Functional and immunochemical comparison of hepatic UDP-glucuronosyltransferases in a piscine and a mammalian species

Douglas J. Clarke, Brian Burchell, Stephen G. George

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

1. 1. The aglycone specificity of hepatic microsomal glucuronidation was compared under uniform conditions in a fish, Pleuronectes platessa and a mammal, Rattus norvegicys, representative of the most primitive and advanced vertebrate classes. 2. 2. Both species exhibited comparable UDP-glucuronosyltransferase (UDPGT) activity towards planar phenolic substrates (1-napthol, 4-nitrophenol); however, plaice activity towards bulky non-planar substrates such as (-)-morphine was either 200-fold lower, or for arylacetic acid (RS-2-phenylpropionic acid) and an aryloxyacetic acid (clofibric acid) non-detectable. 3. 3. Conjugation of the endogenous substrates, bilirubin and steroids were 4- to 40-fold lower in the plaice than in the rat. Whilst both species formed diglucuronides of the asymmetrical bilirubin IXα, they displayed a reciprocal preference for the initial esterification, conjugation of the C-8 side chain predominating in the rat and of C-12 in the fish. 4. 4. Immunoblot analysis using two polyclonal antisera preparations raised against rat UDPGTs demonstrated the presence of multiple weakly cross-reacting polypeptides in fish microsomes indicative of multiple isoforms and conservation of common structural motifs over more than 350 million years since evolutionary divergence of the mammals.

LanguageEnglish
Pages425-432
Number of pages8
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume102
Issue number2
DOIs
Publication statusPublished - 1 Jun 1992
Externally publishedYes

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Glucuronosyltransferase
Flounder
Fish
Rats
Mammals
Bilirubin
Fishes
Liver
Substrates
Clofibric Acid
Esterification
Morphine
Acids
Immune Sera
Conservation
Protein Isoforms
Microsomes
Steroids
Vertebrates
Peptides

Cite this

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abstract = "1. 1. The aglycone specificity of hepatic microsomal glucuronidation was compared under uniform conditions in a fish, Pleuronectes platessa and a mammal, Rattus norvegicys, representative of the most primitive and advanced vertebrate classes. 2. 2. Both species exhibited comparable UDP-glucuronosyltransferase (UDPGT) activity towards planar phenolic substrates (1-napthol, 4-nitrophenol); however, plaice activity towards bulky non-planar substrates such as (-)-morphine was either 200-fold lower, or for arylacetic acid (RS-2-phenylpropionic acid) and an aryloxyacetic acid (clofibric acid) non-detectable. 3. 3. Conjugation of the endogenous substrates, bilirubin and steroids were 4- to 40-fold lower in the plaice than in the rat. Whilst both species formed diglucuronides of the asymmetrical bilirubin IXα, they displayed a reciprocal preference for the initial esterification, conjugation of the C-8 side chain predominating in the rat and of C-12 in the fish. 4. 4. Immunoblot analysis using two polyclonal antisera preparations raised against rat UDPGTs demonstrated the presence of multiple weakly cross-reacting polypeptides in fish microsomes indicative of multiple isoforms and conservation of common structural motifs over more than 350 million years since evolutionary divergence of the mammals.",
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Functional and immunochemical comparison of hepatic UDP-glucuronosyltransferases in a piscine and a mammalian species. / Clarke, Douglas J.; Burchell, Brian; George, Stephen G.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 102, No. 2, 01.06.1992, p. 425-432.

Research output: Contribution to journalArticle

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