Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer

Luke H. Stockwin, Torkjel Matzow, Nikolaos T. Georgopoulos, Lindsay J. Stanbridge, Samantha V. Jones, Iain G. Martin, Maria E. Blair-Zajdel, G. Eric Blair

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.

Original languageEnglish
Pages (from-to)205-215
Number of pages11
JournalJournal of Immunological Methods
Volume259
Issue number1-2
Early online date18 Oct 2001
DOIs
Publication statusPublished - 1 Jan 2002
Externally publishedYes

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