Genomic Insights Into A Novel, Alkalitolerant Nitrogen Fixing Bacteria, Azonexus sp. Strain ZS02.

Zohier Salah, Christopher Charles, Paul Humphreys, Andrew Laws, Simon Rout

Research output: Contribution to journalArticle

Abstract

Alkaline environments represent a significant challenge to the growth of micro-organisms. Despite this, there are a number of alkaline environments which contain active microbial communities. Here we describe the genome of a diazotrophic, alkalitolerant strain of Azonexus, which was isolated from a microcosm seeded with hyperalkaline soils resulting from lime depositions. The isolate has a genome size 3.60 Mb with 3431 protein coding genes. The
proteome indicated the presence of genes associated with the cycling of nitrogen, in particular the fixation of atmospheric nitrogen. Although closely related to Azonexus hydrophilus strain d8-1 by both 16S (97.9%) and in silico gDNA (84.1%) relatedness, the isolate demonstrates a pH tolerance above that reported for this strain. The proteome contained genes for the complete
Na+ /H+ antiporter (subunits A to G) for cytoplasmic pH regulation; this may account for the phenotypic characteristics of this strain which exhibited optimal growth conditions of pH 9 and 30°C.
LanguageEnglish
Number of pages6
JournalJournal of Genomics
Volume7
Early online dateNov 2018
DOIs
Publication statusPublished - 1 Jan 2019

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Bacteria
Nitrogen
Genes
Antiporters
Genome Size
Nitrogen Fixation
Proteome
Growth
Computer Simulation
Soil
Genome
Nitrogen-Fixing Bacteria
Proteins
Soils
lime

Cite this

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abstract = "Alkaline environments represent a significant challenge to the growth of micro-organisms. Despite this, there are a number of alkaline environments which contain active microbial communities. Here we describe the genome of a diazotrophic, alkalitolerant strain of Azonexus, which was isolated from a microcosm seeded with hyperalkaline soils resulting from lime depositions. The isolate has a genome size 3.60 Mb with 3431 protein coding genes. Theproteome indicated the presence of genes associated with the cycling of nitrogen, in particular the fixation of atmospheric nitrogen. Although closely related to Azonexus hydrophilus strain d8-1 by both 16S (97.9{\%}) and in silico gDNA (84.1{\%}) relatedness, the isolate demonstrates a pH tolerance above that reported for this strain. The proteome contained genes for the completeNa+ /H+ antiporter (subunits A to G) for cytoplasmic pH regulation; this may account for the phenotypic characteristics of this strain which exhibited optimal growth conditions of pH 9 and 30°C.",
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Genomic Insights Into A Novel, Alkalitolerant Nitrogen Fixing Bacteria, Azonexus sp. Strain ZS02. / Salah, Zohier; Charles, Christopher; Humphreys, Paul; Laws, Andrew; Rout, Simon.

In: Journal of Genomics, Vol. 7, 01.01.2019.

Research output: Contribution to journalArticle

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