Global conformation analysis of irradiated xyloglucans

Trushar R. Patel, Gordon A. Morris, Anna Ebringerová, Melita Vodeničarová, Vladimír Velebný, Alvaro Ortega, Jose Garcia de la Torre, Stephen E. Harding

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Xyloglucan isolated and purified from tamarind seed was subjected to various degrees of γ-irradiation treatments, from 10 to 70 kGy, monitored for radiation damage and then studied using a new combined hydrodynamic approach with regards to conformation and flexibility. Radiation products were analysed with regard to molecular weight (weight average) Mw from size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLs), intrinsic viscosity [η] and sedimentation coefficient so20,w. Sedimentation coefficient distributions and elution profiles from SEC-MALLs confirmed the unimodal nature of the molecular weight distribution for each sample in solution. The chain flexibility was then investigated in terms of the persistence length, Lp of the equivalent worm-like chain model. The traditional Bushin-Bohdanecky (intrinsic viscosity) and Yamakawa-Fujii (sedimentation coefficient) relations were used separately then combined together by minimisation of a target function according to a recently published procedure [Ortega, A., & García de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475 [see also Ortega, A. Metodologías computacionales para propiedades en disolución de macromoléculas rígidas y flexibles. Ph.D. Dissertation, Universidad de Murcia, 2005]] and yielded an estimate for Lp in the range 4-9 nm using floated and fixed mass per unit length analysis protocols and "point" global analysis: irradiated xyloglucans behave as flexible structures in common with pressure/heat treated materials.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalCarbohydrate Polymers
Volume74
Issue number4
Early online date15 May 2008
DOIs
Publication statusPublished - 21 Nov 2008
Externally publishedYes

Fingerprint

Sedimentation
Conformations
Viscosity
Flexible structures
Size exclusion chromatography
Radiation damage
Molecular weight distribution
Light scattering
Seed
Hydrodynamics
Molecular weight
Irradiation
Radiation
Lasers
xyloglucan

Cite this

Patel, T. R., Morris, G. A., Ebringerová, A., Vodeničarová, M., Velebný, V., Ortega, A., ... Harding, S. E. (2008). Global conformation analysis of irradiated xyloglucans. Carbohydrate Polymers, 74(4), 845-851. https://doi.org/10.1016/j.carbpol.2008.05.006
Patel, Trushar R. ; Morris, Gordon A. ; Ebringerová, Anna ; Vodeničarová, Melita ; Velebný, Vladimír ; Ortega, Alvaro ; Garcia de la Torre, Jose ; Harding, Stephen E. / Global conformation analysis of irradiated xyloglucans. In: Carbohydrate Polymers. 2008 ; Vol. 74, No. 4. pp. 845-851.
@article{8332dd69bee74148a0a422484539869a,
title = "Global conformation analysis of irradiated xyloglucans",
abstract = "Xyloglucan isolated and purified from tamarind seed was subjected to various degrees of γ-irradiation treatments, from 10 to 70 kGy, monitored for radiation damage and then studied using a new combined hydrodynamic approach with regards to conformation and flexibility. Radiation products were analysed with regard to molecular weight (weight average) Mw from size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLs), intrinsic viscosity [η] and sedimentation coefficient so20,w. Sedimentation coefficient distributions and elution profiles from SEC-MALLs confirmed the unimodal nature of the molecular weight distribution for each sample in solution. The chain flexibility was then investigated in terms of the persistence length, Lp of the equivalent worm-like chain model. The traditional Bushin-Bohdanecky (intrinsic viscosity) and Yamakawa-Fujii (sedimentation coefficient) relations were used separately then combined together by minimisation of a target function according to a recently published procedure [Ortega, A., & Garc{\'i}a de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475 [see also Ortega, A. Metodolog{\'i}as computacionales para propiedades en disoluci{\'o}n de macromol{\'e}culas r{\'i}gidas y flexibles. Ph.D. Dissertation, Universidad de Murcia, 2005]] and yielded an estimate for Lp in the range 4-9 nm using floated and fixed mass per unit length analysis protocols and {"}point{"} global analysis: irradiated xyloglucans behave as flexible structures in common with pressure/heat treated materials.",
keywords = "γ-irradiation, Bushin-Bohdanecky, Chain flexibility, Combined {"}global{"} analysis, Xyloglucan, Yamakawa-Fujii",
author = "Patel, {Trushar R.} and Morris, {Gordon A.} and Anna Ebringerov{\'a} and Melita Vodeničarov{\'a} and Vladim{\'i}r Velebn{\'y} and Alvaro Ortega and {Garcia de la Torre}, Jose and Harding, {Stephen E.}",
year = "2008",
month = "11",
day = "21",
doi = "10.1016/j.carbpol.2008.05.006",
language = "English",
volume = "74",
pages = "845--851",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",
number = "4",

}

Patel, TR, Morris, GA, Ebringerová, A, Vodeničarová, M, Velebný, V, Ortega, A, Garcia de la Torre, J & Harding, SE 2008, 'Global conformation analysis of irradiated xyloglucans', Carbohydrate Polymers, vol. 74, no. 4, pp. 845-851. https://doi.org/10.1016/j.carbpol.2008.05.006

Global conformation analysis of irradiated xyloglucans. / Patel, Trushar R.; Morris, Gordon A.; Ebringerová, Anna; Vodeničarová, Melita; Velebný, Vladimír; Ortega, Alvaro; Garcia de la Torre, Jose; Harding, Stephen E.

In: Carbohydrate Polymers, Vol. 74, No. 4, 21.11.2008, p. 845-851.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Global conformation analysis of irradiated xyloglucans

AU - Patel, Trushar R.

AU - Morris, Gordon A.

AU - Ebringerová, Anna

AU - Vodeničarová, Melita

AU - Velebný, Vladimír

AU - Ortega, Alvaro

AU - Garcia de la Torre, Jose

AU - Harding, Stephen E.

PY - 2008/11/21

Y1 - 2008/11/21

N2 - Xyloglucan isolated and purified from tamarind seed was subjected to various degrees of γ-irradiation treatments, from 10 to 70 kGy, monitored for radiation damage and then studied using a new combined hydrodynamic approach with regards to conformation and flexibility. Radiation products were analysed with regard to molecular weight (weight average) Mw from size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLs), intrinsic viscosity [η] and sedimentation coefficient so20,w. Sedimentation coefficient distributions and elution profiles from SEC-MALLs confirmed the unimodal nature of the molecular weight distribution for each sample in solution. The chain flexibility was then investigated in terms of the persistence length, Lp of the equivalent worm-like chain model. The traditional Bushin-Bohdanecky (intrinsic viscosity) and Yamakawa-Fujii (sedimentation coefficient) relations were used separately then combined together by minimisation of a target function according to a recently published procedure [Ortega, A., & García de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475 [see also Ortega, A. Metodologías computacionales para propiedades en disolución de macromoléculas rígidas y flexibles. Ph.D. Dissertation, Universidad de Murcia, 2005]] and yielded an estimate for Lp in the range 4-9 nm using floated and fixed mass per unit length analysis protocols and "point" global analysis: irradiated xyloglucans behave as flexible structures in common with pressure/heat treated materials.

AB - Xyloglucan isolated and purified from tamarind seed was subjected to various degrees of γ-irradiation treatments, from 10 to 70 kGy, monitored for radiation damage and then studied using a new combined hydrodynamic approach with regards to conformation and flexibility. Radiation products were analysed with regard to molecular weight (weight average) Mw from size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLs), intrinsic viscosity [η] and sedimentation coefficient so20,w. Sedimentation coefficient distributions and elution profiles from SEC-MALLs confirmed the unimodal nature of the molecular weight distribution for each sample in solution. The chain flexibility was then investigated in terms of the persistence length, Lp of the equivalent worm-like chain model. The traditional Bushin-Bohdanecky (intrinsic viscosity) and Yamakawa-Fujii (sedimentation coefficient) relations were used separately then combined together by minimisation of a target function according to a recently published procedure [Ortega, A., & García de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475 [see also Ortega, A. Metodologías computacionales para propiedades en disolución de macromoléculas rígidas y flexibles. Ph.D. Dissertation, Universidad de Murcia, 2005]] and yielded an estimate for Lp in the range 4-9 nm using floated and fixed mass per unit length analysis protocols and "point" global analysis: irradiated xyloglucans behave as flexible structures in common with pressure/heat treated materials.

KW - γ-irradiation

KW - Bushin-Bohdanecky

KW - Chain flexibility

KW - Combined "global" analysis

KW - Xyloglucan

KW - Yamakawa-Fujii

UR - http://www.scopus.com/inward/record.url?scp=49949094775&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2008.05.006

DO - 10.1016/j.carbpol.2008.05.006

M3 - Article

VL - 74

SP - 845

EP - 851

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 4

ER -

Patel TR, Morris GA, Ebringerová A, Vodeničarová M, Velebný V, Ortega A et al. Global conformation analysis of irradiated xyloglucans. Carbohydrate Polymers. 2008 Nov 21;74(4):845-851. https://doi.org/10.1016/j.carbpol.2008.05.006