Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Gemma Monaghan; Douglas J. Clarke; Sue Povey; Chee Gee See; Maureen Boxer; Brian Burchell

doi:10.1006/geno.1994.1531

Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Gemma Monaghan, Douglas J. Clarke, Sue Povey, Chee Gee See, Maureen Boxer, Brian Burchell

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

Original language	English
Pages (from-to)	496-499
Number of pages	4
Journal	Genomics
Volume	23
Issue number	2
DOIs	https://doi.org/10.1006/geno.1994.1531
Publication status	Published - 15 Sep 1994
Externally published	Yes

Access to Document

10.1006/geno.1994.1531Licence: Unspecified

Cite this

@article{58a5a478c7914bf9a440cf14d28f1f10,

title = "Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13",

abstract = "Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.",

author = "Gemma Monaghan and Clarke, {Douglas J.} and Sue Povey and See, {Chee Gee} and Maureen Boxer and Brian Burchell",

year = "1994",

month = sep,

day = "15",

doi = "10.1006/geno.1994.1531",

language = "English",

volume = "23",

pages = "496--499",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

AU - Monaghan, Gemma

AU - Clarke, Douglas J.

AU - Povey, Sue

AU - See, Chee Gee

AU - Boxer, Maureen

AU - Burchell, Brian

PY - 1994/9/15

Y1 - 1994/9/15

N2 - Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

AB - Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

UR - http://www.scopus.com/inward/record.url?scp=0028136583&partnerID=8YFLogxK

U2 - 10.1006/geno.1994.1531

DO - 10.1006/geno.1994.1531

M3 - Article

AN - SCOPUS:0028136583

VL - 23

SP - 496

EP - 499

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -

Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Abstract

Access to Document

Other files and links

Fingerprint

Cite this