TY - JOUR
T1 - Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure
AU - Marshall, V M
AU - Laws, A P
AU - Gu, Y
AU - Levander, F
AU - Rådström, P
AU - De Vuyst, L
AU - Degeest, B
AU - Vaningelgem, F
AU - Dunn, H
AU - Elvin, M
PY - 2001/6
Y1 - 2001/6
N2 - AIMS: To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).METHODS AND RESULTS: The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.CONCLUSION: Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.SIGNIFICANCE AND IMPACT OF THE STUDY: This information should help in an understanding of genetic exchanges in lactic acid bacteria.
AB - AIMS: To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).METHODS AND RESULTS: The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.CONCLUSION: Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.SIGNIFICANCE AND IMPACT OF THE STUDY: This information should help in an understanding of genetic exchanges in lactic acid bacteria.
KW - Carbohydrate Sequence
KW - Galactose/metabolism
KW - Genotype
KW - Lactic Acid/metabolism
KW - Lactobacillus/genetics
KW - Molecular Conformation
KW - Polysaccharides, Bacterial/chemistry
KW - Streptococcus/genetics
KW - Transduction, Genetic
UR - https://www2.scopus.com/record/display.uri?eid=2-s2.0-0035018567&origin=resultslist&sort=plf-f&src=s&st1=Exopolysaccharide-producing+strains+of+thermophilic+lactic+acid+bacteria+cluster+into+groups+according+to+their+EPS+structure&st2=&sid=d4c79d946c284df8d893d6fcf60a52aa&sot=b&sdt=b&sl=140&s=TITLE-ABS-KEY%28Exopolysaccharide-producing+strains+of+thermophilic+lactic+acid+bacteria+cluster+into+groups+according+to+their+EPS+structure%29&relpos=0&citeCnt=49&searchTerm=
U2 - 10.1046/j.1472-765X.2001.00937.x
DO - 10.1046/j.1472-765X.2001.00937.x
M3 - Article
C2 - 11412358
VL - 32
SP - 433
EP - 437
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 6
ER -