Macromolecular conformation of chitosan in dilute solution

A new global hydrodynamic approach

Gordon A. Morris, Jonathan Castile, Alan Smith, Gary G. Adams, Stephen E. Harding

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Chitosans of different molar masses were prepared by storing freshly prepared samples for up to 6 months at either 4, 25 or 40 °C. The weight-average molar masses, Mw and intrinsic viscosities, [η] were then measured using size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and a "rolling ball" viscometer, respectively. The solution conformation of chitosan was then estimated from:(a)the Mark-Houwink-Kuhn-Sakurada (MHKS) power law relationship [η] = kMw a and(b)the persistence length, Lp calculated from a new approach based on equivalent radii [Ortega, A., & Garcia de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475]. Both the MHKS power law exponent (a = 0.95 ± 0.01) and the persistence length (Lp = 16 ± 2 nm) are consistent with a semi-flexible rod type (or stiff coil) conformation for all 33 chitosans studied. A semi-flexible rod conformation was further supported by the Wales-van Holde ratio, the translational frictional ratio and sedimentation conformation zoning.

Original languageEnglish
Pages (from-to)616-621
Number of pages6
JournalCarbohydrate Polymers
Volume76
Issue number4
Early online date27 Nov 2008
DOIs
Publication statusPublished - 16 May 2009
Externally publishedYes

Fingerprint

Chitosan
Hydrodynamics
Conformations
Molar mass
Wales
Viscosity
Gel Chromatography
Lasers
Zoning
Size exclusion chromatography
Viscometers
Light
Weights and Measures
Sedimentation
Light scattering

Cite this

Morris, Gordon A. ; Castile, Jonathan ; Smith, Alan ; Adams, Gary G. ; Harding, Stephen E. / Macromolecular conformation of chitosan in dilute solution : A new global hydrodynamic approach. In: Carbohydrate Polymers. 2009 ; Vol. 76, No. 4. pp. 616-621.
@article{08a4b9b77a7b43c5bbe46380710d55a4,
title = "Macromolecular conformation of chitosan in dilute solution: A new global hydrodynamic approach",
abstract = "Chitosans of different molar masses were prepared by storing freshly prepared samples for up to 6 months at either 4, 25 or 40 °C. The weight-average molar masses, Mw and intrinsic viscosities, [η] were then measured using size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and a {"}rolling ball{"} viscometer, respectively. The solution conformation of chitosan was then estimated from:(a)the Mark-Houwink-Kuhn-Sakurada (MHKS) power law relationship [η] = kMw a and(b)the persistence length, Lp calculated from a new approach based on equivalent radii [Ortega, A., & Garcia de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475]. Both the MHKS power law exponent (a = 0.95 ± 0.01) and the persistence length (Lp = 16 ± 2 nm) are consistent with a semi-flexible rod type (or stiff coil) conformation for all 33 chitosans studied. A semi-flexible rod conformation was further supported by the Wales-van Holde ratio, the translational frictional ratio and sedimentation conformation zoning.",
keywords = "Chitosan, Equivalent radii, Intrinsic viscosity, Molar mass, Molar weight, Sedimentation coefficient, Semi-flexible rod conformation",
author = "Morris, {Gordon A.} and Jonathan Castile and Alan Smith and Adams, {Gary G.} and Harding, {Stephen E.}",
year = "2009",
month = "5",
day = "16",
doi = "10.1016/j.carbpol.2008.11.025",
language = "English",
volume = "76",
pages = "616--621",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",
number = "4",

}

Macromolecular conformation of chitosan in dilute solution : A new global hydrodynamic approach. / Morris, Gordon A.; Castile, Jonathan; Smith, Alan; Adams, Gary G.; Harding, Stephen E.

In: Carbohydrate Polymers, Vol. 76, No. 4, 16.05.2009, p. 616-621.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Macromolecular conformation of chitosan in dilute solution

T2 - A new global hydrodynamic approach

AU - Morris, Gordon A.

AU - Castile, Jonathan

AU - Smith, Alan

AU - Adams, Gary G.

AU - Harding, Stephen E.

PY - 2009/5/16

Y1 - 2009/5/16

N2 - Chitosans of different molar masses were prepared by storing freshly prepared samples for up to 6 months at either 4, 25 or 40 °C. The weight-average molar masses, Mw and intrinsic viscosities, [η] were then measured using size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and a "rolling ball" viscometer, respectively. The solution conformation of chitosan was then estimated from:(a)the Mark-Houwink-Kuhn-Sakurada (MHKS) power law relationship [η] = kMw a and(b)the persistence length, Lp calculated from a new approach based on equivalent radii [Ortega, A., & Garcia de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475]. Both the MHKS power law exponent (a = 0.95 ± 0.01) and the persistence length (Lp = 16 ± 2 nm) are consistent with a semi-flexible rod type (or stiff coil) conformation for all 33 chitosans studied. A semi-flexible rod conformation was further supported by the Wales-van Holde ratio, the translational frictional ratio and sedimentation conformation zoning.

AB - Chitosans of different molar masses were prepared by storing freshly prepared samples for up to 6 months at either 4, 25 or 40 °C. The weight-average molar masses, Mw and intrinsic viscosities, [η] were then measured using size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and a "rolling ball" viscometer, respectively. The solution conformation of chitosan was then estimated from:(a)the Mark-Houwink-Kuhn-Sakurada (MHKS) power law relationship [η] = kMw a and(b)the persistence length, Lp calculated from a new approach based on equivalent radii [Ortega, A., & Garcia de la Torre, J. (2007). Equivalent radii and ratios of radii from solution properties as indicators of macromolecular conformation, shape, and flexibility. Biomacromolecules, 8, 2464-2475]. Both the MHKS power law exponent (a = 0.95 ± 0.01) and the persistence length (Lp = 16 ± 2 nm) are consistent with a semi-flexible rod type (or stiff coil) conformation for all 33 chitosans studied. A semi-flexible rod conformation was further supported by the Wales-van Holde ratio, the translational frictional ratio and sedimentation conformation zoning.

KW - Chitosan

KW - Equivalent radii

KW - Intrinsic viscosity

KW - Molar mass

KW - Molar weight

KW - Sedimentation coefficient

KW - Semi-flexible rod conformation

UR - http://www.scopus.com/inward/record.url?scp=63149194922&partnerID=8YFLogxK

UR - https://www.journals.elsevier.com/carbohydrate-polymers/

U2 - 10.1016/j.carbpol.2008.11.025

DO - 10.1016/j.carbpol.2008.11.025

M3 - Article

VL - 76

SP - 616

EP - 621

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 4

ER -