Live Imaging of Planaria

Wei Shen, Yun Shen, Yun Wah Lam, Danny Chan

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)


Planarian regeneration involves a complex series of cellular events, precisely choreographed in space and time. Time-lapse imaging can provide powerful insights into tissue dynamics, as variously demonstrated in other model systems. However, time-lapse imaging of planarians has proven to be a challenge. Especially the requisite immobilization of the animals over extended periods of time is difficult, owing to their photophobic behavior and soft body architecture. Here, we describe a new embedding method using 2% (w/v) low melting agarose, and demonstrate that this method can effectively immobilize animals as long as 7 days. In combination with cell-permeable fluorescent dyes, this immobilization method allows for the time-lapse imaging of planaria during regeneration and other physiological processes.

Original languageEnglish
Title of host publicationPlanarian Regeneration
Subtitle of host publicationMethods and Protocols
EditorsJochen C. Rink
PublisherHumana Press Inc.
Number of pages12
ISBN (Electronic)9781493978021
ISBN (Print)9781493978007, 9781493992898
Publication statusPublished - 19 Jun 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
VolumeMIMB 1774
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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