Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver

Douglas J. Clarke, Jeffrey N. Keen, Brian Burchell

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A novel UDP-glucuronosyltransferase that conjugates bilirubin IXα, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.

LanguageEnglish
Pages183-186
Number of pages4
JournalFEBS Letters
Volume299
Issue number2
DOIs
Publication statusPublished - 9 Mar 1992
Externally publishedYes

Fingerprint

bilirubin glucuronoside glucuronosyltransferase
Gunn Rats
Liver
Rats
Enzymes
Clofibrate
Glucuronosyltransferase
Protein Sequence Analysis
Molecular mass
Bilirubin
Wistar Rats
Peptides
Acids

Cite this

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abstract = "A novel UDP-glucuronosyltransferase that conjugates bilirubin IXα, bilirubin monoglucuronide and an arylalkanoic acid was purified to homogeneity from clofibrate treated Wistar rats. The enzyme displayed a subunit molecular mass of 54 kDa, a pI of 7.6 and was demonstrated to be N-glycosylated. Sequence analysis of peptides derived by endoproteinase Glu-C cleavage of the purified enzyme indicated that it was a new member of the recently identified UGT1 subfamily. Immunoblot analysis demonstrated that this enzyme was absent from Gunn rat liver. The molecular derivation of this enzyme and the lack of it in Gunn rats is discussed.",
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Isolation and characterisation of a new hepatic bilirubin UDP-glucuronosyltransferase Absence from Gunn rat liver. / Clarke, Douglas J.; Keen, Jeffrey N.; Burchell, Brian.

In: FEBS Letters, Vol. 299, No. 2, 09.03.1992, p. 183-186.

Research output: Contribution to journalArticle

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