Abstract
| Language | English |
|---|---|
| Pages | 21-30 |
| Number of pages | 10 |
| Journal | Journal of Proteomics |
| Volume | 177 |
| Early online date | 31 Jan 2018 |
| DOIs | |
| Publication status | Published - 15 Apr 2018 |
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Forensic proteomics for the evaluation of the post-mortem decay in bones. / Procopio, Noemi; Williams, Anna; Chamberlain, Andrew T.; Buckley, Michael.
In: Journal of Proteomics, Vol. 177, 15.04.2018, p. 21-30.Research output: Contribution to journal › Article
TY - JOUR
T1 - Forensic proteomics for the evaluation of the post-mortem decay in bones
AU - Procopio, Noemi
AU - Williams, Anna
AU - Chamberlain, Andrew T.
AU - Buckley, Michael
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Current methods for the evaluation of post-mortem interval (PMI) of skeletal remains suffer from poor accuracy, due to the great number of variables that affect the diagenetic process and to the lack of specific guidelines to address this issue. During decomposition, proteins can undergo cumulative decay over the time, resulting in a decrease in the range and abundance of proteins present (i.e., the proteome) in different tissues as well as in an increase of post-translational modifications occurring on these proteins. In this study, we evaluate the applicability of bone proteomic analyses to simulated forensic contexts, looking for specific biomarkers that may help the estimation of PMI and evaluating a previously discovered marker for the estimation of biological age. We noticed a reduction of specific plasma and muscle proteins with increasing PMIs, as well as an increased deamidation of biglycan, a protein with a role in modulating bone growth and mineralization, which may be developed further for the estimation of PMI from a molecular perspective. We also corroborated our previous results regarding the use of fetuin-A as a potential biomarker for the estimation of age-at-death, demonstrating the applicability and the great potential that proteomics may have towards forensic sciences.
AB - Current methods for the evaluation of post-mortem interval (PMI) of skeletal remains suffer from poor accuracy, due to the great number of variables that affect the diagenetic process and to the lack of specific guidelines to address this issue. During decomposition, proteins can undergo cumulative decay over the time, resulting in a decrease in the range and abundance of proteins present (i.e., the proteome) in different tissues as well as in an increase of post-translational modifications occurring on these proteins. In this study, we evaluate the applicability of bone proteomic analyses to simulated forensic contexts, looking for specific biomarkers that may help the estimation of PMI and evaluating a previously discovered marker for the estimation of biological age. We noticed a reduction of specific plasma and muscle proteins with increasing PMIs, as well as an increased deamidation of biglycan, a protein with a role in modulating bone growth and mineralization, which may be developed further for the estimation of PMI from a molecular perspective. We also corroborated our previous results regarding the use of fetuin-A as a potential biomarker for the estimation of age-at-death, demonstrating the applicability and the great potential that proteomics may have towards forensic sciences.
KW - Bone proteomics
KW - Forensic proteomics
KW - Post-mortem interval
KW - Biglycan deamidation
KW - Post-mortem interval
UR - https://www.journals.elsevier.com/journal-of-proteomics
UR - http://www.scopus.com/inward/record.url?scp=85044374979&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2018.01.016
DO - 10.1016/j.jprot.2018.01.016
M3 - Article
VL - 177
SP - 21
EP - 30
JO - Journal of Proteomics
T2 - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
ER -