Enhanced chlorhexidine skin penetration with eucalyptus oil

Tarja J. Karpanen, Barbara R. Conway, Tony Worthington, Anthony C. Hilton, Tom S J Elliott, Peter A. Lambert

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Chlorhexidine digluconate (CHG) is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG) when used as a skin antiseptic.Method: Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2% (w/v) CHG in various concentrations of eucalyptus oil (EO) and 70% (v/v) isopropyl alcohol (IPA). The concentration of CHG (μg/mg of skin) was determined to a skin depth of 1500 μm by high performance liquid chromatography (HPLC).Results: The 2% (w/v) CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v) EO in combination with 2% (w/v) CHG in 70% (v/v) IPA significantly increased the amount of CHG which penetrated into the skin within 2 min.Conclusion: The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide contact with additional microorganisms present in the skin, thereby enhancing antisepsis.

Original languageEnglish
Article number278
JournalBMC Infectious Diseases
Volume10
DOIs
Publication statusPublished - 22 Sep 2010

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Eucalyptus
Chlorhexidine
Oils
Skin
Local Anti-Infective Agents
2-Propanol
Antisepsis
chlorhexidine gluconate
Disinfectants
Dermis
Epidermis

Cite this

Karpanen, T. J., Conway, B. R., Worthington, T., Hilton, A. C., Elliott, T. S. J., & Lambert, P. A. (2010). Enhanced chlorhexidine skin penetration with eucalyptus oil. BMC Infectious Diseases, 10, [278]. https://doi.org/10.1186/1471-2334-10-278
Karpanen, Tarja J. ; Conway, Barbara R. ; Worthington, Tony ; Hilton, Anthony C. ; Elliott, Tom S J ; Lambert, Peter A. / Enhanced chlorhexidine skin penetration with eucalyptus oil. In: BMC Infectious Diseases. 2010 ; Vol. 10.
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title = "Enhanced chlorhexidine skin penetration with eucalyptus oil",
abstract = "Background: Chlorhexidine digluconate (CHG) is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG) when used as a skin antiseptic.Method: Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2{\%} (w/v) CHG in various concentrations of eucalyptus oil (EO) and 70{\%} (v/v) isopropyl alcohol (IPA). The concentration of CHG (μg/mg of skin) was determined to a skin depth of 1500 μm by high performance liquid chromatography (HPLC).Results: The 2{\%} (w/v) CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v) EO in combination with 2{\%} (w/v) CHG in 70{\%} (v/v) IPA significantly increased the amount of CHG which penetrated into the skin within 2 min.Conclusion: The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide contact with additional microorganisms present in the skin, thereby enhancing antisepsis.",
keywords = "High Performance Liquid Chromatography (HPLC), stratum corneum, Chlorhexidine, skin penetration, intravascular catheter",
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Karpanen, TJ, Conway, BR, Worthington, T, Hilton, AC, Elliott, TSJ & Lambert, PA 2010, 'Enhanced chlorhexidine skin penetration with eucalyptus oil', BMC Infectious Diseases, vol. 10, 278. https://doi.org/10.1186/1471-2334-10-278

Enhanced chlorhexidine skin penetration with eucalyptus oil. / Karpanen, Tarja J.; Conway, Barbara R.; Worthington, Tony; Hilton, Anthony C.; Elliott, Tom S J; Lambert, Peter A.

In: BMC Infectious Diseases, Vol. 10, 278, 22.09.2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhanced chlorhexidine skin penetration with eucalyptus oil

AU - Karpanen, Tarja J.

AU - Conway, Barbara R.

AU - Worthington, Tony

AU - Hilton, Anthony C.

AU - Elliott, Tom S J

AU - Lambert, Peter A.

PY - 2010/9/22

Y1 - 2010/9/22

N2 - Background: Chlorhexidine digluconate (CHG) is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG) when used as a skin antiseptic.Method: Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2% (w/v) CHG in various concentrations of eucalyptus oil (EO) and 70% (v/v) isopropyl alcohol (IPA). The concentration of CHG (μg/mg of skin) was determined to a skin depth of 1500 μm by high performance liquid chromatography (HPLC).Results: The 2% (w/v) CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v) EO in combination with 2% (w/v) CHG in 70% (v/v) IPA significantly increased the amount of CHG which penetrated into the skin within 2 min.Conclusion: The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide contact with additional microorganisms present in the skin, thereby enhancing antisepsis.

AB - Background: Chlorhexidine digluconate (CHG) is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG) when used as a skin antiseptic.Method: Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2% (w/v) CHG in various concentrations of eucalyptus oil (EO) and 70% (v/v) isopropyl alcohol (IPA). The concentration of CHG (μg/mg of skin) was determined to a skin depth of 1500 μm by high performance liquid chromatography (HPLC).Results: The 2% (w/v) CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v) EO in combination with 2% (w/v) CHG in 70% (v/v) IPA significantly increased the amount of CHG which penetrated into the skin within 2 min.Conclusion: The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide contact with additional microorganisms present in the skin, thereby enhancing antisepsis.

KW - High Performance Liquid Chromatography (HPLC)

KW - stratum corneum

KW - Chlorhexidine

KW - skin penetration

KW - intravascular catheter

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U2 - 10.1186/1471-2334-10-278

DO - 10.1186/1471-2334-10-278

M3 - Article

VL - 10

JO - BMC Infectious Diseases

JF - BMC Infectious Diseases

SN - 1471-2334

M1 - 278

ER -

Karpanen TJ, Conway BR, Worthington T, Hilton AC, Elliott TSJ, Lambert PA. Enhanced chlorhexidine skin penetration with eucalyptus oil. BMC Infectious Diseases. 2010 Sep 22;10. 278. https://doi.org/10.1186/1471-2334-10-278