Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver

Douglas J. Clarke; Jeffrey N. Keen; Brian Burchell

doi:10.1016/0014-5793(92)80243-A

Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver

Douglas J. Clarke, Jeffrey N. Keen, Brian Burchell

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Abstract

A novel UDP-glucuronosyltransferase that conjugates bilirubin IXα, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.

Original language	English
Pages (from-to)	183-186
Number of pages	4
Journal	FEBS Letters
Volume	299
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)80243-A
Publication status	Published - 9 Mar 1992
Externally published	Yes

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title = "Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver",

abstract = "A novel UDP-glucuronosyltransferase that conjugates bilirubin IXα, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.",

keywords = "Acylglucuronide, Arylalkanoic acid, Congenital jaundice, Glycosylation, UGT1 subfamily",

author = "Clarke, {Douglas J.} and Keen, {Jeffrey N.} and Brian Burchell",

year = "1992",

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T1 - Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver

AU - Clarke, Douglas J.

AU - Keen, Jeffrey N.

AU - Burchell, Brian

PY - 1992/3/9

Y1 - 1992/3/9

N2 - A novel UDP-glucuronosyltransferase that conjugates bilirubin IXα, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.

AB - A novel UDP-glucuronosyltransferase that conjugates bilirubin IXα, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.

KW - Acylglucuronide

KW - Arylalkanoic acid

KW - Congenital jaundice

KW - Glycosylation

KW - UGT1 subfamily

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EP - 186

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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ER -

Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver

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