In vitro and in vivo responses of a panel of murine colon tumours to TCNU: a positive correlation

R. M. Phillips, M. C. Bibby, J. A. Double

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

TCNU is highly active against a panel of three histologically distinct transplantable murine adenocarcinomas of the colon (MAC tumours). Significant reductions in colony formation (>70%) were observed in vitro in all three cell lines following a 1 h exposure to TCNU at experimentally achievable drug C × t values. A good correlation exists between in vivo tumour responses and in vitro cell responses in all cases. Dose-response curves generated at increasing exposure times suggest that no active or long lived products of TCNU are formed as a result of the drug's spontaneous breakdown in vitro (rate of breakdown was 0.078 μg min-1 at 37°C). Preliminary studies with the HT-29 human colon cell line have demonstrated that multi-cellular spheroids are more responsive to TCNU (2 ×) than the same cells cultured as monolayers.

Original languageEnglish
Pages (from-to)1365-1369,1371
Number of pages6
JournalEuropean Journal of Cancer and Clinical Oncology
Volume24
Issue number8
DOIs
Publication statusPublished - Aug 1988
Externally publishedYes

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