In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel: complementary electron microprobe and synchrotron microbeam X-ray analyses

A J Morgan, J F W Mosselmans, J M Charnock, A Bennett, C Winters, M O'Reilly, P Fisher, J Andre, M Turner, P Gunning, P Kille

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Understanding the relationships between accumulated metal speciation in cells and tissues of ecologically significant taxa such as earthworms will improve risk assessments. Synchrotron-based μ-focus X-ray spectroscopy was used to detect, localize, and determine ligand-speciation of Zn and Pb in thin sections of two epigeic earthworm species collected from a Pb/Zn-mine soil. The findings indicated that Zn and Pb partition predominantly as typical hard acids (i.e., strong affinities for O-donors) within liverlike chloragocytes. Moreover, Zn speciation was very similar in the chloragog and intestinal epithelia but differed subtly in the kidneylike nephridial tubules; neither Zn nor Pb was detectable in the ventral nerve cord. High resolution X-ray mapping of high pressure-frozen, ultrathin, freeze-substituted sections in a transmission electron microscope (TEM), combined with conventional TEM structural analysis, identified a new cell type packed with highly organized rough endoplasmic reticulum and containing deposits of Cd (codistributed with S); there was no evidence that these cells are major depositories of Zn or Pb. These data may be used in a systems biology approach to assist in the interpretation of metal-evoked perturbations in whole-worm transcriptome and metabolome profiles.

LanguageEnglish
Pages1073-1081
Number of pages9
JournalEnvironmental Science & Technology
Volume47
Issue number2
DOIs
Publication statusPublished - 15 Jan 2013
Externally publishedYes

Fingerprint

Synchrotrons
earthworm
electron probe analysis
ligand
Electron microscopes
Metals
Ligands
Soils
Imaging techniques
X rays
electron
Electrons
metal
structural analysis
X ray spectroscopy
thin section
Structural analysis
Risk assessment
X-ray spectroscopy
risk assessment

Cite this

Morgan, A J ; Mosselmans, J F W ; Charnock, J M ; Bennett, A ; Winters, C ; O'Reilly, M ; Fisher, P ; Andre, J ; Turner, M ; Gunning, P ; Kille, P. / In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel : complementary electron microprobe and synchrotron microbeam X-ray analyses. In: Environmental Science & Technology. 2013 ; Vol. 47, No. 2. pp. 1073-1081.
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Morgan, AJ, Mosselmans, JFW, Charnock, JM, Bennett, A, Winters, C, O'Reilly, M, Fisher, P, Andre, J, Turner, M, Gunning, P & Kille, P 2013, 'In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel: complementary electron microprobe and synchrotron microbeam X-ray analyses', Environmental Science & Technology, vol. 47, no. 2, pp. 1073-1081. https://doi.org/10.1021/es302633f

In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel : complementary electron microprobe and synchrotron microbeam X-ray analyses. / Morgan, A J; Mosselmans, J F W; Charnock, J M; Bennett, A; Winters, C; O'Reilly, M; Fisher, P; Andre, J; Turner, M; Gunning, P; Kille, P.

In: Environmental Science & Technology, Vol. 47, No. 2, 15.01.2013, p. 1073-1081.

Research output: Contribution to journalArticle

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T1 - In situ metal imaging and Zn ligand-speciation in a soil-dwelling sentinel

T2 - Environmental Science & Technology

AU - Morgan, A J

AU - Mosselmans, J F W

AU - Charnock, J M

AU - Bennett, A

AU - Winters, C

AU - O'Reilly, M

AU - Fisher, P

AU - Andre, J

AU - Turner, M

AU - Gunning, P

AU - Kille, P

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N2 - Understanding the relationships between accumulated metal speciation in cells and tissues of ecologically significant taxa such as earthworms will improve risk assessments. Synchrotron-based μ-focus X-ray spectroscopy was used to detect, localize, and determine ligand-speciation of Zn and Pb in thin sections of two epigeic earthworm species collected from a Pb/Zn-mine soil. The findings indicated that Zn and Pb partition predominantly as typical hard acids (i.e., strong affinities for O-donors) within liverlike chloragocytes. Moreover, Zn speciation was very similar in the chloragog and intestinal epithelia but differed subtly in the kidneylike nephridial tubules; neither Zn nor Pb was detectable in the ventral nerve cord. High resolution X-ray mapping of high pressure-frozen, ultrathin, freeze-substituted sections in a transmission electron microscope (TEM), combined with conventional TEM structural analysis, identified a new cell type packed with highly organized rough endoplasmic reticulum and containing deposits of Cd (codistributed with S); there was no evidence that these cells are major depositories of Zn or Pb. These data may be used in a systems biology approach to assist in the interpretation of metal-evoked perturbations in whole-worm transcriptome and metabolome profiles.

AB - Understanding the relationships between accumulated metal speciation in cells and tissues of ecologically significant taxa such as earthworms will improve risk assessments. Synchrotron-based μ-focus X-ray spectroscopy was used to detect, localize, and determine ligand-speciation of Zn and Pb in thin sections of two epigeic earthworm species collected from a Pb/Zn-mine soil. The findings indicated that Zn and Pb partition predominantly as typical hard acids (i.e., strong affinities for O-donors) within liverlike chloragocytes. Moreover, Zn speciation was very similar in the chloragog and intestinal epithelia but differed subtly in the kidneylike nephridial tubules; neither Zn nor Pb was detectable in the ventral nerve cord. High resolution X-ray mapping of high pressure-frozen, ultrathin, freeze-substituted sections in a transmission electron microscope (TEM), combined with conventional TEM structural analysis, identified a new cell type packed with highly organized rough endoplasmic reticulum and containing deposits of Cd (codistributed with S); there was no evidence that these cells are major depositories of Zn or Pb. These data may be used in a systems biology approach to assist in the interpretation of metal-evoked perturbations in whole-worm transcriptome and metabolome profiles.

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KW - Soil Pollutants/analysis

KW - Synchrotrons

KW - X-Ray Absorption Spectroscopy

KW - X-Rays

KW - Zinc/analysis

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