Improvement of 1, 3-propanediol production with a recombinant strain Klebsiella pneumoniae F-1 by regulating redox potential

Zhi Hua Huang, Yan Ping Zhang, Chen Yu Du, Xing Huang, Zhu An Cao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Redox potential (also called oxidoreduction potential, ORP) is one of important indicators to monitor the anaerobic fermentation of Klebsiella pneumoniae. In this report, the preferred ORP of a recombinant strain K.pneumoniae F-1 was investigated by controlling the ORP of broth into four levels of -190, -210, -240 and -290 mV. The results showed that in the fermentation of ORP at -240 mV, the highest final 1, 3-propanediol concentration and molar yield of were achieved, being 81.5 g/L and 0.423 mol/mol, respectively. This preferred ORP to the genetic modified strain was different from those of both the wild strain K.pneumoniae M5aL (-190 mV) and the mutant K.pneumoniae M5aL YMU2 (-280 mV). It is demonstrated that the modification of the metabolic pathway in the K. pneumoniae F-1 exerted effects on the preferred ORP of the strain. The effect of ORP on re-distribution of the metabolites was also investigated. The results demonstrated that the reduction environment facilitated 'the pathways from glycerol to reduction end-metabolites, such as 1, 3-propanediol, ethanol and 2, 3-butanediol. This work is the first investigation on the ORP regulation method in a recombinant strain, which will provide insight into the mechanism of ORP regulation in anaerobic fermentations.

Original languageEnglish
Pages (from-to)1014-1017
Number of pages4
JournalGuocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering
Volume7
Issue number5
Publication statusPublished - 1 Oct 2007
Externally publishedYes

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Fermentation
Metabolites
Glycerol
Ethanol
1,3-propanediol
Oxidation-Reduction
Metabolic Networks and Pathways
2,3-butylene glycol

Cite this

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title = "Improvement of 1, 3-propanediol production with a recombinant strain Klebsiella pneumoniae F-1 by regulating redox potential",
abstract = "Redox potential (also called oxidoreduction potential, ORP) is one of important indicators to monitor the anaerobic fermentation of Klebsiella pneumoniae. In this report, the preferred ORP of a recombinant strain K.pneumoniae F-1 was investigated by controlling the ORP of broth into four levels of -190, -210, -240 and -290 mV. The results showed that in the fermentation of ORP at -240 mV, the highest final 1, 3-propanediol concentration and molar yield of were achieved, being 81.5 g/L and 0.423 mol/mol, respectively. This preferred ORP to the genetic modified strain was different from those of both the wild strain K.pneumoniae M5aL (-190 mV) and the mutant K.pneumoniae M5aL YMU2 (-280 mV). It is demonstrated that the modification of the metabolic pathway in the K. pneumoniae F-1 exerted effects on the preferred ORP of the strain. The effect of ORP on re-distribution of the metabolites was also investigated. The results demonstrated that the reduction environment facilitated 'the pathways from glycerol to reduction end-metabolites, such as 1, 3-propanediol, ethanol and 2, 3-butanediol. This work is the first investigation on the ORP regulation method in a recombinant strain, which will provide insight into the mechanism of ORP regulation in anaerobic fermentations.",
keywords = "1, 3-propanediol, Cofactor (NADH) regeneration, Klebsiella pneumoniae, Redox potential",
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Improvement of 1, 3-propanediol production with a recombinant strain Klebsiella pneumoniae F-1 by regulating redox potential. / Huang, Zhi Hua; Zhang, Yan Ping; Du, Chen Yu; Huang, Xing; Cao, Zhu An.

In: Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering, Vol. 7, No. 5, 01.10.2007, p. 1014-1017.

Research output: Contribution to journalArticle

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T1 - Improvement of 1, 3-propanediol production with a recombinant strain Klebsiella pneumoniae F-1 by regulating redox potential

AU - Huang, Zhi Hua

AU - Zhang, Yan Ping

AU - Du, Chen Yu

AU - Huang, Xing

AU - Cao, Zhu An

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AB - Redox potential (also called oxidoreduction potential, ORP) is one of important indicators to monitor the anaerobic fermentation of Klebsiella pneumoniae. In this report, the preferred ORP of a recombinant strain K.pneumoniae F-1 was investigated by controlling the ORP of broth into four levels of -190, -210, -240 and -290 mV. The results showed that in the fermentation of ORP at -240 mV, the highest final 1, 3-propanediol concentration and molar yield of were achieved, being 81.5 g/L and 0.423 mol/mol, respectively. This preferred ORP to the genetic modified strain was different from those of both the wild strain K.pneumoniae M5aL (-190 mV) and the mutant K.pneumoniae M5aL YMU2 (-280 mV). It is demonstrated that the modification of the metabolic pathway in the K. pneumoniae F-1 exerted effects on the preferred ORP of the strain. The effect of ORP on re-distribution of the metabolites was also investigated. The results demonstrated that the reduction environment facilitated 'the pathways from glycerol to reduction end-metabolites, such as 1, 3-propanediol, ethanol and 2, 3-butanediol. This work is the first investigation on the ORP regulation method in a recombinant strain, which will provide insight into the mechanism of ORP regulation in anaerobic fermentations.

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