Isolation and characterization of a high molecular mass β-glucan from Lactobacillus fermentum Lf2 and evaluation of its immunomodulatory activity

Ana Vitlic, Sohaib Sadiq, Hafiz Ahmed, Elisa Ale, Ana Binetti, Andrew Collett, Paul Humphreys, Andrew Laws

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2 Citations (Scopus)

Abstract

When grown in a semi-defined medium, L. fermentum Lf2 synthesizes significant quantities (∼2 g/L) of two exopolysaccharides (EPS). The two EPS were separated by preparative size exclusion chromatography to give a high molecular mass β-glucan (1.23 × 10 6 Da) and a medium molecular mass heteroglycan (8.8 × 10 4 Da). The structure of the high molecular mass β-glucan was determined using a combination of NMR spectroscopy, monomer and linkage analysis. The EPS has the following structure:(Figure presented.) The immunomodulatory activity of the high molecular mass EPS was studied in peripheral blood mononuclear cells (PBMC). Exposure of PBMC to an aqueous solution of the EPS for 24 h led to increased cell proliferation, changes in expression of the cytokines CD14 and TLR2, and to an increase in production of TNF-α compared to controls. In contrast, when cells that had been treated with EPS for 24 h and from which the EPS had been removed, were subsequently exposed to the bacterial antigen LPS very low levels of TNF-α production were observed. This result indicates that the EPS imparts immunotolerance in PBMC. An ability to modulate the release of the proinflammatory mediators, such as TNF-α, is an important goal in the development of therapies for the treatment of diseases, such as Crohn's disease and ulcerative colitis, associated with excessive release of inflammatory mediators.

LanguageEnglish
Pages44-52
Number of pages9
JournalCarbohydrate Research
Volume476
Early online date9 Mar 2019
DOIs
Publication statusPublished - 1 Apr 2019

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Lactobacillus fermentum
beta-Glucans
Molecular mass
Blood Cells
Tumor Necrosis Factor-alpha
Blood
Bacterial Antigens
Mass Media
Molecular Structure
Ulcerative Colitis
Crohn Disease
Gel Chromatography
Size exclusion chromatography
Cell proliferation
Magnetic Resonance Spectroscopy
Cell Proliferation
Cytokines
Nuclear magnetic resonance spectroscopy
Monomers
Therapeutics

Cite this

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title = "Isolation and characterization of a high molecular mass β-glucan from Lactobacillus fermentum Lf2 and evaluation of its immunomodulatory activity",
abstract = "When grown in a semi-defined medium, L. fermentum Lf2 synthesizes significant quantities (∼2 g/L) of two exopolysaccharides (EPS). The two EPS were separated by preparative size exclusion chromatography to give a high molecular mass β-glucan (1.23 × 10 6 Da) and a medium molecular mass heteroglycan (8.8 × 10 4 Da). The structure of the high molecular mass β-glucan was determined using a combination of NMR spectroscopy, monomer and linkage analysis. The EPS has the following structure:(Figure presented.) The immunomodulatory activity of the high molecular mass EPS was studied in peripheral blood mononuclear cells (PBMC). Exposure of PBMC to an aqueous solution of the EPS for 24 h led to increased cell proliferation, changes in expression of the cytokines CD14 and TLR2, and to an increase in production of TNF-α compared to controls. In contrast, when cells that had been treated with EPS for 24 h and from which the EPS had been removed, were subsequently exposed to the bacterial antigen LPS very low levels of TNF-α production were observed. This result indicates that the EPS imparts immunotolerance in PBMC. An ability to modulate the release of the proinflammatory mediators, such as TNF-α, is an important goal in the development of therapies for the treatment of diseases, such as Crohn's disease and ulcerative colitis, associated with excessive release of inflammatory mediators.",
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T1 - Isolation and characterization of a high molecular mass β-glucan from Lactobacillus fermentum Lf2 and evaluation of its immunomodulatory activity

AU - Vitlic, Ana

AU - Sadiq, Sohaib

AU - Ahmed, Hafiz

AU - Ale, Elisa

AU - Binetti, Ana

AU - Collett, Andrew

AU - Humphreys, Paul

AU - Laws, Andrew

PY - 2019/4/1

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AB - When grown in a semi-defined medium, L. fermentum Lf2 synthesizes significant quantities (∼2 g/L) of two exopolysaccharides (EPS). The two EPS were separated by preparative size exclusion chromatography to give a high molecular mass β-glucan (1.23 × 10 6 Da) and a medium molecular mass heteroglycan (8.8 × 10 4 Da). The structure of the high molecular mass β-glucan was determined using a combination of NMR spectroscopy, monomer and linkage analysis. The EPS has the following structure:(Figure presented.) The immunomodulatory activity of the high molecular mass EPS was studied in peripheral blood mononuclear cells (PBMC). Exposure of PBMC to an aqueous solution of the EPS for 24 h led to increased cell proliferation, changes in expression of the cytokines CD14 and TLR2, and to an increase in production of TNF-α compared to controls. In contrast, when cells that had been treated with EPS for 24 h and from which the EPS had been removed, were subsequently exposed to the bacterial antigen LPS very low levels of TNF-α production were observed. This result indicates that the EPS imparts immunotolerance in PBMC. An ability to modulate the release of the proinflammatory mediators, such as TNF-α, is an important goal in the development of therapies for the treatment of diseases, such as Crohn's disease and ulcerative colitis, associated with excessive release of inflammatory mediators.

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