Expression of aquaporin water channels in human urothelial carcinoma

Correlation of AQP3 expression with tumour grade and stage

Peter C. Rubenwolf, Wolfgang Otto, Stefan Denzinger, Ferdinand Hofstädter, Wolf Wieland, Nikolaos T. Georgopoulos

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: To study the expression, localization and potential clinical significance of aquaporin water channels both in well-established urothelial cancer (UC) cell lines and in human bladder carcinoma specimens of different stages and grades and to discuss the clinical relevance of the findings. Methods: AQP transcript and protein expression by RT4, RT112 and T24 UC cell lines was investigated using reverse transcriptase polymerase chain reaction and immunofluorescence labelling. Immunohistochemistry (IHC) was used to assess AQP protein expression in 94 UC specimens of various grades and stages. Results: AQP3 and 9 transcripts were expressed in low-grade RT4 and RT112, but not in high-grade T24 cells. By contrast, AQP4 mRNA was absent in RT4, but expressed by RT112 and T24. Transcripts for AQP7 and 11 were detected in all three UC cell lines. Immunofluorescence microscopy confirmed the expression of AQP3, 4 and 7 at the protein level. By IHC, AQP3 was shown to be intensely expressed by 86 %, 66 % and 33 % of specimens of stage pTa, pT1 and pT2 tumours, respectively (p < 0.001). Whereas 100 % of G1 tumours were positive, only 73 % and 55 % of G2 and G3 tumours were found to express AQP3 (p = 0.004). Conclusions: This is the first study to demonstrate that several AQPs are expressed in UC. Our results indicate that there is a correlation between AQP3 protein expression and tumour stage and grade, with AQP3 expression being reduced or lost in tumours of higher grade and stage. Taken together with the available evidence from other studies, we conclude that AQPs may play a role in the progression of UC and, in particular, that this could be of prognostic value.

Original languageEnglish
Pages (from-to)991-997
Number of pages7
JournalWorld Journal of Urology
Volume32
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Aquaporins
Carcinoma
Neoplasms
Cell Line
Aquaporin 3
Immunohistochemistry
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Fluorescence Microscopy
Fluorescent Antibody Technique
Urinary Bladder

Cite this

Rubenwolf, Peter C. ; Otto, Wolfgang ; Denzinger, Stefan ; Hofstädter, Ferdinand ; Wieland, Wolf ; Georgopoulos, Nikolaos T. / Expression of aquaporin water channels in human urothelial carcinoma : Correlation of AQP3 expression with tumour grade and stage. In: World Journal of Urology. 2014 ; Vol. 32, No. 4. pp. 991-997.
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abstract = "Purpose: To study the expression, localization and potential clinical significance of aquaporin water channels both in well-established urothelial cancer (UC) cell lines and in human bladder carcinoma specimens of different stages and grades and to discuss the clinical relevance of the findings. Methods: AQP transcript and protein expression by RT4, RT112 and T24 UC cell lines was investigated using reverse transcriptase polymerase chain reaction and immunofluorescence labelling. Immunohistochemistry (IHC) was used to assess AQP protein expression in 94 UC specimens of various grades and stages. Results: AQP3 and 9 transcripts were expressed in low-grade RT4 and RT112, but not in high-grade T24 cells. By contrast, AQP4 mRNA was absent in RT4, but expressed by RT112 and T24. Transcripts for AQP7 and 11 were detected in all three UC cell lines. Immunofluorescence microscopy confirmed the expression of AQP3, 4 and 7 at the protein level. By IHC, AQP3 was shown to be intensely expressed by 86 {\%}, 66 {\%} and 33 {\%} of specimens of stage pTa, pT1 and pT2 tumours, respectively (p < 0.001). Whereas 100 {\%} of G1 tumours were positive, only 73 {\%} and 55 {\%} of G2 and G3 tumours were found to express AQP3 (p = 0.004). Conclusions: This is the first study to demonstrate that several AQPs are expressed in UC. Our results indicate that there is a correlation between AQP3 protein expression and tumour stage and grade, with AQP3 expression being reduced or lost in tumours of higher grade and stage. Taken together with the available evidence from other studies, we conclude that AQPs may play a role in the progression of UC and, in particular, that this could be of prognostic value.",
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Expression of aquaporin water channels in human urothelial carcinoma : Correlation of AQP3 expression with tumour grade and stage. / Rubenwolf, Peter C.; Otto, Wolfgang; Denzinger, Stefan; Hofstädter, Ferdinand; Wieland, Wolf; Georgopoulos, Nikolaos T.

In: World Journal of Urology, Vol. 32, No. 4, 2014, p. 991-997.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of aquaporin water channels in human urothelial carcinoma

T2 - Correlation of AQP3 expression with tumour grade and stage

AU - Rubenwolf, Peter C.

AU - Otto, Wolfgang

AU - Denzinger, Stefan

AU - Hofstädter, Ferdinand

AU - Wieland, Wolf

AU - Georgopoulos, Nikolaos T.

PY - 2014

Y1 - 2014

N2 - Purpose: To study the expression, localization and potential clinical significance of aquaporin water channels both in well-established urothelial cancer (UC) cell lines and in human bladder carcinoma specimens of different stages and grades and to discuss the clinical relevance of the findings. Methods: AQP transcript and protein expression by RT4, RT112 and T24 UC cell lines was investigated using reverse transcriptase polymerase chain reaction and immunofluorescence labelling. Immunohistochemistry (IHC) was used to assess AQP protein expression in 94 UC specimens of various grades and stages. Results: AQP3 and 9 transcripts were expressed in low-grade RT4 and RT112, but not in high-grade T24 cells. By contrast, AQP4 mRNA was absent in RT4, but expressed by RT112 and T24. Transcripts for AQP7 and 11 were detected in all three UC cell lines. Immunofluorescence microscopy confirmed the expression of AQP3, 4 and 7 at the protein level. By IHC, AQP3 was shown to be intensely expressed by 86 %, 66 % and 33 % of specimens of stage pTa, pT1 and pT2 tumours, respectively (p < 0.001). Whereas 100 % of G1 tumours were positive, only 73 % and 55 % of G2 and G3 tumours were found to express AQP3 (p = 0.004). Conclusions: This is the first study to demonstrate that several AQPs are expressed in UC. Our results indicate that there is a correlation between AQP3 protein expression and tumour stage and grade, with AQP3 expression being reduced or lost in tumours of higher grade and stage. Taken together with the available evidence from other studies, we conclude that AQPs may play a role in the progression of UC and, in particular, that this could be of prognostic value.

AB - Purpose: To study the expression, localization and potential clinical significance of aquaporin water channels both in well-established urothelial cancer (UC) cell lines and in human bladder carcinoma specimens of different stages and grades and to discuss the clinical relevance of the findings. Methods: AQP transcript and protein expression by RT4, RT112 and T24 UC cell lines was investigated using reverse transcriptase polymerase chain reaction and immunofluorescence labelling. Immunohistochemistry (IHC) was used to assess AQP protein expression in 94 UC specimens of various grades and stages. Results: AQP3 and 9 transcripts were expressed in low-grade RT4 and RT112, but not in high-grade T24 cells. By contrast, AQP4 mRNA was absent in RT4, but expressed by RT112 and T24. Transcripts for AQP7 and 11 were detected in all three UC cell lines. Immunofluorescence microscopy confirmed the expression of AQP3, 4 and 7 at the protein level. By IHC, AQP3 was shown to be intensely expressed by 86 %, 66 % and 33 % of specimens of stage pTa, pT1 and pT2 tumours, respectively (p < 0.001). Whereas 100 % of G1 tumours were positive, only 73 % and 55 % of G2 and G3 tumours were found to express AQP3 (p = 0.004). Conclusions: This is the first study to demonstrate that several AQPs are expressed in UC. Our results indicate that there is a correlation between AQP3 protein expression and tumour stage and grade, with AQP3 expression being reduced or lost in tumours of higher grade and stage. Taken together with the available evidence from other studies, we conclude that AQPs may play a role in the progression of UC and, in particular, that this could be of prognostic value.

KW - Aquaporins

KW - Biomarker

KW - Bladder cancer

KW - Carcinogenesis

KW - Cell lines

KW - Urothelial carcinoma

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