Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei

Michael L. Ginger, E. Solange Ngazoa, Claudio A. Pereira, Timothy J. Pullen, Mostafa Kabiri, Katja Becker, Keith Gull, Dietmar Steverding

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Adenylate kinases occur classically as cytoplasmic and mitochondrial enzymes, but the expression of seven adenylate kinases in the flagellated protozoan parasite Trypanosoma brucei (order, Kinetoplastida; family, Trypanosomatidae) easily exceeds the number of isoforms previously observed within a single cell and raises questions as to their location and function. We show that a requirement to target adenylate kinase into glycosomes, which are unique kinetoplastid-specific microbodies of the peroxisome class in which many reactions of carbohydrate metabolism are compartmentalized, and two different flagellar structures as well as cytoplasm and mitochondrion explains the expansion of this gene family in trypanosomes. The three isoforms that are selectively built into either the flagellar axoneme or the extra-axonemal paraflagellar rod, which is essential for motility, all contain long N-terminal extensions. Biochemical analysis of the only short form trypanosome adenylate kinase revealed that this enzyme catalyzes phosphotransfer of γ-phosphate from ATP to AMP, CMP, and UMP acceptors; its high activity and specificity toward CMP is likely to reflect an adaptation to very low intracellular cytidine nucleotide pools. Analysis of some of the phosphotransfer network using RNA interference suggests considerable complexity within the homeostasis of cellular energetics. The anchoring of specific adenylate kinases within two distinct flagellar structures provides a paradigm for metabolic organization and efficiency in other flagellates.

Original languageEnglish
Pages (from-to)11781-11789
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number12
Early online date18 Jan 2005
DOIs
Publication statusPublished - 25 Mar 2005
Externally publishedYes

Fingerprint

Adenylate Kinase
Trypanosoma brucei brucei
Protein Isoforms
Parasites
Genes
Microbodies
Cytidine Monophosphate
Trypanosomiasis
Kinetoplastida
Trypanosomatina
Uridine Monophosphate
Axoneme
Cytidine
Mitochondria
Peroxisomes
Carbohydrate Metabolism
Enzymes
Adenosine Monophosphate
RNA Interference
Cytoplasm

Cite this

Ginger, Michael L. ; Ngazoa, E. Solange ; Pereira, Claudio A. ; Pullen, Timothy J. ; Kabiri, Mostafa ; Becker, Katja ; Gull, Keith ; Steverding, Dietmar. / Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 12. pp. 11781-11789.
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Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei. / Ginger, Michael L.; Ngazoa, E. Solange; Pereira, Claudio A.; Pullen, Timothy J.; Kabiri, Mostafa; Becker, Katja; Gull, Keith; Steverding, Dietmar.

In: Journal of Biological Chemistry, Vol. 280, No. 12, 25.03.2005, p. 11781-11789.

Research output: Contribution to journalArticle

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