Forensic proteomics for the evaluation of the post-mortem decay in bones

Noemi Procopio, Anna Williams, Andrew T. Chamberlain, Michael Buckley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Current methods for the evaluation of post-mortem interval (PMI) of skeletal remains suffer from poor accuracy, due to the great number of variables that affect the diagenetic process and to the lack of specific guidelines to address this issue. During decomposition, proteins can undergo cumulative decay over the time, resulting in a decrease in the range and abundance of proteins present (i.e., the proteome) in different tissues as well as in an increase of post-translational modifications occurring on these proteins. In this study, we evaluate the applicability of bone proteomic analyses to simulated forensic contexts, looking for specific biomarkers that may help the estimation of PMI and evaluating a previously discovered marker for the estimation of biological age. We noticed a reduction of specific plasma and muscle proteins with increasing PMIs, as well as an increased deamidation of biglycan, a protein with a role in modulating bone growth and mineralization, which may be developed further for the estimation of PMI from a molecular perspective. We also corroborated our previous results regarding the use of fetuin-A as a potential biomarker for the estimation of age-at-death, demonstrating the applicability and the great potential that proteomics may have towards forensic sciences.
Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalJournal of Proteomics
Volume177
Early online date31 Jan 2018
DOIs
Publication statusPublished - 15 Apr 2018

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Proteomics
Bone
Bone and Bones
Biomarkers
Proteins
alpha-2-HS-Glycoprotein
Biglycan
Forensic Sciences
Physiologic Calcification
Muscle Proteins
Bone Development
Proteome
Post Translational Protein Processing
Blood Proteins
Guidelines
Tissue
Decomposition

Cite this

Procopio, Noemi ; Williams, Anna ; Chamberlain, Andrew T. ; Buckley, Michael. / Forensic proteomics for the evaluation of the post-mortem decay in bones. In: Journal of Proteomics. 2018 ; Vol. 177. pp. 21-30.
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Forensic proteomics for the evaluation of the post-mortem decay in bones. / Procopio, Noemi; Williams, Anna; Chamberlain, Andrew T.; Buckley, Michael.

In: Journal of Proteomics, Vol. 177, 15.04.2018, p. 21-30.

Research output: Contribution to journalArticle

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