Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface

Muhammad Mahdi Abdul Jamil, Mohamed Ahmed Milad Zaltum, Nur Adilah Abd Rahman, Razdi Ambar, Morgan Denyer, Farideh Javid, Farshid Sefat, Masoud Mozafari

Research output: Contribution to journalConference article

Abstract

Cell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM) molecules including collagen, fibronectin and laminin can be examined using micro contact printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration, differentiation and network formation in-vitro. This study investigates the effect of microcontact printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of
25µm, 50µm and 100µm width. However, HeLa cells seeded on 50µm induced the best alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50µm wide fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was influenced by 100µs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachment
and cell guidance. Understanding this further may have applications in enhancing tissue graft generation and potentially wound repair.
Original languageEnglish
Article number012018
Number of pages10
JournalJournal of Physics
Volume1019
Issue number1
Early online date27 Jun 2018
DOIs
Publication statusPublished - 12 Jul 2018
Event1st International Conference on Green and Sustainable Computing - Hilton Kuching, Sarawak, Malaysia
Duration: 25 Nov 201727 Nov 2017
https://icoges2017.weebly.com/

Fingerprint

alignment
proteins
electric fields
matrices
pulses
cells
adhesion
collagens
plains
cultured cells
printing
slip
glass
molecules

Cite this

Jamil, Muhammad Mahdi Abdul ; Zaltum, Mohamed Ahmed Milad ; Rahman, Nur Adilah Abd ; Ambar, Razdi ; Denyer, Morgan ; Javid, Farideh ; Sefat, Farshid ; Mozafari, Masoud. / Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface. In: Journal of Physics. 2018 ; Vol. 1019, No. 1.
@article{fbb7c4f2adfb4c2b8fdd207ed7bd6f35,
title = "Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface",
abstract = "Cell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM) molecules including collagen, fibronectin and laminin can be examined using micro contact printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration, differentiation and network formation in-vitro. This study investigates the effect of microcontact printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of25µm, 50µm and 100µm width. However, HeLa cells seeded on 50µm induced the best alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50µm wide fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was influenced by 100µs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachmentand cell guidance. Understanding this further may have applications in enhancing tissue graft generation and potentially wound repair.",
keywords = "cell guidance, cell proliferation, cells alignment, extra cellular matrix, HeLa, micro contact printing, pulse electric field",
author = "Jamil, {Muhammad Mahdi Abdul} and Zaltum, {Mohamed Ahmed Milad} and Rahman, {Nur Adilah Abd} and Razdi Ambar and Morgan Denyer and Farideh Javid and Farshid Sefat and Masoud Mozafari",
year = "2018",
month = "7",
day = "12",
doi = "10.1088/1742-6596/1019/1/012018",
language = "English",
volume = "1019",
journal = "Journal of Physics",
number = "1",

}

Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface. / Jamil, Muhammad Mahdi Abdul; Zaltum, Mohamed Ahmed Milad; Rahman, Nur Adilah Abd; Ambar, Razdi; Denyer, Morgan; Javid, Farideh; Sefat, Farshid; Mozafari, Masoud.

In: Journal of Physics, Vol. 1019, No. 1, 012018, 12.07.2018.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface

AU - Jamil, Muhammad Mahdi Abdul

AU - Zaltum, Mohamed Ahmed Milad

AU - Rahman, Nur Adilah Abd

AU - Ambar, Razdi

AU - Denyer, Morgan

AU - Javid, Farideh

AU - Sefat, Farshid

AU - Mozafari, Masoud

PY - 2018/7/12

Y1 - 2018/7/12

N2 - Cell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM) molecules including collagen, fibronectin and laminin can be examined using micro contact printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration, differentiation and network formation in-vitro. This study investigates the effect of microcontact printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of25µm, 50µm and 100µm width. However, HeLa cells seeded on 50µm induced the best alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50µm wide fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was influenced by 100µs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachmentand cell guidance. Understanding this further may have applications in enhancing tissue graft generation and potentially wound repair.

AB - Cell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM) molecules including collagen, fibronectin and laminin can be examined using micro contact printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration, differentiation and network formation in-vitro. This study investigates the effect of microcontact printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of25µm, 50µm and 100µm width. However, HeLa cells seeded on 50µm induced the best alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50µm wide fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was influenced by 100µs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachmentand cell guidance. Understanding this further may have applications in enhancing tissue graft generation and potentially wound repair.

KW - cell guidance

KW - cell proliferation

KW - cells alignment

KW - extra cellular matrix

KW - HeLa

KW - micro contact printing

KW - pulse electric field

UR - http://www.scopus.com/inward/record.url?scp=85049905202&partnerID=8YFLogxK

U2 - 10.1088/1742-6596/1019/1/012018

DO - 10.1088/1742-6596/1019/1/012018

M3 - Conference article

VL - 1019

JO - Journal of Physics

JF - Journal of Physics

IS - 1

M1 - 012018

ER -