G-Quadruplex Binding of an NIR Emitting Osmium Polypyridyl Probe Revealed by Solution NMR and Time-Resolved Infrared Studies

Kateřina Peterková; Mark Stitch; Rayhaan Boota; Paul Scattergood; Paul Elliott; Michael Towrie; Peter Podbevšek; Janez Plavec; Susan Quinn

doi:10.1002/chem.202203250

G-Quadruplex Binding of an NIR Emitting Osmium Polypyridyl Probe Revealed by Solution NMR and Time-Resolved Infrared Studies

Kateřina Peterková, Mark Stitch, Rayhaan Boota, Paul Scattergood, Paul Elliott, Michael Towrie, Peter Podbevšek, Janez Plavec, Susan Quinn

Research output: Contribution to journal › Article › peer-review

Abstract

G-quadruplexes are emerging targets in cancer research and understanding how diagnostic probes bind to DNA G-quadruplexes in solution is critical to the development of new molecular tools. In this study the binding of an enantiopure NIR emitting [Os(TAP) ₂(dppz)] ²⁺ complex to different G-quadruplex structures formed by human telomer (hTel) and cMYC sequences in solution is reported. The combination of NMR and time-resolved infrared spectroscopic techniques reveals the sensitivity of the emission response to subtle changes in the binding environment of the complex. Similar behaviour is also observed for the related complex [Os(TAP) ₂(dppp2)] ²⁺ upon quadruplex binding.

Original language	English
Article number	e202203250
Number of pages	7
Journal	Chemistry - A European Journal
Volume	29
Issue number	11
Early online date	12 Jan 2023
DOIs	https://doi.org/10.1002/chem.202203250
Publication status	Published - 21 Feb 2023

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@article{56c83596b5d44434b9e40cb5e6b23f51,

title = "G-Quadruplex Binding of an NIR Emitting Osmium Polypyridyl Probe Revealed by Solution NMR and Time-Resolved Infrared Studies",

abstract = "G-quadruplexes are emerging targets in cancer research and understanding how diagnostic probes bind to DNA G-quadruplexes in solution is critical to the development of new molecular tools. In this study the binding of an enantiopure NIR emitting [Os(TAP) 2(dppz)] 2+ complex to different G-quadruplex structures formed by human telomer (hTel) and cMYC sequences in solution is reported. The combination of NMR and time-resolved infrared spectroscopic techniques reveals the sensitivity of the emission response to subtle changes in the binding environment of the complex. Similar behaviour is also observed for the related complex [Os(TAP) 2(dppp2)] 2+ upon quadruplex binding.",

keywords = "G-quadruplex, NMR, luminescence, DNA-Binding, Infrared, TRIR, spectroscopy, osmium polypyridyl, luminescent enhancement",

author = "Kate{\v r}ina Peterkov{\'a} and Mark Stitch and Rayhaan Boota and Paul Scattergood and Paul Elliott and Michael Towrie and Peter Podbev{\v s}ek and Janez Plavec and Susan Quinn",

note = "Funding Information: The authors would like to acknowledge the following for financial support from the European Union's Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant agreement no 765266 (LightDyNAmics). R.Z.B. acknowledges the University of Huddersfield for the funding of a PhD studentship. K.P., P.P. and J.P. acknowledge financial support from the Slovenian Research Agency [grants P1-0242 and J1-1704]. The authors acknowledge the CERIC-ERIC Consortium for the access to experimental facilities. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement no. 871124 Laserlab-Europe (Central Laser Facility, STFC ID 22130030). Open Access funding provided by IReL. Funding Information: The authors would like to acknowledge the following for financial support from the European Union's Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska‐Curie grant agreement no 765266 (LightDyNAmics). R.Z.B. acknowledges the University of Huddersfield for the funding of a PhD studentship. K.P., P.P. and J.P. acknowledge financial support from the Slovenian Research Agency [grants P1‐0242 and J1‐1704]. The authors acknowledge the CERIC‐ERIC Consortium for the access to experimental facilities. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement no. 871124 Laserlab‐Europe (Central Laser Facility, STFC ID 22130030). Open Access funding provided by IReL. Publisher Copyright: {\textcopyright} 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.",

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doi = "10.1002/chem.202203250",

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AU - Peterková, Kateřina

AU - Stitch, Mark

AU - Boota, Rayhaan

AU - Scattergood, Paul

AU - Elliott, Paul

AU - Towrie, Michael

AU - Podbevšek, Peter

AU - Plavec, Janez

AU - Quinn, Susan

N1 - Funding Information: The authors would like to acknowledge the following for financial support from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 765266 (LightDyNAmics). R.Z.B. acknowledges the University of Huddersfield for the funding of a PhD studentship. K.P., P.P. and J.P. acknowledge financial support from the Slovenian Research Agency [grants P1-0242 and J1-1704]. The authors acknowledge the CERIC-ERIC Consortium for the access to experimental facilities. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement no. 871124 Laserlab-Europe (Central Laser Facility, STFC ID 22130030). Open Access funding provided by IReL. Funding Information: The authors would like to acknowledge the following for financial support from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska‐Curie grant agreement no 765266 (LightDyNAmics). R.Z.B. acknowledges the University of Huddersfield for the funding of a PhD studentship. K.P., P.P. and J.P. acknowledge financial support from the Slovenian Research Agency [grants P1‐0242 and J1‐1704]. The authors acknowledge the CERIC‐ERIC Consortium for the access to experimental facilities. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement no. 871124 Laserlab‐Europe (Central Laser Facility, STFC ID 22130030). Open Access funding provided by IReL. Publisher Copyright: © 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.

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N2 - G-quadruplexes are emerging targets in cancer research and understanding how diagnostic probes bind to DNA G-quadruplexes in solution is critical to the development of new molecular tools. In this study the binding of an enantiopure NIR emitting [Os(TAP) 2(dppz)] 2+ complex to different G-quadruplex structures formed by human telomer (hTel) and cMYC sequences in solution is reported. The combination of NMR and time-resolved infrared spectroscopic techniques reveals the sensitivity of the emission response to subtle changes in the binding environment of the complex. Similar behaviour is also observed for the related complex [Os(TAP) 2(dppp2)] 2+ upon quadruplex binding.

AB - G-quadruplexes are emerging targets in cancer research and understanding how diagnostic probes bind to DNA G-quadruplexes in solution is critical to the development of new molecular tools. In this study the binding of an enantiopure NIR emitting [Os(TAP) 2(dppz)] 2+ complex to different G-quadruplex structures formed by human telomer (hTel) and cMYC sequences in solution is reported. The combination of NMR and time-resolved infrared spectroscopic techniques reveals the sensitivity of the emission response to subtle changes in the binding environment of the complex. Similar behaviour is also observed for the related complex [Os(TAP) 2(dppp2)] 2+ upon quadruplex binding.

KW - G-quadruplex

KW - NMR

KW - luminescence

KW - DNA-Binding

KW - Infrared

KW - TRIR

KW - spectroscopy

KW - osmium polypyridyl

KW - luminescent enhancement

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U2 - 10.1002/chem.202203250

DO - 10.1002/chem.202203250

M3 - Article

VL - 29

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

SN - 0947-6539

IS - 11

M1 - e202203250

ER -

G-Quadruplex Binding of an NIR Emitting Osmium Polypyridyl Probe Revealed by Solution NMR and Time-Resolved Infrared Studies

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University College Dublin

NMR titration experiments that study binding of a NIR emitting osmium polypyridyl probe to cMYC and hTel G-quadruplex DNA

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G-Quadruplex Binding of an NIR Emitting Osmium Polypyridyl Probe Revealed by Solution NMR and Time-Resolved Infrared Studies

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NMR titration experiments that study binding of a NIR emitting osmium polypyridyl probe to cMYC and hTel G-quadruplex DNA

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