Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders

Katja Martina Eckl, Günther Weindl, Katharina Ackermann, Sarah Küchler, Ramona Casper, Michal R. Radowski, Rainer Haag, Hans Christian Hennies, Monika Schäfer-Korting

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13 Citations (Scopus)

Abstract

The aim of this study was to assess a recently established 3D model of congenital ichthyosis, representing severe epidermal barrier function defects, for skin penetration and permeation. We have generated disease models by knock-down of either TGM1 or ALOXE3 in primary human keratinocytes, and using keratinocytes and fibroblasts from patients with congenital ichthyosis. The results indicate disturbed barrier function as demonstrated by increased permeation of testosterone and caffeine particularly in TGM1 knock-down models compared to control models. In addition, enhanced penetration of the model dye nile red incorporated into solid lipid nanoparticles and core-multishell nanotransporters, respectively, was evident in disease models. Thus, in vitro skin disease models reproduce differences in barrier permeability and function seen in congenital ichthyosis and pave the way to personalised disease models. Furthermore, our findings indicate that nanocarriers may be useful in new, topical therapeutic approaches for the currently very limited treatment of congenital ichthyosis.

Original languageEnglish
Pages (from-to)286-288
Number of pages3
JournalExperimental Dermatology
Volume23
Issue number4
Early online date28 Mar 2014
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

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    Eckl, K. M., Weindl, G., Ackermann, K., Küchler, S., Casper, R., Radowski, M. R., Haag, R., Hennies, H. C., & Schäfer-Korting, M. (2014). Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders. Experimental Dermatology, 23(4), 286-288. https://doi.org/10.1111/exd.12366