In vitro and in vivo responses of a panel of murine colon tumours to TCNU: a positive correlation

R. M. Phillips; M. C. Bibby; J. A. Double

doi:10.1016/0277-5379(88)90230-1

In vitro and in vivo responses of a panel of murine colon tumours to TCNU: a positive correlation

R. M. Phillips, M. C. Bibby, J. A. Double

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Abstract

TCNU is highly active against a panel of three histologically distinct transplantable murine adenocarcinomas of the colon (MAC tumours). Significant reductions in colony formation (>70%) were observed in vitro in all three cell lines following a 1 h exposure to TCNU at experimentally achievable drug C × t values. A good correlation exists between in vivo tumour responses and in vitro cell responses in all cases. Dose-response curves generated at increasing exposure times suggest that no active or long lived products of TCNU are formed as a result of the drug's spontaneous breakdown in vitro (rate of breakdown was 0.078 μg min^-1 at 37°C). Preliminary studies with the HT-29 human colon cell line have demonstrated that multi-cellular spheroids are more responsive to TCNU (2 ×) than the same cells cultured as monolayers.

Original language	English
Pages (from-to)	1365-1369,1371
Number of pages	6
Journal	European Journal of Cancer and Clinical Oncology
Volume	24
Issue number	8
DOIs	https://doi.org/10.1016/0277-5379(88)90230-1
Publication status	Published - Aug 1988
Externally published	Yes

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title = "In vitro and in vivo responses of a panel of murine colon tumours to TCNU: a positive correlation",

abstract = "TCNU is highly active against a panel of three histologically distinct transplantable murine adenocarcinomas of the colon (MAC tumours). Significant reductions in colony formation (>70%) were observed in vitro in all three cell lines following a 1 h exposure to TCNU at experimentally achievable drug C × t values. A good correlation exists between in vivo tumour responses and in vitro cell responses in all cases. Dose-response curves generated at increasing exposure times suggest that no active or long lived products of TCNU are formed as a result of the drug's spontaneous breakdown in vitro (rate of breakdown was 0.078 μg min-1 at 37°C). Preliminary studies with the HT-29 human colon cell line have demonstrated that multi-cellular spheroids are more responsive to TCNU (2 ×) than the same cells cultured as monolayers.",

keywords = "tauromustine, animal cell, colon cancer, Antineoplastic Agents, Cell Division, Cell Line, Colonic Neoplasms, Colony-Forming Units Assay, Dose-Response Relationship, Drug, Neoplasm Transplantation, Nitrosourea Compounds, Taurine, Tumor Cells, Cultured",

author = "Phillips, {R. M.} and Bibby, {M. C.} and Double, {J. A.}",

year = "1988",

month = aug,

doi = "10.1016/0277-5379(88)90230-1",

language = "English",

volume = "24",

pages = "1365--1369,1371",

journal = "European Journal of Cancer and Clinical Oncology",

issn = "0277-5379",

publisher = "Pergamon Press",

number = "8",

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TY - JOUR

T1 - In vitro and in vivo responses of a panel of murine colon tumours to TCNU

T2 - a positive correlation

AU - Phillips, R. M.

AU - Bibby, M. C.

AU - Double, J. A.

PY - 1988/8

Y1 - 1988/8

N2 - TCNU is highly active against a panel of three histologically distinct transplantable murine adenocarcinomas of the colon (MAC tumours). Significant reductions in colony formation (>70%) were observed in vitro in all three cell lines following a 1 h exposure to TCNU at experimentally achievable drug C × t values. A good correlation exists between in vivo tumour responses and in vitro cell responses in all cases. Dose-response curves generated at increasing exposure times suggest that no active or long lived products of TCNU are formed as a result of the drug's spontaneous breakdown in vitro (rate of breakdown was 0.078 μg min-1 at 37°C). Preliminary studies with the HT-29 human colon cell line have demonstrated that multi-cellular spheroids are more responsive to TCNU (2 ×) than the same cells cultured as monolayers.

AB - TCNU is highly active against a panel of three histologically distinct transplantable murine adenocarcinomas of the colon (MAC tumours). Significant reductions in colony formation (>70%) were observed in vitro in all three cell lines following a 1 h exposure to TCNU at experimentally achievable drug C × t values. A good correlation exists between in vivo tumour responses and in vitro cell responses in all cases. Dose-response curves generated at increasing exposure times suggest that no active or long lived products of TCNU are formed as a result of the drug's spontaneous breakdown in vitro (rate of breakdown was 0.078 μg min-1 at 37°C). Preliminary studies with the HT-29 human colon cell line have demonstrated that multi-cellular spheroids are more responsive to TCNU (2 ×) than the same cells cultured as monolayers.

KW - tauromustine

KW - animal cell

KW - colon cancer

KW - Antineoplastic Agents

KW - Cell Division

KW - Cell Line

KW - Colonic Neoplasms

KW - Colony-Forming Units Assay

KW - Dose-Response Relationship, Drug

KW - Neoplasm Transplantation

KW - Nitrosourea Compounds

KW - Taurine

KW - Tumor Cells, Cultured

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U2 - 10.1016/0277-5379(88)90230-1

DO - 10.1016/0277-5379(88)90230-1

M3 - Article

C2 - 3181258

AN - SCOPUS:0023761001

VL - 24

SP - 1365-1369,1371

JO - European Journal of Cancer and Clinical Oncology

JF - European Journal of Cancer and Clinical Oncology

SN - 0277-5379

IS - 8

ER -

In vitro and in vivo responses of a panel of murine colon tumours to TCNU: a positive correlation

Abstract

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