Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress

V. Kumar, A. J. Hart, T. T. Wimalasena, G. A. Tucker, D. Greetham

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars during beverage or bioethanol fermentations. These fermentations are characterised by high osmotic stress on a yeast cell, with selected brewing fermentations beginning at 20-25% fermentable sugars and bioethanol fermentations at 13% fermentable sugars. 

Results: RCK2 encodes for a MAPKAP (MAPK-activated protein kinase) enzyme and was identified on a locus by QTL analysis in yeast cells under osmotic stress, RCK2 expression was placed under a tetracycline regulatable vector and rescued glucose, sorbitol or glycerol induced osmotic stress in an rck2 null strain. A strain overexpressing RCK2 had significantly faster fermentation rates when compared with the empty vector control strain. 

Conclusions: Presence of RCK2 increased rates of glucose utilisation (~40 g glucose in first 8 h) during a 15% glucose fermentation and concurrent production of ethanol when compared with empty vector controls. Tolerance to osmotic stress using the tetracycline regulatable vectors could be turned off with the addition of tetracycline returning a rck2 null strain back to osmotic sensitivity.

Original languageEnglish
Article number85
Number of pages10
JournalMicrobial Cell Factories
Volume14
Issue number1
Early online date12 Jun 2015
DOIs
Publication statusPublished - 12 Jun 2015
Externally publishedYes

Fingerprint

Osmotic Pressure
Yeast
Fermentation
Protein Kinases
Yeasts
Cells
Proteins
Glucose
Tetracycline
Sugars
Bioethanol
Brewing
Beverages
Sorbitol
Glycerol
Saccharomyces cerevisiae
Ethanol
Enzymes

Cite this

@article{dbb9a1bff6964126a6b66da0f6251054,
title = "Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress",
abstract = "Background: Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars during beverage or bioethanol fermentations. These fermentations are characterised by high osmotic stress on a yeast cell, with selected brewing fermentations beginning at 20-25{\%} fermentable sugars and bioethanol fermentations at 13{\%} fermentable sugars. Results: RCK2 encodes for a MAPKAP (MAPK-activated protein kinase) enzyme and was identified on a locus by QTL analysis in yeast cells under osmotic stress, RCK2 expression was placed under a tetracycline regulatable vector and rescued glucose, sorbitol or glycerol induced osmotic stress in an rck2 null strain. A strain overexpressing RCK2 had significantly faster fermentation rates when compared with the empty vector control strain. Conclusions: Presence of RCK2 increased rates of glucose utilisation (~40 g glucose in first 8 h) during a 15{\%} glucose fermentation and concurrent production of ethanol when compared with empty vector controls. Tolerance to osmotic stress using the tetracycline regulatable vectors could be turned off with the addition of tetracycline returning a rck2 null strain back to osmotic sensitivity.",
keywords = "Fermentation, Trehalose, Ethanol Production, Osmotic Stress, Fermentable Sugar",
author = "V. Kumar and Hart, {A. J.} and Wimalasena, {T. T.} and Tucker, {G. A.} and D. Greetham",
year = "2015",
month = "6",
day = "12",
doi = "10.1186/s12934-015-0276-7",
language = "English",
volume = "14",
journal = "Microbial Cell Factories",
issn = "1475-2859",
publisher = "BioMed Central",
number = "1",

}

Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress. / Kumar, V.; Hart, A. J.; Wimalasena, T. T.; Tucker, G. A.; Greetham, D.

In: Microbial Cell Factories, Vol. 14, No. 1, 85, 12.06.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress

AU - Kumar, V.

AU - Hart, A. J.

AU - Wimalasena, T. T.

AU - Tucker, G. A.

AU - Greetham, D.

PY - 2015/6/12

Y1 - 2015/6/12

N2 - Background: Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars during beverage or bioethanol fermentations. These fermentations are characterised by high osmotic stress on a yeast cell, with selected brewing fermentations beginning at 20-25% fermentable sugars and bioethanol fermentations at 13% fermentable sugars. Results: RCK2 encodes for a MAPKAP (MAPK-activated protein kinase) enzyme and was identified on a locus by QTL analysis in yeast cells under osmotic stress, RCK2 expression was placed under a tetracycline regulatable vector and rescued glucose, sorbitol or glycerol induced osmotic stress in an rck2 null strain. A strain overexpressing RCK2 had significantly faster fermentation rates when compared with the empty vector control strain. Conclusions: Presence of RCK2 increased rates of glucose utilisation (~40 g glucose in first 8 h) during a 15% glucose fermentation and concurrent production of ethanol when compared with empty vector controls. Tolerance to osmotic stress using the tetracycline regulatable vectors could be turned off with the addition of tetracycline returning a rck2 null strain back to osmotic sensitivity.

AB - Background: Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars during beverage or bioethanol fermentations. These fermentations are characterised by high osmotic stress on a yeast cell, with selected brewing fermentations beginning at 20-25% fermentable sugars and bioethanol fermentations at 13% fermentable sugars. Results: RCK2 encodes for a MAPKAP (MAPK-activated protein kinase) enzyme and was identified on a locus by QTL analysis in yeast cells under osmotic stress, RCK2 expression was placed under a tetracycline regulatable vector and rescued glucose, sorbitol or glycerol induced osmotic stress in an rck2 null strain. A strain overexpressing RCK2 had significantly faster fermentation rates when compared with the empty vector control strain. Conclusions: Presence of RCK2 increased rates of glucose utilisation (~40 g glucose in first 8 h) during a 15% glucose fermentation and concurrent production of ethanol when compared with empty vector controls. Tolerance to osmotic stress using the tetracycline regulatable vectors could be turned off with the addition of tetracycline returning a rck2 null strain back to osmotic sensitivity.

KW - Fermentation

KW - Trehalose

KW - Ethanol Production

KW - Osmotic Stress

KW - Fermentable Sugar

UR - http://www.scopus.com/inward/record.url?scp=84934949970&partnerID=8YFLogxK

U2 - 10.1186/s12934-015-0276-7

DO - 10.1186/s12934-015-0276-7

M3 - Article

C2 - 26062605

AN - SCOPUS:84934949970

VL - 14

JO - Microbial Cell Factories

JF - Microbial Cell Factories

SN - 1475-2859

IS - 1

M1 - 85

ER -