In-Situ Biofilm Formation in Hyper Alkaline Environments

Christopher Charles, Simon Rout, Richard Wormald, Andrew Laws, B. R. Jackson, S. A. Boxall, Paul Humphreys

Research output: Contribution to journalArticle

Abstract

Lime manufacture in the UK has resulted in the generation of a number of alkaline sites (>pH 11.0) with complex indigenous microbial populations. Within the present study, retrievable cotton samples were used to investigate the fate of cellulose, the primary carbon source, within three sites aged from ≈25 to 140 years. Following 3 months incubation in situ, biofilms had formed on all cotton samples in these extreme pH conditions; with matrices comprised of carbohydrates, proteins, lipids and eDNA. Biofilms from the older sites contained greater amounts of eDNA, a structural component that aids the production of a denser biofilm. The age of the sites correlated with a shift from polysaccharides composed of β 1,4 and β 1,3 linked sugars to those composed of pyranosyl sugars within the older sites. These changes were reflected in the active biofilm communities which shifted from being Clostridiales dominated in the youngest site to Proteobacteria dominated in the older sites. The study demonstrates that the microbial communities resident in anthropogenic alkaline sites are able to form biofilms at pH values > pH 11.0 and that these biofilms evolve toward Proteobacteria dominated communities employing eDNA and pyranosyl sugar based polysaccharides to build the biofilm matrix.
Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalGeomicrobiology Journal
Volume36
Issue number5
Early online date28 Jan 2019
DOIs
Publication statusPublished - 28 May 2019

Fingerprint

alkaline environment
Biofilms
biofilm
Sugars
Proteobacteria
sugar
polysaccharide
Cotton
Polysaccharides
cotton
matrix
structural component
in situ
Population Groups
Cellulose
lime
cellulose
carbohydrate
microbial community
Carbon

Cite this

Charles, Christopher ; Rout, Simon ; Wormald, Richard ; Laws, Andrew ; Jackson, B. R. ; Boxall, S. A. ; Humphreys, Paul. / In-Situ Biofilm Formation in Hyper Alkaline Environments. In: Geomicrobiology Journal. 2019 ; Vol. 36, No. 5. pp. 405-411.
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In-Situ Biofilm Formation in Hyper Alkaline Environments. / Charles, Christopher; Rout, Simon; Wormald, Richard; Laws, Andrew; Jackson, B. R. ; Boxall, S. A. ; Humphreys, Paul.

In: Geomicrobiology Journal, Vol. 36, No. 5, 28.05.2019, p. 405-411.

Research output: Contribution to journalArticle

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