Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Gemma Monaghan, Douglas J. Clarke, Sue Povey, Chee Gee See, Maureen Boxer, Brian Burchell

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

LanguageEnglish
Pages496-499
Number of pages4
JournalGenomics
Volume23
Issue number2
DOIs
Publication statusPublished - 15 Sep 1994
Externally publishedYes

Fingerprint

Multigene Family
Chromosomes
Chromosomes, Human, Pair 4
Hybrid Cells
Genes
Yeast Artificial Chromosomes
Polymerase Chain Reaction
Glucuronosyltransferase
Pulsed Field Gel Electrophoresis
Bile Acids and Salts
Fluorescence In Situ Hybridization
Rodentia
Cell Line
DNA

Cite this

Monaghan, Gemma ; Clarke, Douglas J. ; Povey, Sue ; See, Chee Gee ; Boxer, Maureen ; Burchell, Brian. / Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13. In: Genomics. 1994 ; Vol. 23, No. 2. pp. 496-499.
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Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13. / Monaghan, Gemma; Clarke, Douglas J.; Povey, Sue; See, Chee Gee; Boxer, Maureen; Burchell, Brian.

In: Genomics, Vol. 23, No. 2, 15.09.1994, p. 496-499.

Research output: Contribution to journalArticle

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