Enhanced fluorescence quenching rate of Coumarin 102 in stimulated emission depletion

Wenxuan Zhao, Wei Zhao, Nityanand Sharma, Chen Zhang, Xiaoqiang Feng, Haiyun Qin, Cuifang Kuang, Kaige Wang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The fluorescence quenching rate (KQ) of fluorescence dye in continuous-wave stimulated emission depletion (CW-STED) system is experimentally investigated by utilizing a commercial dye (Coumarin 102). With the plotting of a power-dependence curve, it is found that the quenching rate is increasing rather than staying steady with the raising of depletion beam power in CW STED. Moreover, increasing fluorescence dye concentration and excitation power could also result in the increasing of KQ, which gives rise to the negative influence on spatial resolution of CW STED microscope. The results are helpful in understanding the influence of quenching on CW STED, improving the performance of CW STED microscope and developing new STED-compatible fluorescent dye.

Original languageEnglish
Article number116982
Number of pages7
JournalJournal of Luminescence
Early online date24 Dec 2019
Publication statusPublished - 1 Apr 2020
Externally publishedYes


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