Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions

Validation, protocols and computational models

Benita C. Percival, Martin Grootveld, Miles Gibson, Yasan Osman, Marco Molinari, Fereshteh Jafari, Tarsem Sahota, Mark Martin, Federico Casanova, Melissa L. Mather, Mark Edgar, Jinit Masania, Philippe B. Wilson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.

Original languageEnglish
Article number2
Number of pages33
JournalHigh-Throughput
Volume8
Issue number1
Early online date27 Dec 2018
DOIs
Publication statusPublished - 2019

Fingerprint

Point-of-Care Systems
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Magnetic resonance spectrometers
Metabolomics
Biopsy
Biomolecules
Acetone
Glucose
Urine
Technology
Costs and Cost Analysis
Liquids
Costs
Experiments

Cite this

Percival, Benita C. ; Grootveld, Martin ; Gibson, Miles ; Osman, Yasan ; Molinari, Marco ; Jafari, Fereshteh ; Sahota, Tarsem ; Martin, Mark ; Casanova, Federico ; Mather, Melissa L. ; Edgar, Mark ; Masania, Jinit ; Wilson, Philippe B. / Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions : Validation, protocols and computational models. In: High-Throughput. 2019 ; Vol. 8, No. 1.
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Percival, BC, Grootveld, M, Gibson, M, Osman, Y, Molinari, M, Jafari, F, Sahota, T, Martin, M, Casanova, F, Mather, ML, Edgar, M, Masania, J & Wilson, PB 2019, 'Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: Validation, protocols and computational models', High-Throughput, vol. 8, no. 1, 2. https://doi.org/10.3390/ht8010002

Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions : Validation, protocols and computational models. / Percival, Benita C.; Grootveld, Martin; Gibson, Miles; Osman, Yasan; Molinari, Marco; Jafari, Fereshteh; Sahota, Tarsem; Martin, Mark; Casanova, Federico; Mather, Melissa L.; Edgar, Mark; Masania, Jinit; Wilson, Philippe B.

In: High-Throughput, Vol. 8, No. 1, 2, 2019.

Research output: Contribution to journalArticle

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T1 - Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions

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AU - Percival, Benita C.

AU - Grootveld, Martin

AU - Gibson, Miles

AU - Osman, Yasan

AU - Molinari, Marco

AU - Jafari, Fereshteh

AU - Sahota, Tarsem

AU - Martin, Mark

AU - Casanova, Federico

AU - Mather, Melissa L.

AU - Edgar, Mark

AU - Masania, Jinit

AU - Wilson, Philippe B.

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