Abstract
Original language | English |
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Pages (from-to) | 1326-1332 |
Number of pages | 7 |
Journal | Diabetes |
Volume | 52 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2003 |
Externally published | Yes |
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Fetal programming of perivenous glucose uptake reveals a regulatory mechanism governing hepatic glucose output during refeeding. / Murphy, Helena C.; Regan, Gemma; Bogdarina, Irina G.; Clark, Adrian J.L.; Iles, Richard A.; Cohen, Robert D.; Hitman, Graham A.; Berry, Colin L.; Coade, Zoe; Petry, Clive J.; Burns, Shamus P.
In: Diabetes, Vol. 52, No. 6, 06.2003, p. 1326-1332.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fetal programming of perivenous glucose uptake reveals a regulatory mechanism governing hepatic glucose output during refeeding
AU - Murphy, Helena C.
AU - Regan, Gemma
AU - Bogdarina, Irina G.
AU - Clark, Adrian J.L.
AU - Iles, Richard A.
AU - Cohen, Robert D.
AU - Hitman, Graham A.
AU - Berry, Colin L.
AU - Coade, Zoe
AU - Petry, Clive J.
AU - Burns, Shamus P.
PY - 2003/6
Y1 - 2003/6
N2 - Increased hepatic gluconeogenesis maintains glycemia during fasting and has been considered responsible for elevated hepatic glucose output in type 2 diabetes. Glucose derived periportally via gluconeogenesis is partially taken up perivenously in perfused liver but not in adult rats whose mothers were protein-restricted during gestation (MLP rats)-an environmental model of fetal programming of adult glucose intolerance exhibiting diminished perivenous glucokinase (GK) activity. We now show that perivenous glucose uptake rises with increasing glucose concentration (0-8 mmol/l) in control but not MLP liver, indicating that GK is fluxgenerating. The data demonstrate that acute control of hepatic glucose output is principally achieved by increasing perivenous glucose uptake, with rising glucose concentration during refeeding, rather than by downregulation of gluconeogenesis, which occurs in different hepatocytes. Consistent with these observations, glycogen synthesis in vivo commenced in the perivenous cells during refeeding, MLP livers accumulating less glycogen than controls. GK gene transcription was unchanged in MLP liver, the data supporting a recently proposed posttranscriptional model of GK regulation involving nuclear-cytoplasmic transport. The results are pertinent to impaired regulation of hepatic glucose output in type 2 diabetes, which could arise from diminished GK-mediated glucose uptake rather than increased gluconeogenesis.
AB - Increased hepatic gluconeogenesis maintains glycemia during fasting and has been considered responsible for elevated hepatic glucose output in type 2 diabetes. Glucose derived periportally via gluconeogenesis is partially taken up perivenously in perfused liver but not in adult rats whose mothers were protein-restricted during gestation (MLP rats)-an environmental model of fetal programming of adult glucose intolerance exhibiting diminished perivenous glucokinase (GK) activity. We now show that perivenous glucose uptake rises with increasing glucose concentration (0-8 mmol/l) in control but not MLP liver, indicating that GK is fluxgenerating. The data demonstrate that acute control of hepatic glucose output is principally achieved by increasing perivenous glucose uptake, with rising glucose concentration during refeeding, rather than by downregulation of gluconeogenesis, which occurs in different hepatocytes. Consistent with these observations, glycogen synthesis in vivo commenced in the perivenous cells during refeeding, MLP livers accumulating less glycogen than controls. GK gene transcription was unchanged in MLP liver, the data supporting a recently proposed posttranscriptional model of GK regulation involving nuclear-cytoplasmic transport. The results are pertinent to impaired regulation of hepatic glucose output in type 2 diabetes, which could arise from diminished GK-mediated glucose uptake rather than increased gluconeogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0037983795&partnerID=8YFLogxK
U2 - 10.2337/diabetes.52.6.1326
DO - 10.2337/diabetes.52.6.1326
M3 - Article
VL - 52
SP - 1326
EP - 1332
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 6
ER -