Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes

Anestis Vlysidis, Chenyu Du, Colin Webb, Constantinos Theodoropoulos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

An unstructured kinetic model for the growth of Actinobacillus succinogenes on glycerol as the only carbon source is proposed in this study. The model describes cell growth considering both substrate and product inhibition. and its parameters are estimated by fitting the model predictions to experimental data. Substrate consumption and product formation rates are described by the Luedeking-Piret model. The main product of the process is succinic acid while by-products like acetate, formate and ethanol have very low concentrations. The main environmental factors that affect the bioprocess were examined and optimum conditions in terms of yield, final succinic acid concentration and productivity were evaluated by a factorial experimental procedure. The examined parameters were the supply of CO2, the pH level and the redox balance. Experiments using different initial glycerol concentrations at the "optimum" environmental conditions were carried out and simulation studies were performed using the proposed model.

Original languageEnglish
Title of host publicationAIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings
Publication statusPublished - 1 Dec 2008
Externally publishedYes
EventAmerican Institute of Chemical Engineers Annual Meeting - Philadelphia, United States
Duration: 16 Nov 200821 Nov 2008
Conference number: 100
https://www.aiche.org/conferences/aiche-annual-meeting/2008 (Link to Conference Information)

Conference

ConferenceAmerican Institute of Chemical Engineers Annual Meeting
Abbreviated titleAIChE
CountryUnited States
CityPhiladelphia
Period16/11/0821/11/08
Internet address

Fingerprint

Bioconversion
Succinic Acid
Glycerol
Acids
formic acid
Cell growth
Substrates
Byproducts
Acetates
Ethanol
Carbon
Productivity
Kinetics
Experiments

Cite this

Vlysidis, A., Du, C., Webb, C., & Theodoropoulos, C. (2008). Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. In AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings
Vlysidis, Anestis ; Du, Chenyu ; Webb, Colin ; Theodoropoulos, Constantinos. / Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings. 2008.
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Vlysidis, A, Du, C, Webb, C & Theodoropoulos, C 2008, Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. in AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings. American Institute of Chemical Engineers Annual Meeting, Philadelphia, United States, 16/11/08.

Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. / Vlysidis, Anestis; Du, Chenyu; Webb, Colin; Theodoropoulos, Constantinos.

AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings. 2008.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - An unstructured kinetic model for the growth of Actinobacillus succinogenes on glycerol as the only carbon source is proposed in this study. The model describes cell growth considering both substrate and product inhibition. and its parameters are estimated by fitting the model predictions to experimental data. Substrate consumption and product formation rates are described by the Luedeking-Piret model. The main product of the process is succinic acid while by-products like acetate, formate and ethanol have very low concentrations. The main environmental factors that affect the bioprocess were examined and optimum conditions in terms of yield, final succinic acid concentration and productivity were evaluated by a factorial experimental procedure. The examined parameters were the supply of CO2, the pH level and the redox balance. Experiments using different initial glycerol concentrations at the "optimum" environmental conditions were carried out and simulation studies were performed using the proposed model.

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Vlysidis A, Du C, Webb C, Theodoropoulos C. Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. In AIChE100 - 2008 AIChE Annual Meeting, Conference Proceedings. 2008