Gluconeogenesis, glucose handling, and structural changes in livers of the adult offspring of rats partially deprived of protein during pregnancy and lactation

Shamus P. Burns, Mina Desai, Robert D. Cohen, C. Nicholas Hales, Richard A. Iles, Jocelyn P. Germain, Thomas C.H. Going, Rosemary A. Bailey

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Maternal protein restriction is a model of fetal programming of adult glucose intolerance. Perfused livers of 48-h-starved adult offspring of rat dams fed 8% protein diets during pregnancy and lactation produced more glucose from 6 mM lactate than did control livers from rats whose dams were fed 20% protein. In control livers, a mean of 24% of the glucose formed from lactate in the periportal region of the lobule was taken up by the most distal perivenous cells; this distal perivenous uptake was greatly diminished in maternal low protein (MLP) livers, accounting for a major fraction of the increased glucose output of MLP livers. In control livers, the distal perivenous cells contained 40% of the total glucokinase of the liver; this perivenous concentration of glucokinase was greatly reduced in MLP livers. Intralobular distribution of phosphenolpyruvate carboxykinase was unaltered, though overall increased activity could have contributed to the elevated glucose output. Hepatic lobular volume in MLP livers was twice that in control livers, indicating that MLP livers had half the normal number of lobules. Fetal programming of adult glucose metabolism may operate partly through structural alterations and changes in glucokinase expression in the immediate perivenous region.
LanguageEnglish
Pages1768-1774
Number of pages7
JournalJournal of Clinical Investigation
Volume100
Issue number7
DOIs
Publication statusPublished - 1 Oct 1997
Externally publishedYes

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Pregnancy Proteins
Gluconeogenesis
Lactation
Glucose
Liver
Mothers
Glucokinase
Proteins
Fetal Development
Lactic Acid
Glucose Intolerance

Cite this

Burns, Shamus P. ; Desai, Mina ; Cohen, Robert D. ; Hales, C. Nicholas ; Iles, Richard A. ; Germain, Jocelyn P. ; Going, Thomas C.H. ; Bailey, Rosemary A. / Gluconeogenesis, glucose handling, and structural changes in livers of the adult offspring of rats partially deprived of protein during pregnancy and lactation. In: Journal of Clinical Investigation. 1997 ; Vol. 100, No. 7. pp. 1768-1774.
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Gluconeogenesis, glucose handling, and structural changes in livers of the adult offspring of rats partially deprived of protein during pregnancy and lactation. / Burns, Shamus P.; Desai, Mina; Cohen, Robert D.; Hales, C. Nicholas; Iles, Richard A.; Germain, Jocelyn P.; Going, Thomas C.H.; Bailey, Rosemary A.

In: Journal of Clinical Investigation, Vol. 100, No. 7, 01.10.1997, p. 1768-1774.

Research output: Contribution to journalArticle

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AU - Burns, Shamus P.

AU - Desai, Mina

AU - Cohen, Robert D.

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AU - Iles, Richard A.

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