Global hydrodynamic analysis of the molecular flexibility of galactomannans

Gordon A. Morris, Trushar R. Patel, David R. Picout, Simon B. Ross-Murphy, Alvaro Ortega, Jose Garcia de la Torre, Stephen E. Harding

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In the past, intrinsic viscosity and sedimentation velocity analyses have been used separately to assess the conformation and flexibility of guar and locust bean gum galactomannans based on worm-like chain and semi-flexible coil models. Publication of a new global method combining data sets of both intrinsic viscosity and sedimentation coefficient with molecular weight, and minimising a target (error) function now permits a more robust analysis. Using this approach, values for the persistence length of (10 ± 2) nm for guar and (7 ± 1) nm for locust bean gum are returned if the mass per unit length ML is floated as a variable. Using a fixed mass per unit length based on the known compositional data of each galactomannan yields a similar value for Lp in both cases, (8 ± 1) nm for guar and (9 ± 1) nm for locust bean gum, with combined set of data yielding (9 ± 1) nm: within experimental error the flexibilities of both galactomannans are very similar.

Original languageEnglish
Pages (from-to)356-360
Number of pages5
JournalCarbohydrate Polymers
Volume72
Issue number2
Early online date29 Aug 2007
DOIs
Publication statusPublished - 5 May 2008
Externally publishedYes

Fingerprint Dive into the research topics of 'Global hydrodynamic analysis of the molecular flexibility of galactomannans'. Together they form a unique fingerprint.

  • Cite this

    Morris, G. A., Patel, T. R., Picout, D. R., Ross-Murphy, S. B., Ortega, A., Garcia de la Torre, J., & Harding, S. E. (2008). Global hydrodynamic analysis of the molecular flexibility of galactomannans. Carbohydrate Polymers, 72(2), 356-360. https://doi.org/10.1016/j.carbpol.2007.08.017