Functional analysis of human and chimpanzee promoters.

Florian Heissig, Johannes Krause, Jaroslaw Bryk, Philipp Khaitovich, Wolfgang Enard, Svante Pääbo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background:

It has long been argued that changes in gene expression may provide an additional and crucial perspective on the evolutionary differences between humans and chimpanzees. To investigate how often expression differences seen in tissues are caused by sequence differences in the proximal promoters, we tested the expression activity in cultured cells of human and chimpanzee promoters from genes that differ in mRNA expression between human and chimpanzee tissues.

Results:

Twelve promoters for which the corresponding gene had been shown to be differentially expressed between humans and chimpanzees in liver or brain were tested. Seven showed a significant difference in activity between the human promoter and the orthologous chimpanzee promoter in at least one of the two cell lines used. However, only three of them showed a difference in the same direction as in the tissues.

Conclusion:

Differences in proximal promoter activity are likely to be common between humans and chimpanzees, but are not linked in a simple fashion to gene-expression levels in tissues. This suggests that several genetic differences between humans and chimpanzees might be responsible for a single expression difference and thus that relevant expression differences between humans and chimpanzees will be difficult to predict from cell culture experiments or DNA sequences.

LanguageEnglish
Article numberR57
Number of pages6
JournalGenome Biology
Volume6
Issue number7
DOIs
Publication statusPublished - 1 Jul 2005
Externally publishedYes

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Pan troglodytes
promoter regions
gene expression
gene
brain
Gene Expression
DNA
analysis
tissue
Human Activities
cultured cells
Genes
Cultured Cells
cell culture
genes
Cell Culture Techniques
experiment
cell lines
nucleotide sequences
Cell Line

Cite this

Heissig, F., Krause, J., Bryk, J., Khaitovich, P., Enard, W., & Pääbo, S. (2005). Functional analysis of human and chimpanzee promoters. Genome Biology, 6(7), [R57]. https://doi.org/10.1186/gb-2005-6-7-r57
Heissig, Florian ; Krause, Johannes ; Bryk, Jaroslaw ; Khaitovich, Philipp ; Enard, Wolfgang ; Pääbo, Svante. / Functional analysis of human and chimpanzee promoters. In: Genome Biology. 2005 ; Vol. 6, No. 7.
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Heissig, F, Krause, J, Bryk, J, Khaitovich, P, Enard, W & Pääbo, S 2005, 'Functional analysis of human and chimpanzee promoters.', Genome Biology, vol. 6, no. 7, R57. https://doi.org/10.1186/gb-2005-6-7-r57

Functional analysis of human and chimpanzee promoters. / Heissig, Florian; Krause, Johannes; Bryk, Jaroslaw; Khaitovich, Philipp; Enard, Wolfgang; Pääbo, Svante.

In: Genome Biology, Vol. 6, No. 7, R57, 01.07.2005.

Research output: Contribution to journalArticle

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Heissig F, Krause J, Bryk J, Khaitovich P, Enard W, Pääbo S. Functional analysis of human and chimpanzee promoters. Genome Biology. 2005 Jul 1;6(7). R57. https://doi.org/10.1186/gb-2005-6-7-r57