FTIR spectroscopy: A new diagnostic tool to aid DNA analysis from heated bone

Jamie Daniel Fredericks, Phil Bennett, Anna Williams, Keith Derek Rogers

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Deoxyribonucleic acid (DNA) extracted from skeletal tissue can be invaluable in genetic profiling applications, as it is often the only source available. Like all forensic samples, skeletal tissue may have been exposed to a variety of environmental insults, including heat. This study has focussed upon characterising changes in the material properties of bone that has been compromised by controlled heat treatments. These changes were then examined in relation to the subsequent success or failure of nuclear DNA (nDNA) amplification, using a range of differently sized amplicons, relevant to alternate profiling strategies. The results presented demonstrate that the ability to amplify nDNA correlates well with particular changes in mineral and organic content of bone. As such, we propose the application of a 'diagnostic triage tool' that can be performed quickly and at low cost on individual bone samples, in order to determine whether nDNA analysis is likely to be a viable option.

Original languageEnglish
Pages (from-to)375-380
Number of pages6
JournalForensic Science International: Genetics
Volume6
Issue number3
Early online date1 Oct 2011
DOIs
Publication statusPublished - May 2012
Externally publishedYes

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