Immune responses to V antigen of Yersinia pestis co-encapsulated with IFN-γ

Effect of dose and formulation

K. F. Griffin, B. R. Conway, H. O. Alpar, E. D. Williamson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Induction of systemic immune responses after intraperitoneal inoculation of poly(L)lactide microspheres containing the V antigen of Yersinia pestis co-encapsulated with IFN-γ were investigated. Serum antibody responses and T cell proliferative responses were measured in groups of Balb/c mice which were injected intraoperitoneally with single or double emulsion preparations of either V/IFN-γ or V alone in a range of dose levels. Groups which received V antigen co-encapsulated with IFN-γ produced higher V-specific antibody responses, predominantly of the IgG1 isotype. Administration of 25 μg V/IFN-γ in a single emulsion resulted in a significantly increased (p < 0.05) splenic T cell proliferative response to V antigen compared with other formulations. It was concluded that IFN-γ co-encapsulated with V antigen in poly(L)lactide microspheres acted as an adjuvant and increased antigen specific systemic immune responses. Therefore, co-encapsulation with IFN-γ may result in effective single dose vaccines by increasing the immunogenicity of the formulations.

Original languageEnglish
Pages (from-to)517-521
Number of pages5
JournalVaccine
Volume16
Issue number5
DOIs
Publication statusPublished - 1 Mar 1998
Externally publishedYes

Fingerprint

Yersinia pestis
immune response
antigens
Antigens
dosage
Emulsions
Microspheres
Antibody Formation
emulsions
T-lymphocytes
T-Lymphocytes
antibodies
encapsulation
adjuvants
Immunoglobulin G
vaccination
vaccines
mice
Serum

Cite this

Griffin, K. F. ; Conway, B. R. ; Alpar, H. O. ; Williamson, E. D. / Immune responses to V antigen of Yersinia pestis co-encapsulated with IFN-γ : Effect of dose and formulation. In: Vaccine. 1998 ; Vol. 16, No. 5. pp. 517-521.
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abstract = "Induction of systemic immune responses after intraperitoneal inoculation of poly(L)lactide microspheres containing the V antigen of Yersinia pestis co-encapsulated with IFN-γ were investigated. Serum antibody responses and T cell proliferative responses were measured in groups of Balb/c mice which were injected intraoperitoneally with single or double emulsion preparations of either V/IFN-γ or V alone in a range of dose levels. Groups which received V antigen co-encapsulated with IFN-γ produced higher V-specific antibody responses, predominantly of the IgG1 isotype. Administration of 25 μg V/IFN-γ in a single emulsion resulted in a significantly increased (p < 0.05) splenic T cell proliferative response to V antigen compared with other formulations. It was concluded that IFN-γ co-encapsulated with V antigen in poly(L)lactide microspheres acted as an adjuvant and increased antigen specific systemic immune responses. Therefore, co-encapsulation with IFN-γ may result in effective single dose vaccines by increasing the immunogenicity of the formulations.",
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Immune responses to V antigen of Yersinia pestis co-encapsulated with IFN-γ : Effect of dose and formulation. / Griffin, K. F.; Conway, B. R.; Alpar, H. O.; Williamson, E. D.

In: Vaccine, Vol. 16, No. 5, 01.03.1998, p. 517-521.

Research output: Contribution to journalArticle

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