Isolation and characterization of a novel exopolysaccharide secreted by Lactobacillus mucosae VG1.

Omololu Fagunwa, Hafiz Ahmed, Sohaib Sadiq, Paul Humphreys, Neil Mclay, Andrew Laws

Research output: Contribution to journalArticle

Abstract

A novel strain of Lactobacillus mucosae was isolated from a faecal sample of an individual who had adhered to a strict vegetarian diet for nine years. The strain displayed a ropy character when grown on plates and generated a relatively small amount (62 mg/L) of an exopolysaccharide (EPS) when grown in broth culture. The EPS eluted from a size exclusion chromatography column as a single band with a weight average molecular mass of 1.51 × 10 4 g/mol. Monomer analysis and sugar absolute configuration analysis confirmed that the EPS was a D-galactan. Using linkage analysis in combination with 1D and 2D-NMR spectroscopy, with spectra being recorded for both the native EPS and for the products generated by Smith degradation of the EPS, the following structure was determined for the repeat unit of the polysaccharide:[Figure presented] This is a novel D-galactan and represents the first structure for an EPS produced by a strain of Lactobacillus mucosae to be reported.

Original languageEnglish
Article number107781
Number of pages8
JournalCarbohydrate Research
Volume484
Early online date22 Aug 2019
DOIs
Publication statusPublished - 1 Oct 2019

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Lactobacillus
Mucous Membrane
Vegetarian Diet
Size exclusion chromatography
Molecular mass
Nutrition
Sugars
Nuclear magnetic resonance spectroscopy
Gel Chromatography
Polysaccharides
Magnetic Resonance Spectroscopy
Monomers
Molecular Weight
Degradation
galactan F

Cite this

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abstract = "A novel strain of Lactobacillus mucosae was isolated from a faecal sample of an individual who had adhered to a strict vegetarian diet for nine years. The strain displayed a ropy character when grown on plates and generated a relatively small amount (62 mg/L) of an exopolysaccharide (EPS) when grown in broth culture. The EPS eluted from a size exclusion chromatography column as a single band with a weight average molecular mass of 1.51 × 10 4 g/mol. Monomer analysis and sugar absolute configuration analysis confirmed that the EPS was a D-galactan. Using linkage analysis in combination with 1D and 2D-NMR spectroscopy, with spectra being recorded for both the native EPS and for the products generated by Smith degradation of the EPS, the following structure was determined for the repeat unit of the polysaccharide:[Figure presented] This is a novel D-galactan and represents the first structure for an EPS produced by a strain of Lactobacillus mucosae to be reported.",
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Isolation and characterization of a novel exopolysaccharide secreted by Lactobacillus mucosae VG1. / Fagunwa, Omololu; Ahmed, Hafiz; Sadiq, Sohaib; Humphreys, Paul; Mclay, Neil; Laws, Andrew.

In: Carbohydrate Research, Vol. 484, 107781, 01.10.2019.

Research output: Contribution to journalArticle

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AU - Ahmed, Hafiz

AU - Sadiq, Sohaib

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AU - Mclay, Neil

AU - Laws, Andrew

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