Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: A comparison with xanthan

G. A. Morris, P. Li, M. Puaud, Z. Liu, J. R. Mitchell, S. E. Harding

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A bacterial exopolysaccharide from Aphanothece halophytica GR02 (AH-EPS) solubilized in buffered 1 M guanidine hydrochloride, GuHCl was studied using capillary viscometry, double gap viscometry, sedimentation velocity and sedimentation equilibrium using analytical ultracentrifugation. Results from capillary viscometry indicate that the exopolysaccharide is xanthan-like in its shear thinning properties. This was confirmed by double gap viscometry. Sedimentation velocity (distribution of apparent sedimentation coefficients) confirmed that the AH-EPS solution was reasonably homogeneous and a hyper-sharp boundary indicated that AH-EPS is a rigid/extra-rigid rod type polysaccharide. Sedimentation equilibrium confirmed the high molecular weight indicated by viscosity measurements. In order to ascertain whether the solution physical properties of AH-EPS are xanthan-like under the conditions studied, all the above experiments were repeated on a similarly treated xanthan solution in the same solvent. Results indicate that AH-EPS is indeed xanthan-like in its physical properties at least in the system studied. Xanthan properties in GuHCl do not appear too different from those studied in more traditional solvents.

LanguageEnglish
Pages261-268
Number of pages8
JournalCarbohydrate Polymers
Volume44
Issue number3
Early online date27 Nov 2000
DOIs
Publication statusPublished - Mar 2001
Externally publishedYes

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Viscosity measurement
Sedimentation
Hydrodynamics
Physical properties
Shear thinning
Guanidine
Polysaccharides
Velocity distribution
Molecular weight
Cyanobacteria
xanthan gum
Experiments

Cite this

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title = "Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: A comparison with xanthan",
abstract = "A bacterial exopolysaccharide from Aphanothece halophytica GR02 (AH-EPS) solubilized in buffered 1 M guanidine hydrochloride, GuHCl was studied using capillary viscometry, double gap viscometry, sedimentation velocity and sedimentation equilibrium using analytical ultracentrifugation. Results from capillary viscometry indicate that the exopolysaccharide is xanthan-like in its shear thinning properties. This was confirmed by double gap viscometry. Sedimentation velocity (distribution of apparent sedimentation coefficients) confirmed that the AH-EPS solution was reasonably homogeneous and a hyper-sharp boundary indicated that AH-EPS is a rigid/extra-rigid rod type polysaccharide. Sedimentation equilibrium confirmed the high molecular weight indicated by viscosity measurements. In order to ascertain whether the solution physical properties of AH-EPS are xanthan-like under the conditions studied, all the above experiments were repeated on a similarly treated xanthan solution in the same solvent. Results indicate that AH-EPS is indeed xanthan-like in its physical properties at least in the system studied. Xanthan properties in GuHCl do not appear too different from those studied in more traditional solvents.",
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Hydrodynamic characterization of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02 : A comparison with xanthan. / Morris, G. A.; Li, P.; Puaud, M.; Liu, Z.; Mitchell, J. R.; Harding, S. E.

In: Carbohydrate Polymers, Vol. 44, No. 3, 03.2001, p. 261-268.

Research output: Contribution to journalArticle

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