In Vivo Optical Performance of a New Class of Near-Infrared-Emitting Conjugated Polymers: Borylated PF8-BT

Paul Robert Neumann, Daniel L. Crossley, Michael Turner, Michael Ingleson, Mark Green, Jianghong Rao, Lea Ann Dailey

Research output: Contribution to journalArticle

Abstract

Borylated poly(fluorene-benzothiadiazoles) (PF8-BT) are π-conjugated polymers (CPs) with deep-red/ near-infrared (NIR) absorption and emission profiles suitable for in vivo optical imaging. A fully borylated PF8-BT derivative (P4) was encapsulated in pegylated poly(lactic- co-glycolic acid) (PEG-PLGA) nanoparticles and compared with a reference NIR-emitting CP (PCPDTBT) or indocyanine green (ICG). All formulations satisfied quality requirements for parenterally administered diagnostics. P4 nanoparticles had higher quantum yield (2.3%) than PCPCDTBT (0.01%) or ICG nanoparticles (1.1%). The signal/background ratios (SBRs) of CP systems P4 and PCPDTBT in a phantom mouse (λ em = 820 nm) increased linearly with fluorophore mass (12.5-100 μg/mL), while the SBRs of ICG decreased above 25 μg/mL. P4 nanoparticles experienced <10% photobleaching over 10 irradiations (PCPDTBT: ∼25% and ICG: >44%). In a mouse tumor xenograft model, P4 nanoparticles showed a 5-fold higher SBR than PCPDTBT particles with fluorophore accumulation in the liver > spleen > tumor. Blood chemistry and tissue histology showed no abnormalities compared to untreated animals after a single administration.

Original languageEnglish
Pages (from-to)46525-46535
Number of pages11
JournalACS Applied Materials and Interfaces
Volume11
Issue number50
Early online date20 Nov 2019
DOIs
Publication statusPublished - 18 Dec 2019

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Conjugated polymers
Indocyanine Green
Nanoparticles
Infrared radiation
Fluorophores
Tumors
Histology
Infrared absorption
Quantum yield
Heterografts
Liver
Animals
Blood
Tissue
Derivatives
Imaging techniques
Acids
poly(2,6-(4,4-bis(2-ethylhexyl)-4H-cyclopenta(2,1-b-3,4-b0)dithiophene))-alt-4,7-(2,1,3-benzothiadiazole)

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Neumann, Paul Robert ; Crossley, Daniel L. ; Turner, Michael ; Ingleson, Michael ; Green, Mark ; Rao, Jianghong ; Dailey, Lea Ann. / In Vivo Optical Performance of a New Class of Near-Infrared-Emitting Conjugated Polymers : Borylated PF8-BT. In: ACS Applied Materials and Interfaces. 2019 ; Vol. 11, No. 50. pp. 46525-46535.
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In Vivo Optical Performance of a New Class of Near-Infrared-Emitting Conjugated Polymers : Borylated PF8-BT. / Neumann, Paul Robert; Crossley, Daniel L.; Turner, Michael; Ingleson, Michael; Green, Mark; Rao, Jianghong; Dailey, Lea Ann.

In: ACS Applied Materials and Interfaces, Vol. 11, No. 50, 18.12.2019, p. 46525-46535.

Research output: Contribution to journalArticle

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T1 - In Vivo Optical Performance of a New Class of Near-Infrared-Emitting Conjugated Polymers

T2 - Borylated PF8-BT

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AU - Crossley, Daniel L.

AU - Turner, Michael

AU - Ingleson, Michael

AU - Green, Mark

AU - Rao, Jianghong

AU - Dailey, Lea Ann

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