Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer

Luke H. Stockwin; Torkjel Matzow; Nikolaos T. Georgopoulos; Lindsay J. Stanbridge; Samantha V. Jones; Iain G. Martin; Maria E. Blair-Zajdel; G. Eric Blair

doi:10.1016/S0022-1759(01)00510-5

Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer

Luke H. Stockwin, Torkjel Matzow, Nikolaos T. Georgopoulos, Lindsay J. Stanbridge, Samantha V. Jones, Iain G. Martin, Maria E. Blair-Zajdel, G. Eric Blair

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.

Original language	English
Pages (from-to)	205-215
Number of pages	11
Journal	Journal of Immunological Methods
Volume	259
Issue number	1-2
Early online date	18 Oct 2001
DOIs	https://doi.org/10.1016/S0022-1759(01)00510-5
Publication status	Published - 1 Jan 2002
Externally published	Yes

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Stockwin, L. H., Matzow, T., Georgopoulos, N. T., Stanbridge, L. J., Jones, S. V., Martin, I. G., Blair-Zajdel, M. E., & Blair, G. E. (2002). Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer. Journal of Immunological Methods, 259(1-2), 205-215. https://doi.org/10.1016/S0022-1759(01)00510-5

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title = "Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer",

abstract = "Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.",

keywords = "Adenovirus, CAR, Dendritic cell, Viral receptor, Viral uptake",

author = "Stockwin, {Luke H.} and Torkjel Matzow and Georgopoulos, {Nikolaos T.} and Stanbridge, {Lindsay J.} and Jones, {Samantha V.} and Martin, {Iain G.} and Blair-Zajdel, {Maria E.} and Blair, {G. Eric}",

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Stockwin, LH, Matzow, T, Georgopoulos, NT, Stanbridge, LJ, Jones, SV, Martin, IG, Blair-Zajdel, ME & Blair, GE 2002, 'Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer', Journal of Immunological Methods, vol. 259, no. 1-2, pp. 205-215. https://doi.org/10.1016/S0022-1759(01)00510-5

Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer. / Stockwin, Luke H.; Matzow, Torkjel; Georgopoulos, Nikolaos T. et al.

In: Journal of Immunological Methods, Vol. 259, No. 1-2, 01.01.2002, p. 205-215.

Research output: Contribution to journal › Article › peer-review

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T1 - Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer

AU - Stockwin, Luke H.

AU - Matzow, Torkjel

AU - Georgopoulos, Nikolaos T.

AU - Stanbridge, Lindsay J.

AU - Jones, Samantha V.

AU - Martin, Iain G.

AU - Blair-Zajdel, Maria E.

AU - Blair, G. Eric

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Y1 - 2002/1/1

N2 - Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.

AB - Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.

KW - Adenovirus

KW - CAR

KW - Dendritic cell

KW - Viral receptor

KW - Viral uptake

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Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer

Abstract

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