Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Gemma Monaghan; Douglas J. Clarke; Sue Povey; Chee Gee See; Maureen Boxer; Brian Burchell

doi:10.1006/geno.1994.1531

Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Gemma Monaghan, Douglas J. Clarke, Sue Povey, Chee Gee See, Maureen Boxer, Brian Burchell

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Abstract

Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

Original language	English
Pages (from-to)	496-499
Number of pages	4
Journal	Genomics
Volume	23
Issue number	2
DOIs	https://doi.org/10.1006/geno.1994.1531
Publication status	Published - 15 Sept 1994
Externally published	Yes

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title = "Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13",

abstract = "Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.",

author = "Gemma Monaghan and Clarke, \{Douglas J.\} and Sue Povey and See, \{Chee Gee\} and Maureen Boxer and Brian Burchell",

year = "1994",

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doi = "10.1006/geno.1994.1531",

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T1 - Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

AU - Monaghan, Gemma

AU - Clarke, Douglas J.

AU - Povey, Sue

AU - See, Chee Gee

AU - Boxer, Maureen

AU - Burchell, Brian

PY - 1994/9/15

Y1 - 1994/9/15

N2 - Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

AB - Previously we mapped the gene encoding a human bile acid UDP- glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.

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U2 - 10.1006/geno.1994.1531

DO - 10.1006/geno.1994.1531

M3 - Article

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SN - 0888-7543

VL - 23

SP - 496

EP - 499

JO - Genomics

JF - Genomics

IS - 2

ER -

Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13

Abstract

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