Expression of mitochondrial cytochrome c oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation

Vinod Kumar; Andrew J. Hart; Ethiraju R. Keerthiraju; Paul R. Waldron; Gregory A. Tucker; Darren Greetham

doi:10.1371/journal.pone.0139129

Expression of mitochondrial cytochrome c oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation

Vinod Kumar, Andrew J. Hart, Ethiraju R. Keerthiraju, Paul R. Waldron, Gregory A. Tucker, Darren Greetham

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Introduction
Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pretreatment of lignocellulosic material releases acetic acid and previous work identified a cytochromeoxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells in the presence of acetic acid.

Results: A Δcox20 strain was sensitive to the presence of acetic acid compared with the background strain. Over expressing COX20 using a tetracycline-regulatable expression vector system in a Δcox20 strain, resulted in tolerance to the presence of acetic acid and tolerance could b eablated with addition of tetracycline. Assays also revealed that overexpression improvedtolerance to the presence of hydrogen peroxide-induced oxidative stress.

Conclusion This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.

Original language	English
Article number	e0139129
Number of pages	16
Journal	PLoS One
Volume	10
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0139129
Publication status	Published - 1 Oct 2015
Externally published	Yes

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10.1371/journal.pone.0139129Licence: CC BY

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title = "Expression of mitochondrial cytochrome c oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation",

abstract = "IntroductionSaccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pretreatment of lignocellulosic material releases acetic acid and previous work identified a cytochromeoxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells in the presence of acetic acid.Results: A Δcox20 strain was sensitive to the presence of acetic acid compared with the background strain. Over expressing COX20 using a tetracycline-regulatable expression vector system in a Δcox20 strain, resulted in tolerance to the presence of acetic acid and tolerance could b eablated with addition of tetracycline. Assays also revealed that overexpression improvedtolerance to the presence of hydrogen peroxide-induced oxidative stress. Conclusion This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.",

keywords = "Yeast Fermentation, Saccharomyces cerevisiae, fermentable sugars",

author = "Vinod Kumar and Hart, {Andrew J.} and Keerthiraju, {Ethiraju R.} and Waldron, {Paul R.} and Tucker, {Gregory A.} and Darren Greetham",

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T1 - Expression of mitochondrial cytochrome c oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation

AU - Kumar, Vinod

AU - Hart, Andrew J.

AU - Keerthiraju, Ethiraju R.

AU - Waldron, Paul R.

AU - Tucker, Gregory A.

AU - Greetham, Darren

PY - 2015/10/1

Y1 - 2015/10/1

N2 - IntroductionSaccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pretreatment of lignocellulosic material releases acetic acid and previous work identified a cytochromeoxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells in the presence of acetic acid.Results: A Δcox20 strain was sensitive to the presence of acetic acid compared with the background strain. Over expressing COX20 using a tetracycline-regulatable expression vector system in a Δcox20 strain, resulted in tolerance to the presence of acetic acid and tolerance could b eablated with addition of tetracycline. Assays also revealed that overexpression improvedtolerance to the presence of hydrogen peroxide-induced oxidative stress. Conclusion This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.

AB - IntroductionSaccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pretreatment of lignocellulosic material releases acetic acid and previous work identified a cytochromeoxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells in the presence of acetic acid.Results: A Δcox20 strain was sensitive to the presence of acetic acid compared with the background strain. Over expressing COX20 using a tetracycline-regulatable expression vector system in a Δcox20 strain, resulted in tolerance to the presence of acetic acid and tolerance could b eablated with addition of tetracycline. Assays also revealed that overexpression improvedtolerance to the presence of hydrogen peroxide-induced oxidative stress. Conclusion This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.

KW - Yeast Fermentation

KW - Saccharomyces cerevisiae

KW - fermentable sugars

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Expression of mitochondrial cytochrome c oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation

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