Heparin in Acid and Alkaline Environments—A Study of the Correlations between Hydrodynamic Properties and Desulphation

Aleksandra Kozlowski, Vlad Dinu, Thomas MacCalman, Alan Smith, Johannes Roubroeks, Edwin Yates, Stephen Harding, Gordon Morris

Research output: Contribution to journalArticlepeer-review

Abstract

This work evaluated the hydrodynamic properties of heparin hydrolysed at temperatures ranging from 40 °C to 80 °C in buffered acid and alkaline environments. The correlation between hydrodynamic parameters led to the conclusion that polymer conformational changes appeared to be minimal until chain depolymerisation, initiated at pH 1 and 80 °C. However, the synergy of conformational changes, even if minimal, and sulphate loss observed at pH 1, pH 3 and pH 12 (various temperatures) resulted in a loss of the antifactor Xa activity. Therefore, the ‘contribution’ of conformational changes should be added to the generally recognized effect of desulphation towards the activity of heparin. This is of significance as the processing of medical heparin is complex, and requires adjustment of several physical and chemical factors, including pH and temperature.
Original languageEnglish
Pages (from-to)88-98
Number of pages11
JournalPolysaccharides
Volume4
Issue number2
Early online date23 Mar 2023
DOIs
Publication statusPublished - 1 Jun 2023

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