Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide

G. A. Morris, S.N.G. Butler, T. J. Foster, K. Jumel, S. E. Harding

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Relatively little has been published on the ultracentrifuge behaviour of macromolecular solutions at elevated temperature (> 40°C). In this study we look at the sedimentation velocity behaviour of one particular food grade polyuronide - pectin - from 20°C to 60°C in a specially adapted Model E ultracentrifuge. Reduced specific viscosity measurements were also determined over the same temperature range. A small decrease in the reduced viscosity, together with a similar increase in s20,w, suggests that the pectin chain is more flexible at elevated temperatures, but that the overall molecular integrity remains intact.

Original languageEnglish
Title of host publicationAnalytical Ultracentrifugation V
Subtitle of host publicationProceedings of the 11th Symposium on Analytical Ultracentrifugation, (University of Potsdam, Germany, 25-26 March 1999)
EditorsHelmut Cölfen
PublisherSpringer Verlag
Pages205-208
Number of pages4
ISBN (Electronic)9783540487036
ISBN (Print)9783540661757
DOIs
Publication statusPublished - 28 Sep 1999
Externally publishedYes
Event11th Symposium on Analytical Ultracentrifugation - University of Potsdam, Germany
Duration: 25 Mar 199926 Mar 1999

Publication series

NameProgress in Colloid and Polymer Science
PublisherSpringer
Volume113
ISSN (Print)0340-255X

Conference

Conference11th Symposium on Analytical Ultracentrifugation
CountryGermany
Period25/03/9926/03/99

Fingerprint

viscosity
Viscosity measurement
Sedimentation
food
integrity
Temperature
temperature
grade
Viscosity
Ultracentrifugation
traumacel
pectin

Cite this

Morris, G. A., Butler, S. N. G., Foster, T. J., Jumel, K., & Harding, S. E. (1999). Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. In H. Cölfen (Ed.), Analytical Ultracentrifugation V: Proceedings of the 11th Symposium on Analytical Ultracentrifugation, (University of Potsdam, Germany, 25-26 March 1999) (pp. 205-208). (Progress in Colloid and Polymer Science; Vol. 113). Springer Verlag. Progress in Colloid and Polymer Science https://doi.org/10.1007/3-540-48703-4
Morris, G. A. ; Butler, S.N.G. ; Foster, T. J. ; Jumel, K. ; Harding, S. E. / Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. Analytical Ultracentrifugation V: Proceedings of the 11th Symposium on Analytical Ultracentrifugation, (University of Potsdam, Germany, 25-26 March 1999). editor / Helmut Cölfen. Springer Verlag, 1999. pp. 205-208 (Progress in Colloid and Polymer Science). (Progress in Colloid and Polymer Science).
@inproceedings{bc983c527c944b78abd0a8849cf7a199,
title = "Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide",
abstract = "Relatively little has been published on the ultracentrifuge behaviour of macromolecular solutions at elevated temperature (> 40°C). In this study we look at the sedimentation velocity behaviour of one particular food grade polyuronide - pectin - from 20°C to 60°C in a specially adapted Model E ultracentrifuge. Reduced specific viscosity measurements were also determined over the same temperature range. A small decrease in the reduced viscosity, together with a similar increase in s20,w, suggests that the pectin chain is more flexible at elevated temperatures, but that the overall molecular integrity remains intact.",
keywords = "Beckman Model E adaptation, Size exclusion chromatography-multiangle laser light scattering, Viscometry",
author = "Morris, {G. A.} and S.N.G. Butler and Foster, {T. J.} and K. Jumel and Harding, {S. E.}",
year = "1999",
month = "9",
day = "28",
doi = "10.1007/3-540-48703-4",
language = "English",
isbn = "9783540661757",
series = "Progress in Colloid and Polymer Science",
publisher = "Springer Verlag",
pages = "205--208",
editor = "Helmut C{\"o}lfen",
booktitle = "Analytical Ultracentrifugation V",

}

Morris, GA, Butler, SNG, Foster, TJ, Jumel, K & Harding, SE 1999, Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. in H Cölfen (ed.), Analytical Ultracentrifugation V: Proceedings of the 11th Symposium on Analytical Ultracentrifugation, (University of Potsdam, Germany, 25-26 March 1999). Progress in Colloid and Polymer Science, vol. 113, Springer Verlag, Progress in Colloid and Polymer Science, pp. 205-208, 11th Symposium on Analytical Ultracentrifugation, Germany, 25/03/99. https://doi.org/10.1007/3-540-48703-4

Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. / Morris, G. A.; Butler, S.N.G.; Foster, T. J.; Jumel, K.; Harding, S. E.

Analytical Ultracentrifugation V: Proceedings of the 11th Symposium on Analytical Ultracentrifugation, (University of Potsdam, Germany, 25-26 March 1999). ed. / Helmut Cölfen. Springer Verlag, 1999. p. 205-208 (Progress in Colloid and Polymer Science; Vol. 113), (Progress in Colloid and Polymer Science).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide

AU - Morris, G. A.

AU - Butler, S.N.G.

AU - Foster, T. J.

AU - Jumel, K.

AU - Harding, S. E.

PY - 1999/9/28

Y1 - 1999/9/28

N2 - Relatively little has been published on the ultracentrifuge behaviour of macromolecular solutions at elevated temperature (> 40°C). In this study we look at the sedimentation velocity behaviour of one particular food grade polyuronide - pectin - from 20°C to 60°C in a specially adapted Model E ultracentrifuge. Reduced specific viscosity measurements were also determined over the same temperature range. A small decrease in the reduced viscosity, together with a similar increase in s20,w, suggests that the pectin chain is more flexible at elevated temperatures, but that the overall molecular integrity remains intact.

AB - Relatively little has been published on the ultracentrifuge behaviour of macromolecular solutions at elevated temperature (> 40°C). In this study we look at the sedimentation velocity behaviour of one particular food grade polyuronide - pectin - from 20°C to 60°C in a specially adapted Model E ultracentrifuge. Reduced specific viscosity measurements were also determined over the same temperature range. A small decrease in the reduced viscosity, together with a similar increase in s20,w, suggests that the pectin chain is more flexible at elevated temperatures, but that the overall molecular integrity remains intact.

KW - Beckman Model E adaptation

KW - Size exclusion chromatography-multiangle laser light scattering

KW - Viscometry

UR - http://www.scopus.com/inward/record.url?scp=0008437045&partnerID=8YFLogxK

UR - https://link.springer.com/bookseries/2882

U2 - 10.1007/3-540-48703-4

DO - 10.1007/3-540-48703-4

M3 - Conference contribution

SN - 9783540661757

T3 - Progress in Colloid and Polymer Science

SP - 205

EP - 208

BT - Analytical Ultracentrifugation V

A2 - Cölfen, Helmut

PB - Springer Verlag

ER -

Morris GA, Butler SNG, Foster TJ, Jumel K, Harding SE. Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide. In Cölfen H, editor, Analytical Ultracentrifugation V: Proceedings of the 11th Symposium on Analytical Ultracentrifugation, (University of Potsdam, Germany, 25-26 March 1999). Springer Verlag. 1999. p. 205-208. (Progress in Colloid and Polymer Science). (Progress in Colloid and Polymer Science). https://doi.org/10.1007/3-540-48703-4