Heparin-binding Growth-associated Molecule Contains Two Heparin-binding β-Sheet Domains That Are Homologous to the Thrombospondin Type I Repeat

Ilkka Kilpeläinen, Marko Kaksonen, Tarja Kinnunen, Hanna Avikainen, Melissa Fath, Robert J. Linhardt, Erkki Raulo, Heikki Rauvala

Research output: Contribution to journalArticle

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Abstract

Heparin-binding growth-associated molecule (HB-GAM) is an extracellular matrix-associated protein implicated in the development and plasticity of neuronal connections of brain. Binding to cell surface heparan sulfate is indispensable for the biological activity of HB-GAM. In the present paper we have studied the structure of recombinant HB-GAM using heteronuclear MR. These studies show that HB-GAM contains two β-sheet domains connected by a flexible linker. Both of these domains contain three antiparallel β-strands. In addition to this domain structure, HB-GAM contains the N- and C-terminal lysine-rich sequences that lack a detectable structure and appear to form random coils. Studies using CD and MR spectroscopy suggest that HB-GAM undergoes a conformational change upon binding to heparin, and that the binding occurs primarily to the β-sheet domains of the protein. Search of sequence data bases shows that the β-sheet domains of HB-GAM are homologous to the thrombospondin type I repeat (TSR). Sequence comparisons show that the β-sheet structures found previously in midline, a protein homologous with HB-GAM, also correspond to the TSR motif. We suggest that the TSR sequence motif found in various extracellular proteins defines a β-sheet structure similar to that found in HB-GAM and midline. In addition to the apparent structural similarity, a similarity in biological functions is suggested by the occurrence of the TSR sequence motif in a wide variety of proteins that mediate cell-to-extracellular matrix and cell-to-cell interactions, in which the TSR domain mediates specific cell surface binding.

Original languageEnglish
Pages (from-to)13564-13570
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number18
DOIs
Publication statusPublished - 5 May 2000
Externally publishedYes

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Thrombospondins
Heparin
Proteins
pleiotrophin
Neuronal Plasticity
Heparitin Sulfate
Extracellular Matrix Proteins
Bioactivity
Cell Communication
Lysine
Extracellular Matrix
Plasticity
Brain
Magnetic Resonance Spectroscopy
Spectroscopy
Databases

Cite this

Kilpeläinen, Ilkka ; Kaksonen, Marko ; Kinnunen, Tarja ; Avikainen, Hanna ; Fath, Melissa ; Linhardt, Robert J. ; Raulo, Erkki ; Rauvala, Heikki. / Heparin-binding Growth-associated Molecule Contains Two Heparin-binding β-Sheet Domains That Are Homologous to the Thrombospondin Type I Repeat. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 18. pp. 13564-13570.
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abstract = "Heparin-binding growth-associated molecule (HB-GAM) is an extracellular matrix-associated protein implicated in the development and plasticity of neuronal connections of brain. Binding to cell surface heparan sulfate is indispensable for the biological activity of HB-GAM. In the present paper we have studied the structure of recombinant HB-GAM using heteronuclear MR. These studies show that HB-GAM contains two β-sheet domains connected by a flexible linker. Both of these domains contain three antiparallel β-strands. In addition to this domain structure, HB-GAM contains the N- and C-terminal lysine-rich sequences that lack a detectable structure and appear to form random coils. Studies using CD and MR spectroscopy suggest that HB-GAM undergoes a conformational change upon binding to heparin, and that the binding occurs primarily to the β-sheet domains of the protein. Search of sequence data bases shows that the β-sheet domains of HB-GAM are homologous to the thrombospondin type I repeat (TSR). Sequence comparisons show that the β-sheet structures found previously in midline, a protein homologous with HB-GAM, also correspond to the TSR motif. We suggest that the TSR sequence motif found in various extracellular proteins defines a β-sheet structure similar to that found in HB-GAM and midline. In addition to the apparent structural similarity, a similarity in biological functions is suggested by the occurrence of the TSR sequence motif in a wide variety of proteins that mediate cell-to-extracellular matrix and cell-to-cell interactions, in which the TSR domain mediates specific cell surface binding.",
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Heparin-binding Growth-associated Molecule Contains Two Heparin-binding β-Sheet Domains That Are Homologous to the Thrombospondin Type I Repeat. / Kilpeläinen, Ilkka; Kaksonen, Marko; Kinnunen, Tarja; Avikainen, Hanna; Fath, Melissa; Linhardt, Robert J.; Raulo, Erkki; Rauvala, Heikki.

In: Journal of Biological Chemistry, Vol. 275, No. 18, 05.05.2000, p. 13564-13570.

Research output: Contribution to journalArticle

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T1 - Heparin-binding Growth-associated Molecule Contains Two Heparin-binding β-Sheet Domains That Are Homologous to the Thrombospondin Type I Repeat

AU - Kilpeläinen, Ilkka

AU - Kaksonen, Marko

AU - Kinnunen, Tarja

AU - Avikainen, Hanna

AU - Fath, Melissa

AU - Linhardt, Robert J.

AU - Raulo, Erkki

AU - Rauvala, Heikki

PY - 2000/5/5

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N2 - Heparin-binding growth-associated molecule (HB-GAM) is an extracellular matrix-associated protein implicated in the development and plasticity of neuronal connections of brain. Binding to cell surface heparan sulfate is indispensable for the biological activity of HB-GAM. In the present paper we have studied the structure of recombinant HB-GAM using heteronuclear MR. These studies show that HB-GAM contains two β-sheet domains connected by a flexible linker. Both of these domains contain three antiparallel β-strands. In addition to this domain structure, HB-GAM contains the N- and C-terminal lysine-rich sequences that lack a detectable structure and appear to form random coils. Studies using CD and MR spectroscopy suggest that HB-GAM undergoes a conformational change upon binding to heparin, and that the binding occurs primarily to the β-sheet domains of the protein. Search of sequence data bases shows that the β-sheet domains of HB-GAM are homologous to the thrombospondin type I repeat (TSR). Sequence comparisons show that the β-sheet structures found previously in midline, a protein homologous with HB-GAM, also correspond to the TSR motif. We suggest that the TSR sequence motif found in various extracellular proteins defines a β-sheet structure similar to that found in HB-GAM and midline. In addition to the apparent structural similarity, a similarity in biological functions is suggested by the occurrence of the TSR sequence motif in a wide variety of proteins that mediate cell-to-extracellular matrix and cell-to-cell interactions, in which the TSR domain mediates specific cell surface binding.

AB - Heparin-binding growth-associated molecule (HB-GAM) is an extracellular matrix-associated protein implicated in the development and plasticity of neuronal connections of brain. Binding to cell surface heparan sulfate is indispensable for the biological activity of HB-GAM. In the present paper we have studied the structure of recombinant HB-GAM using heteronuclear MR. These studies show that HB-GAM contains two β-sheet domains connected by a flexible linker. Both of these domains contain three antiparallel β-strands. In addition to this domain structure, HB-GAM contains the N- and C-terminal lysine-rich sequences that lack a detectable structure and appear to form random coils. Studies using CD and MR spectroscopy suggest that HB-GAM undergoes a conformational change upon binding to heparin, and that the binding occurs primarily to the β-sheet domains of the protein. Search of sequence data bases shows that the β-sheet domains of HB-GAM are homologous to the thrombospondin type I repeat (TSR). Sequence comparisons show that the β-sheet structures found previously in midline, a protein homologous with HB-GAM, also correspond to the TSR motif. We suggest that the TSR sequence motif found in various extracellular proteins defines a β-sheet structure similar to that found in HB-GAM and midline. In addition to the apparent structural similarity, a similarity in biological functions is suggested by the occurrence of the TSR sequence motif in a wide variety of proteins that mediate cell-to-extracellular matrix and cell-to-cell interactions, in which the TSR domain mediates specific cell surface binding.

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