humidity may allow their clinical application for wound management in the future.

KW - Antimicrobial

KW - Atmospheric pressure plasma

KW - Biofilm

KW - Dielectric barrier discharge

KW - Pseudomonas aeruginosa

KW - Surface barrier discharge plasma source

KW - Wound management

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