Evaluation of different lignocellulosic biomass pretreatments by phenotypic microarray-based metabolic analysis of fermenting yeast

Stuart Wilkinson, Darren Greetham, Gregory A. Tucker

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Advanced generation biofuel production from lignocellulosic material (LCM) was investigated. A range of different thermo-chemical pre-treatments were evaluated with different LCM. The pre-treatments included; alkaline (5% NaOH at 50°C), acid (1% H2SO4 at 121°C) and autohydrolytical methods (200°C aqueous based hydrothermal) and were evaluated using samples of miscanthus, wheat-straw and willow. The liberation of sugars, presence of inhibitory compounds, and the degree of enhancement of enzymatic saccharification was accessed. The suitability of the pre-treatment generated hydrolysates (as bioethanol feedstocks for Saccharomyces cerevisiae) was also accessed using a phenotypic microarray that measured yeast metabolic output. The use of the alkaline pre-treatment liberated more glucose and arabinose into both the pre-treatment generated hydrolysate and also the hydrolysate produced after enzymatic hydrolysis (when compared with other pre-treatments). However, hydrolysates derived from use of alkaline pre-treatments were shown to be unsuitable as a fermentation medium due to issues with colloidal stability (high viscosity). Use of acid or autohydrolytical pre-treatments liberated high concentrations of monosaccharides regardless of the LCM used and the hydrolysates had good fermentation performance with measurable yeast metabolic output. Acid pre-treated wheat straw hydrolysates were then used as a model system for larger scale fermentations to confirm both the results of the phenotypic microarray and its validity as an effective high-throughput screening tool.

Original languageEnglish
Pages (from-to)357-365
Number of pages9
JournalBiofuel Research Journal
Volume3
Issue number1
Early online date1 Mar 2016
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

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Microarrays
Yeast
Biomass
Fermentation
Yeasts
Straw
Triticum
Acids
Salix
Saccharification
Bioethanol
Arabinose
Enzymatic hydrolysis
Biofuels
Monosaccharides
Viscosity
Sugars
Feedstocks
Glucose
Saccharomyces cerevisiae

Cite this

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abstract = "Advanced generation biofuel production from lignocellulosic material (LCM) was investigated. A range of different thermo-chemical pre-treatments were evaluated with different LCM. The pre-treatments included; alkaline (5{\%} NaOH at 50°C), acid (1{\%} H2SO4 at 121°C) and autohydrolytical methods (200°C aqueous based hydrothermal) and were evaluated using samples of miscanthus, wheat-straw and willow. The liberation of sugars, presence of inhibitory compounds, and the degree of enhancement of enzymatic saccharification was accessed. The suitability of the pre-treatment generated hydrolysates (as bioethanol feedstocks for Saccharomyces cerevisiae) was also accessed using a phenotypic microarray that measured yeast metabolic output. The use of the alkaline pre-treatment liberated more glucose and arabinose into both the pre-treatment generated hydrolysate and also the hydrolysate produced after enzymatic hydrolysis (when compared with other pre-treatments). However, hydrolysates derived from use of alkaline pre-treatments were shown to be unsuitable as a fermentation medium due to issues with colloidal stability (high viscosity). Use of acid or autohydrolytical pre-treatments liberated high concentrations of monosaccharides regardless of the LCM used and the hydrolysates had good fermentation performance with measurable yeast metabolic output. Acid pre-treated wheat straw hydrolysates were then used as a model system for larger scale fermentations to confirm both the results of the phenotypic microarray and its validity as an effective high-throughput screening tool.",
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Evaluation of different lignocellulosic biomass pretreatments by phenotypic microarray-based metabolic analysis of fermenting yeast. / Wilkinson, Stuart; Greetham, Darren; Tucker, Gregory A.

In: Biofuel Research Journal, Vol. 3, No. 1, 01.12.2016, p. 357-365.

Research output: Contribution to journalArticle

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AU - Greetham, Darren

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