Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface

Helena C. Murphy; Mika Ala-Korpela; John J. White; Alia Raoof; Jimmy D. Bell; Maria L. Barnard; Shamus P. Burns; Richard A. Iles

doi:10.1006/bbrc.1997.6634

Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface

Helena C. Murphy, Mika Ala-Korpela, John J. White, Alia Raoof, Jimmy D. Bell, Maria L. Barnard, Shamus P. Burns, Richard A. Iles

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

This study demonstrates that the use of high field 1H NMR spectroscopy permits individual detection of phosphatidylcholine and sphingomyelin molecules at the surface of native low density lipoprotein (LDL) particles. Distinct behaviour was observed for the choline head group -N(CH3)3 resonances of these different phospholipids revealing preferential immobilisation for phosphatidylcholine. This suggests the existence of reversible and irreversible phosphatidylcholine-apolipoprotein B interactions and is consistent with microdomain formation at the surface monolayer of LDL. The novel resonance assignment and results show that 1H NMR can provide efficient and practical means for future studies on the structure and dynamics at the LDL surface.

Original language	English
Pages (from-to)	733-737
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	234
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1997.6634
Publication status	Published - 29 May 1997
Externally published	Yes

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title = "Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface",

abstract = "This study demonstrates that the use of high field 1H NMR spectroscopy permits individual detection of phosphatidylcholine and sphingomyelin molecules at the surface of native low density lipoprotein (LDL) particles. Distinct behaviour was observed for the choline head group -N(CH3)3 resonances of these different phospholipids revealing preferential immobilisation for phosphatidylcholine. This suggests the existence of reversible and irreversible phosphatidylcholine-apolipoprotein B interactions and is consistent with microdomain formation at the surface monolayer of LDL. The novel resonance assignment and results show that 1H NMR can provide efficient and practical means for future studies on the structure and dynamics at the LDL surface.",

author = "Murphy, {Helena C.} and Mika Ala-Korpela and White, {John J.} and Alia Raoof and Bell, {Jimmy D.} and Barnard, {Maria L.} and Burns, {Shamus P.} and Iles, {Richard A.}",

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Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface. / Murphy, Helena C.; Ala-Korpela, Mika; White, John J.; Raoof, Alia; Bell, Jimmy D.; Barnard, Maria L.; Burns, Shamus P.; Iles, Richard A.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 3, 29.05.1997, p. 733-737.

Research output: Contribution to journal › Article › peer-review

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AU - Murphy, Helena C.

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AU - Raoof, Alia

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AU - Barnard, Maria L.

AU - Burns, Shamus P.

AU - Iles, Richard A.

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