Enhanced Chlorhexidine Skin Penetration with 1,8-cineole

Anna L. Casey, Tarja J. Karpanen, Barbara Conway, Tony Worthington, Peter Nightingale, R. Waters, Tom S J Elliott

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background:
Chlorhexidine (CHG) penetrates poorly into skin. The purpose of this study was to compare the depth of CHG skin permeation from solutions containing either 2% (w/v) CHG and 70% (v/v) isopropyl alcohol (IPA) or 2% (w/v) CHG, 70% (v/v) IPA and 2% (v/v) 1,8-cineole.

Methods:
An ex-vivo study using Franz diffusion cells was carried out. Full thickness human skin was mounted onto the cells and a CHG solution, with or without 2% (v/v) 1,8-cineole was applied to the skin surface. After twenty-four hours the skin was sectioned horizontally in 100 μm slices to a depth of 2000 μm and the concentration of CHG in each section quantified using high performance liquid chromatography (HPLC). The data were analysed with repeated measures analysis of variance.

Results:
The concentration of CHG in the skin on average was significantly higher (33.3% [95%, CI 1.5% - 74.9%]) when a CHG solution which contained 1,8-cineole was applied to the skin compared to a CHG solution which did not contain this terpene (P = 0.042).

Conclusions:
Enhanced delivery of CHG can be achieved in the presence of 1,8-cineole, which is the major component of eucalyptus oil. This may reduce the numbers of microorganisms located in the deeper layers of the skin which potentially could decrease the risk of surgical site infection.
LanguageEnglish
Number of pages5
JournalBMC Infectious Diseases
Volume17
Issue number350
DOIs
Publication statusPublished - 17 May 2017

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Chlorhexidine
Skin
2-Propanol
Surgical Wound Infection
Eucalyptus
eucalyptol
Terpenes
Analysis of Variance
Oils
High Pressure Liquid Chromatography

Cite this

Casey, A. L., Karpanen, T. J., Conway, B., Worthington, T., Nightingale, P., Waters, R., & Elliott, T. S. J. (2017). Enhanced Chlorhexidine Skin Penetration with 1,8-cineole. BMC Infectious Diseases, 17(350). https://doi.org/10.1186/s12879-017-2451-4
Casey, Anna L. ; Karpanen, Tarja J. ; Conway, Barbara ; Worthington, Tony ; Nightingale, Peter ; Waters, R. ; Elliott, Tom S J. / Enhanced Chlorhexidine Skin Penetration with 1,8-cineole. In: BMC Infectious Diseases. 2017 ; Vol. 17, No. 350.
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title = "Enhanced Chlorhexidine Skin Penetration with 1,8-cineole",
abstract = "Background:Chlorhexidine (CHG) penetrates poorly into skin. The purpose of this study was to compare the depth of CHG skin permeation from solutions containing either 2{\%} (w/v) CHG and 70{\%} (v/v) isopropyl alcohol (IPA) or 2{\%} (w/v) CHG, 70{\%} (v/v) IPA and 2{\%} (v/v) 1,8-cineole.Methods:An ex-vivo study using Franz diffusion cells was carried out. Full thickness human skin was mounted onto the cells and a CHG solution, with or without 2{\%} (v/v) 1,8-cineole was applied to the skin surface. After twenty-four hours the skin was sectioned horizontally in 100 μm slices to a depth of 2000 μm and the concentration of CHG in each section quantified using high performance liquid chromatography (HPLC). The data were analysed with repeated measures analysis of variance.Results:The concentration of CHG in the skin on average was significantly higher (33.3{\%} [95{\%}, CI 1.5{\%} - 74.9{\%}]) when a CHG solution which contained 1,8-cineole was applied to the skin compared to a CHG solution which did not contain this terpene (P = 0.042).Conclusions:Enhanced delivery of CHG can be achieved in the presence of 1,8-cineole, which is the major component of eucalyptus oil. This may reduce the numbers of microorganisms located in the deeper layers of the skin which potentially could decrease the risk of surgical site infection.",
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Casey, AL, Karpanen, TJ, Conway, B, Worthington, T, Nightingale, P, Waters, R & Elliott, TSJ 2017, 'Enhanced Chlorhexidine Skin Penetration with 1,8-cineole', BMC Infectious Diseases, vol. 17, no. 350. https://doi.org/10.1186/s12879-017-2451-4

Enhanced Chlorhexidine Skin Penetration with 1,8-cineole. / Casey, Anna L.; Karpanen, Tarja J.; Conway, Barbara; Worthington, Tony; Nightingale, Peter; Waters, R.; Elliott, Tom S J.

In: BMC Infectious Diseases, Vol. 17, No. 350, 17.05.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhanced Chlorhexidine Skin Penetration with 1,8-cineole

AU - Casey, Anna L.

AU - Karpanen, Tarja J.

AU - Conway, Barbara

AU - Worthington, Tony

AU - Nightingale, Peter

AU - Waters, R.

AU - Elliott, Tom S J

PY - 2017/5/17

Y1 - 2017/5/17

N2 - Background:Chlorhexidine (CHG) penetrates poorly into skin. The purpose of this study was to compare the depth of CHG skin permeation from solutions containing either 2% (w/v) CHG and 70% (v/v) isopropyl alcohol (IPA) or 2% (w/v) CHG, 70% (v/v) IPA and 2% (v/v) 1,8-cineole.Methods:An ex-vivo study using Franz diffusion cells was carried out. Full thickness human skin was mounted onto the cells and a CHG solution, with or without 2% (v/v) 1,8-cineole was applied to the skin surface. After twenty-four hours the skin was sectioned horizontally in 100 μm slices to a depth of 2000 μm and the concentration of CHG in each section quantified using high performance liquid chromatography (HPLC). The data were analysed with repeated measures analysis of variance.Results:The concentration of CHG in the skin on average was significantly higher (33.3% [95%, CI 1.5% - 74.9%]) when a CHG solution which contained 1,8-cineole was applied to the skin compared to a CHG solution which did not contain this terpene (P = 0.042).Conclusions:Enhanced delivery of CHG can be achieved in the presence of 1,8-cineole, which is the major component of eucalyptus oil. This may reduce the numbers of microorganisms located in the deeper layers of the skin which potentially could decrease the risk of surgical site infection.

AB - Background:Chlorhexidine (CHG) penetrates poorly into skin. The purpose of this study was to compare the depth of CHG skin permeation from solutions containing either 2% (w/v) CHG and 70% (v/v) isopropyl alcohol (IPA) or 2% (w/v) CHG, 70% (v/v) IPA and 2% (v/v) 1,8-cineole.Methods:An ex-vivo study using Franz diffusion cells was carried out. Full thickness human skin was mounted onto the cells and a CHG solution, with or without 2% (v/v) 1,8-cineole was applied to the skin surface. After twenty-four hours the skin was sectioned horizontally in 100 μm slices to a depth of 2000 μm and the concentration of CHG in each section quantified using high performance liquid chromatography (HPLC). The data were analysed with repeated measures analysis of variance.Results:The concentration of CHG in the skin on average was significantly higher (33.3% [95%, CI 1.5% - 74.9%]) when a CHG solution which contained 1,8-cineole was applied to the skin compared to a CHG solution which did not contain this terpene (P = 0.042).Conclusions:Enhanced delivery of CHG can be achieved in the presence of 1,8-cineole, which is the major component of eucalyptus oil. This may reduce the numbers of microorganisms located in the deeper layers of the skin which potentially could decrease the risk of surgical site infection.

KW - Skin antisepsis

KW - Antiseptic penetration

KW - Terpene

UR - https://bmcinfectdis.biomedcentral.com/

U2 - 10.1186/s12879-017-2451-4

DO - 10.1186/s12879-017-2451-4

M3 - Article

VL - 17

JO - BMC Infectious Diseases

T2 - BMC Infectious Diseases

JF - BMC Infectious Diseases

SN - 1471-2334

IS - 350

ER -

Casey AL, Karpanen TJ, Conway B, Worthington T, Nightingale P, Waters R et al. Enhanced Chlorhexidine Skin Penetration with 1,8-cineole. BMC Infectious Diseases. 2017 May 17;17(350). https://doi.org/10.1186/s12879-017-2451-4