Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol

Yanping Zhang, Zhihua Huang, Chenyu Du, Yin Li, Zhu'an Cao

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Redox cofactors play crucial roles in the metabolic and regulatory network of living organisms. We reported here the effect of introducing a heterogeneous NADH regeneration system into Klebsiella oxytoca on cell growth and glycerol metabolism. Expression of fdh gene from Candida boidinii in K. oxytoca resulted in higher intracellular concentrations of both NADH and NAD+ during the fermentation metaphase, with the ratio of NADH to NAD+ unaltered and cell growth unaffected, interestingly different from that in engineered Escherichia coli, Lactococcus lactis, and others. Metabolic flux analysis revealed that fluxes to 1,3-propanediol, ethanol, and lactate were all increased, suggesting both the oxidative and reductive metabolisms of glycerol were enhanced. It demonstrates that in certain microbial system NADH availability can be increased with NADH to NAD+ ratio unaltered, providing a new strategy to improve the metabolic flux in those microorganisms where glycolysis is not the only central metabolic pathways.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalMetabolic Engineering
Volume11
Issue number2
DOIs
Publication statusPublished - 1 Mar 2009
Externally publishedYes

Fingerprint

Klebsiella oxytoca
Glycerol
Metabolism
NAD
Regeneration
Cell growth
Fluxes
Candida
Microorganisms
Fermentation
Escherichia coli
Ethanol
Genes
Metabolic Networks and Pathways
Availability
Metabolic Flux Analysis
Lactococcus lactis
Glycolysis
Metaphase
Growth

Cite this

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abstract = "Redox cofactors play crucial roles in the metabolic and regulatory network of living organisms. We reported here the effect of introducing a heterogeneous NADH regeneration system into Klebsiella oxytoca on cell growth and glycerol metabolism. Expression of fdh gene from Candida boidinii in K. oxytoca resulted in higher intracellular concentrations of both NADH and NAD+ during the fermentation metaphase, with the ratio of NADH to NAD+ unaltered and cell growth unaffected, interestingly different from that in engineered Escherichia coli, Lactococcus lactis, and others. Metabolic flux analysis revealed that fluxes to 1,3-propanediol, ethanol, and lactate were all increased, suggesting both the oxidative and reductive metabolisms of glycerol were enhanced. It demonstrates that in certain microbial system NADH availability can be increased with NADH to NAD+ ratio unaltered, providing a new strategy to improve the metabolic flux in those microorganisms where glycolysis is not the only central metabolic pathways.",
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Introduction of an NADH regeneration system into Klebsiella oxytoca leads to an enhanced oxidative and reductive metabolism of glycerol. / Zhang, Yanping; Huang, Zhihua; Du, Chenyu; Li, Yin; Cao, Zhu'an.

In: Metabolic Engineering, Vol. 11, No. 2, 01.03.2009, p. 101-106.

Research output: Contribution to journalArticle

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