Identification and Localization of a New Human Myotubularin-Related Protein Gene, MTMR8, on 8p22–p23

Silke Appel, Kathrin Reichwald, Wolfgang Zimmermann, André Reis, André Rosenthal, Hans Christian Hennies

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Myotubularin and myotubularin-related proteins are dual-specificity phosphatases. Several myotubularin-related proteins have been identified in humans and mice. The members of the myotubularin protein family are highly conserved, from humans to yeast. Mutations in the human myotubularin gene (MTM1) lead to X-linked myotubular myopathy. Here we isolate and localize a novel putative myotubularin-related protein gene (MTMR8) on chromosome 8p22-p23, between the markers D8S550 and D8S265, by exon-trapping experiments and RT-PCR. Genomic sequencing revealed that the gene consists of 10 exons and spans approximately 43 kb. The corresponding cDNA is 7081 bp. The open reading frame predicts a protein of 549 amino acids and a calculated molecular mass of 63 kDa. Like myotubularin-related protein-5, MTMR8 has no dual-specificity phosphatase domain. It contains a double-helical motif similar to the SET interaction domain, which is thought to have a role in the control of cell proliferation.

LanguageEnglish
Pages6-8
Number of pages3
JournalGenomics
Volume75
Issue number1-3
DOIs
Publication statusPublished - Jul 2001
Externally publishedYes

Fingerprint

Dual-Specificity Phosphatases
Proteins
Exons
Congenital Structural Myopathies
myotubularin
Open Reading Frames
Genes
Complementary DNA
Chromosomes
Yeasts
Cell Proliferation
Amino Acids
Polymerase Chain Reaction
Mutation

Cite this

Appel, Silke ; Reichwald, Kathrin ; Zimmermann, Wolfgang ; Reis, André ; Rosenthal, André ; Hennies, Hans Christian. / Identification and Localization of a New Human Myotubularin-Related Protein Gene, MTMR8, on 8p22–p23. In: Genomics. 2001 ; Vol. 75, No. 1-3. pp. 6-8.
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Identification and Localization of a New Human Myotubularin-Related Protein Gene, MTMR8, on 8p22–p23. / Appel, Silke; Reichwald, Kathrin; Zimmermann, Wolfgang; Reis, André; Rosenthal, André; Hennies, Hans Christian.

In: Genomics, Vol. 75, No. 1-3, 07.2001, p. 6-8.

Research output: Contribution to journalArticle

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