How Serratia marcescens HB-4 absorbs cadmium and its implication on phytoremediation

Yakui Chen, Qifa Zhu, Xiangzhou Dong, Weiwei Huang, Chenyu Du, Diannan Lu

Research output: Contribution to journalArticle

Abstract

A novel strain Serratia marcescens HB-4 with high Cadmium adsorption capacity was isolated from heavy metal contaminated soil in Hunan province, China. S. marcescens HB-4 reduced the concentration of Cd present in wastewater to less than 0.1 mg/L when the inlet stream contained no higher than 5.0 mg/L Cd. After treatment, wastewater meets Integrated Wastewater Discharge Standard of China (GB8978-1996). The naturally dead S. marcescens HB-4 still maintained over 80% of its Cd adsorption capacity. Scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive spectroscopy (EDS) results suggested that the mechanism of Cd adsorption can be explained as the synergy of extracellular adsorption, periplasm accumulation and intracellular absorption. The size of the accumulated Cd particular is at the nanometer scale, which can be washed out by EDTA without damaging cell integrity. SDS-polyacrylamide gel electrophoresis experiment showed that the heavy metal binding protein (especially Fe binding protein), transporter, amino acid and histidine periplasmic binding proteins and oxidoreductases were responsible for Cd removal. The pot experiment of S. marcescens HB-4 combined with Houttuynia cordata to detoxify Cd contaminated soil showed that the cadmium content in the aboveground and underground parts of Houttuynia cordata increased by 34.48% and 59.13% (w/w), respectively. The cadmium accumulation in Houttuynia cordata increased by 44.27% compared with the blank group which was not combined with S. marcescens HB-4. This work demonstrates that microbial synergistic phytoremediation has a significant potential to treat heavy metal contaminated soil.

Original languageEnglish
Article number109723
JournalEcotoxicology and Environmental Safety
Volume185
Early online date3 Oct 2019
DOIs
Publication statusE-pub ahead of print - 3 Oct 2019

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Serratia marcescens
Environmental Biodegradation
Houttuynia
Cadmium
Adsorption
Heavy metals
Waste Water
Heavy Metals
Soils
Soil
Wastewater
Electron microscopes
China
Carrier Proteins
Periplasmic Binding Proteins
Electrons
Ethylenediaminetetraacetic acid
Amino Acid Transport Systems
Polyacrylates
Electrophoresis

Cite this

Chen, Yakui ; Zhu, Qifa ; Dong, Xiangzhou ; Huang, Weiwei ; Du, Chenyu ; Lu, Diannan. / How Serratia marcescens HB-4 absorbs cadmium and its implication on phytoremediation. In: Ecotoxicology and Environmental Safety. 2019 ; Vol. 185.
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abstract = "A novel strain Serratia marcescens HB-4 with high Cadmium adsorption capacity was isolated from heavy metal contaminated soil in Hunan province, China. S. marcescens HB-4 reduced the concentration of Cd present in wastewater to less than 0.1 mg/L when the inlet stream contained no higher than 5.0 mg/L Cd. After treatment, wastewater meets Integrated Wastewater Discharge Standard of China (GB8978-1996). The naturally dead S. marcescens HB-4 still maintained over 80{\%} of its Cd adsorption capacity. Scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive spectroscopy (EDS) results suggested that the mechanism of Cd adsorption can be explained as the synergy of extracellular adsorption, periplasm accumulation and intracellular absorption. The size of the accumulated Cd particular is at the nanometer scale, which can be washed out by EDTA without damaging cell integrity. SDS-polyacrylamide gel electrophoresis experiment showed that the heavy metal binding protein (especially Fe binding protein), transporter, amino acid and histidine periplasmic binding proteins and oxidoreductases were responsible for Cd removal. The pot experiment of S. marcescens HB-4 combined with Houttuynia cordata to detoxify Cd contaminated soil showed that the cadmium content in the aboveground and underground parts of Houttuynia cordata increased by 34.48{\%} and 59.13{\%} (w/w), respectively. The cadmium accumulation in Houttuynia cordata increased by 44.27{\%} compared with the blank group which was not combined with S. marcescens HB-4. This work demonstrates that microbial synergistic phytoremediation has a significant potential to treat heavy metal contaminated soil.",
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How Serratia marcescens HB-4 absorbs cadmium and its implication on phytoremediation. / Chen, Yakui; Zhu, Qifa; Dong, Xiangzhou; Huang, Weiwei; Du, Chenyu; Lu, Diannan.

In: Ecotoxicology and Environmental Safety, Vol. 185, 109723, 15.12.2019.

Research output: Contribution to journalArticle

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T1 - How Serratia marcescens HB-4 absorbs cadmium and its implication on phytoremediation

AU - Chen, Yakui

AU - Zhu, Qifa

AU - Dong, Xiangzhou

AU - Huang, Weiwei

AU - Du, Chenyu

AU - Lu, Diannan

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AB - A novel strain Serratia marcescens HB-4 with high Cadmium adsorption capacity was isolated from heavy metal contaminated soil in Hunan province, China. S. marcescens HB-4 reduced the concentration of Cd present in wastewater to less than 0.1 mg/L when the inlet stream contained no higher than 5.0 mg/L Cd. After treatment, wastewater meets Integrated Wastewater Discharge Standard of China (GB8978-1996). The naturally dead S. marcescens HB-4 still maintained over 80% of its Cd adsorption capacity. Scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive spectroscopy (EDS) results suggested that the mechanism of Cd adsorption can be explained as the synergy of extracellular adsorption, periplasm accumulation and intracellular absorption. The size of the accumulated Cd particular is at the nanometer scale, which can be washed out by EDTA without damaging cell integrity. SDS-polyacrylamide gel electrophoresis experiment showed that the heavy metal binding protein (especially Fe binding protein), transporter, amino acid and histidine periplasmic binding proteins and oxidoreductases were responsible for Cd removal. The pot experiment of S. marcescens HB-4 combined with Houttuynia cordata to detoxify Cd contaminated soil showed that the cadmium content in the aboveground and underground parts of Houttuynia cordata increased by 34.48% and 59.13% (w/w), respectively. The cadmium accumulation in Houttuynia cordata increased by 44.27% compared with the blank group which was not combined with S. marcescens HB-4. This work demonstrates that microbial synergistic phytoremediation has a significant potential to treat heavy metal contaminated soil.

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KW - Microorganism

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