Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface

Helena C. Murphy, Mika Ala-Korpela, John J. White, Alia Raoof, Jimmy D. Bell, Maria L. Barnard, Shamus P. Burns, Richard A. Iles

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study demonstrates that the use of high field 1H NMR spectroscopy permits individual detection of phosphatidylcholine and sphingomyelin molecules at the surface of native low density lipoprotein (LDL) particles. Distinct behaviour was observed for the choline head group -N(CH3)3 resonances of these different phospholipids revealing preferential immobilisation for phosphatidylcholine. This suggests the existence of reversible and irreversible phosphatidylcholine-apolipoprotein B interactions and is consistent with microdomain formation at the surface monolayer of LDL. The novel resonance assignment and results show that 1H NMR can provide efficient and practical means for future studies on the structure and dynamics at the LDL surface.
LanguageEnglish
Pages733-737
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume234
Issue number3
DOIs
Publication statusPublished - 29 May 1997
Externally publishedYes

Fingerprint

Sphingomyelins
Phosphatidylcholines
LDL Lipoproteins
Apolipoproteins B
Choline
Immobilization
Nuclear magnetic resonance spectroscopy
Monolayers
Phospholipids
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Molecules
Proton Magnetic Resonance Spectroscopy

Cite this

Murphy, Helena C. ; Ala-Korpela, Mika ; White, John J. ; Raoof, Alia ; Bell, Jimmy D. ; Barnard, Maria L. ; Burns, Shamus P. ; Iles, Richard A. / Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 234, No. 3. pp. 733-737.
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Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface. / Murphy, Helena C.; Ala-Korpela, Mika; White, John J.; Raoof, Alia; Bell, Jimmy D.; Barnard, Maria L.; Burns, Shamus P.; Iles, Richard A.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 3, 29.05.1997, p. 733-737.

Research output: Contribution to journalArticle

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