Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure

V M Marshall, A P Laws, Y Gu, F Levander, P Rådström, L De Vuyst, B Degeest, F Vaningelgem, H Dunn, M Elvin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

AIMS: To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).

METHODS AND RESULTS: The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.

CONCLUSION: Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.

SIGNIFICANCE AND IMPACT OF THE STUDY: This information should help in an understanding of genetic exchanges in lactic acid bacteria.

Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalLetters in Applied Microbiology
Volume32
Issue number6
DOIs
Publication statusPublished - Jun 2001

Fingerprint

Galactose
Lactic Acid
DNA Restriction Enzymes
Bacteria
Lactobacillus delbrueckii
Cultured Milk Products
Streptococcus thermophilus
DNA
Milk
Magnetic Resonance Spectroscopy

Cite this

Marshall, V M ; Laws, A P ; Gu, Y ; Levander, F ; Rådström, P ; De Vuyst, L ; Degeest, B ; Vaningelgem, F ; Dunn, H ; Elvin, M. / Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure. In: Letters in Applied Microbiology. 2001 ; Vol. 32, No. 6. pp. 433-437.
@article{dec916237a234b7584d68073923cb8f4,
title = "Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure",
abstract = "AIMS: To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).METHODS AND RESULTS: The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.CONCLUSION: Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.SIGNIFICANCE AND IMPACT OF THE STUDY: This information should help in an understanding of genetic exchanges in lactic acid bacteria.",
keywords = "Carbohydrate Sequence, Galactose/metabolism, Genotype, Lactic Acid/metabolism, Lactobacillus/genetics, Molecular Conformation, Polysaccharides, Bacterial/chemistry, Streptococcus/genetics, Transduction, Genetic",
author = "Marshall, {V M} and Laws, {A P} and Y Gu and F Levander and P R{\aa}dstr{\"o}m and {De Vuyst}, L and B Degeest and F Vaningelgem and H Dunn and M Elvin",
year = "2001",
month = "6",
doi = "10.1046/j.1472-765X.2001.00937.x",
language = "English",
volume = "32",
pages = "433--437",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "6",

}

Marshall, VM, Laws, AP, Gu, Y, Levander, F, Rådström, P, De Vuyst, L, Degeest, B, Vaningelgem, F, Dunn, H & Elvin, M 2001, 'Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure', Letters in Applied Microbiology, vol. 32, no. 6, pp. 433-437. https://doi.org/10.1046/j.1472-765X.2001.00937.x

Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure. / Marshall, V M; Laws, A P; Gu, Y; Levander, F; Rådström, P; De Vuyst, L; Degeest, B; Vaningelgem, F; Dunn, H; Elvin, M.

In: Letters in Applied Microbiology, Vol. 32, No. 6, 06.2001, p. 433-437.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure

AU - Marshall, V M

AU - Laws, A P

AU - Gu, Y

AU - Levander, F

AU - Rådström, P

AU - De Vuyst, L

AU - Degeest, B

AU - Vaningelgem, F

AU - Dunn, H

AU - Elvin, M

PY - 2001/6

Y1 - 2001/6

N2 - AIMS: To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).METHODS AND RESULTS: The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.CONCLUSION: Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.SIGNIFICANCE AND IMPACT OF THE STUDY: This information should help in an understanding of genetic exchanges in lactic acid bacteria.

AB - AIMS: To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).METHODS AND RESULTS: The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.CONCLUSION: Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.SIGNIFICANCE AND IMPACT OF THE STUDY: This information should help in an understanding of genetic exchanges in lactic acid bacteria.

KW - Carbohydrate Sequence

KW - Galactose/metabolism

KW - Genotype

KW - Lactic Acid/metabolism

KW - Lactobacillus/genetics

KW - Molecular Conformation

KW - Polysaccharides, Bacterial/chemistry

KW - Streptococcus/genetics

KW - Transduction, Genetic

UR - https://www2.scopus.com/record/display.uri?eid=2-s2.0-0035018567&origin=resultslist&sort=plf-f&src=s&st1=Exopolysaccharide-producing+strains+of+thermophilic+lactic+acid+bacteria+cluster+into+groups+according+to+their+EPS+structure&st2=&sid=d4c79d946c284df8d893d6fcf60a52aa&sot=b&sdt=b&sl=140&s=TITLE-ABS-KEY%28Exopolysaccharide-producing+strains+of+thermophilic+lactic+acid+bacteria+cluster+into+groups+according+to+their+EPS+structure%29&relpos=0&citeCnt=49&searchTerm=

U2 - 10.1046/j.1472-765X.2001.00937.x

DO - 10.1046/j.1472-765X.2001.00937.x

M3 - Article

VL - 32

SP - 433

EP - 437

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 6

ER -