Interaction of anesthetic supplement thiopental with human serum albumin

Shahper N. Khan, Barira Islam, M. R. Rajeswari, Hammad Usmani, Asad U. Khan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Thiopental (TPL) is a commonly used barbiturate anesthetic. Its binding with human serum albumin (HSA) was studied to explore the anesthetic-induced protein dysfunction. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. An increase in the binding affinity (K) and in the number of binding sites (n) with the increasing albumin concentration was observed. The interaction was conformation-dependent and the highest for the F isomer of HSA, which implicates its slow elimination. The mode of binding was characterized using various thermodynamic parameters. Domain II of HSA was found to possess a high affinity binding site for TPL. The effect of micro-metal ions on the binding affinity was also investigated. The molecular distance, r, between donor (HSA) and acceptor (TPL) was estimated by fluorescence resonance energy transfer (FRET). Correlation between the stability of the TPL-N and TPL-F complexes and drug distribution is discussed. The structural changes in the protein investigated by circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy reflect perturbation of the albumin molecule and provide an explanation for the heterogeneity of action of this anesthetic.

Original languageEnglish
Pages (from-to)399-409
Number of pages11
JournalActa Biochimica Polonica
Volume55
Issue number2
Publication statusPublished - 14 Jul 2008
Externally publishedYes

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Thiopental
Serum Albumin
Anesthetics
Albumins
Binding Sites
Fluorescence Resonance Energy Transfer
Fluorescence Spectrometry
Fluorescence spectroscopy
Dichroism
Fourier Transform Infrared Spectroscopy
Circular Dichroism
Ultraviolet spectroscopy
Absorption spectroscopy
Thermodynamics
Isomers
Metal ions
Conformations
Proteins
Metals
Ions

Cite this

Khan, S. N., Islam, B., Rajeswari, M. R., Usmani, H., & Khan, A. U. (2008). Interaction of anesthetic supplement thiopental with human serum albumin. Acta Biochimica Polonica, 55(2), 399-409.
Khan, Shahper N. ; Islam, Barira ; Rajeswari, M. R. ; Usmani, Hammad ; Khan, Asad U. / Interaction of anesthetic supplement thiopental with human serum albumin. In: Acta Biochimica Polonica. 2008 ; Vol. 55, No. 2. pp. 399-409.
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Khan, SN, Islam, B, Rajeswari, MR, Usmani, H & Khan, AU 2008, 'Interaction of anesthetic supplement thiopental with human serum albumin', Acta Biochimica Polonica, vol. 55, no. 2, pp. 399-409.

Interaction of anesthetic supplement thiopental with human serum albumin. / Khan, Shahper N.; Islam, Barira; Rajeswari, M. R.; Usmani, Hammad; Khan, Asad U.

In: Acta Biochimica Polonica, Vol. 55, No. 2, 14.07.2008, p. 399-409.

Research output: Contribution to journalArticle

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AB - Thiopental (TPL) is a commonly used barbiturate anesthetic. Its binding with human serum albumin (HSA) was studied to explore the anesthetic-induced protein dysfunction. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. An increase in the binding affinity (K) and in the number of binding sites (n) with the increasing albumin concentration was observed. The interaction was conformation-dependent and the highest for the F isomer of HSA, which implicates its slow elimination. The mode of binding was characterized using various thermodynamic parameters. Domain II of HSA was found to possess a high affinity binding site for TPL. The effect of micro-metal ions on the binding affinity was also investigated. The molecular distance, r, between donor (HSA) and acceptor (TPL) was estimated by fluorescence resonance energy transfer (FRET). Correlation between the stability of the TPL-N and TPL-F complexes and drug distribution is discussed. The structural changes in the protein investigated by circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy reflect perturbation of the albumin molecule and provide an explanation for the heterogeneity of action of this anesthetic.

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Khan SN, Islam B, Rajeswari MR, Usmani H, Khan AU. Interaction of anesthetic supplement thiopental with human serum albumin. Acta Biochimica Polonica. 2008 Jul 14;55(2):399-409.