MCAK associates with EB1

T. Lee, K. J. Langford, J. M. Askham, A. Brüning-Richardson, E. E. Morrison

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The microtubule (MT)-associated protein EB1 localizes to and promotes growth at MT plus ends. The MT depolymerizing kinesin MCAK has also been reported to track growing MT plus ends. Here, we confirm that human MCAK colocalizes with EB1 at growing MT ends when expressed as a GFP fusion protein in transfected cells. We show that MCAK associates with the C-terminus of EB1 and EB3 but much less efficiently with RP1. EB1 associates with the N-terminal localization and regulatory domain in MCAK but not with the motor domain of the protein. The interaction is competitive with the binding of other EB1 ligands and does not require MTs. Knockdown of EB1 expression using siRNA impaired the ability of GFP-MCAK to localize to MT tips in transfected cells. We propose that MCAK is targeted to growing MT ends by EB1, that MCAK is held in an inactive conformation when associated with EB1 and that this could provide the basis for a mechanism that facilitates rapid switching between phases of MT growth and depolymerization.

LanguageEnglish
Pages2494-2500
Number of pages7
JournalOncogene
Volume27
Issue number17
Early online date29 Oct 2007
DOIs
Publication statusPublished - 10 Apr 2008
Externally publishedYes

Fingerprint

Microtubules
Kinesin
Competitive Binding
Microtubule-Associated Proteins
Growth
Small Interfering RNA
Ligands
Proteins

Cite this

Lee, T., Langford, K. J., Askham, J. M., Brüning-Richardson, A., & Morrison, E. E. (2008). MCAK associates with EB1. Oncogene, 27(17), 2494-2500. https://doi.org/10.1038/sj.onc.1210867
Lee, T. ; Langford, K. J. ; Askham, J. M. ; Brüning-Richardson, A. ; Morrison, E. E. / MCAK associates with EB1. In: Oncogene. 2008 ; Vol. 27, No. 17. pp. 2494-2500.
@article{717457ee78114319af8f4c2143bdfd2a,
title = "MCAK associates with EB1",
abstract = "The microtubule (MT)-associated protein EB1 localizes to and promotes growth at MT plus ends. The MT depolymerizing kinesin MCAK has also been reported to track growing MT plus ends. Here, we confirm that human MCAK colocalizes with EB1 at growing MT ends when expressed as a GFP fusion protein in transfected cells. We show that MCAK associates with the C-terminus of EB1 and EB3 but much less efficiently with RP1. EB1 associates with the N-terminal localization and regulatory domain in MCAK but not with the motor domain of the protein. The interaction is competitive with the binding of other EB1 ligands and does not require MTs. Knockdown of EB1 expression using siRNA impaired the ability of GFP-MCAK to localize to MT tips in transfected cells. We propose that MCAK is targeted to growing MT ends by EB1, that MCAK is held in an inactive conformation when associated with EB1 and that this could provide the basis for a mechanism that facilitates rapid switching between phases of MT growth and depolymerization.",
keywords = "APC, EB1, MCAK, Microtubule",
author = "T. Lee and Langford, {K. J.} and Askham, {J. M.} and A. Br{\"u}ning-Richardson and Morrison, {E. E.}",
year = "2008",
month = "4",
day = "10",
doi = "10.1038/sj.onc.1210867",
language = "English",
volume = "27",
pages = "2494--2500",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "17",

}

Lee, T, Langford, KJ, Askham, JM, Brüning-Richardson, A & Morrison, EE 2008, 'MCAK associates with EB1', Oncogene, vol. 27, no. 17, pp. 2494-2500. https://doi.org/10.1038/sj.onc.1210867

MCAK associates with EB1. / Lee, T.; Langford, K. J.; Askham, J. M.; Brüning-Richardson, A.; Morrison, E. E.

In: Oncogene, Vol. 27, No. 17, 10.04.2008, p. 2494-2500.

Research output: Contribution to journalArticle

TY - JOUR

T1 - MCAK associates with EB1

AU - Lee, T.

AU - Langford, K. J.

AU - Askham, J. M.

AU - Brüning-Richardson, A.

AU - Morrison, E. E.

PY - 2008/4/10

Y1 - 2008/4/10

N2 - The microtubule (MT)-associated protein EB1 localizes to and promotes growth at MT plus ends. The MT depolymerizing kinesin MCAK has also been reported to track growing MT plus ends. Here, we confirm that human MCAK colocalizes with EB1 at growing MT ends when expressed as a GFP fusion protein in transfected cells. We show that MCAK associates with the C-terminus of EB1 and EB3 but much less efficiently with RP1. EB1 associates with the N-terminal localization and regulatory domain in MCAK but not with the motor domain of the protein. The interaction is competitive with the binding of other EB1 ligands and does not require MTs. Knockdown of EB1 expression using siRNA impaired the ability of GFP-MCAK to localize to MT tips in transfected cells. We propose that MCAK is targeted to growing MT ends by EB1, that MCAK is held in an inactive conformation when associated with EB1 and that this could provide the basis for a mechanism that facilitates rapid switching between phases of MT growth and depolymerization.

AB - The microtubule (MT)-associated protein EB1 localizes to and promotes growth at MT plus ends. The MT depolymerizing kinesin MCAK has also been reported to track growing MT plus ends. Here, we confirm that human MCAK colocalizes with EB1 at growing MT ends when expressed as a GFP fusion protein in transfected cells. We show that MCAK associates with the C-terminus of EB1 and EB3 but much less efficiently with RP1. EB1 associates with the N-terminal localization and regulatory domain in MCAK but not with the motor domain of the protein. The interaction is competitive with the binding of other EB1 ligands and does not require MTs. Knockdown of EB1 expression using siRNA impaired the ability of GFP-MCAK to localize to MT tips in transfected cells. We propose that MCAK is targeted to growing MT ends by EB1, that MCAK is held in an inactive conformation when associated with EB1 and that this could provide the basis for a mechanism that facilitates rapid switching between phases of MT growth and depolymerization.

KW - APC

KW - EB1

KW - MCAK

KW - Microtubule

UR - http://www.scopus.com/inward/record.url?scp=42049097980&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1210867

DO - 10.1038/sj.onc.1210867

M3 - Article

VL - 27

SP - 2494

EP - 2500

JO - Oncogene

T2 - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 17

ER -

Lee T, Langford KJ, Askham JM, Brüning-Richardson A, Morrison EE. MCAK associates with EB1. Oncogene. 2008 Apr 10;27(17):2494-2500. https://doi.org/10.1038/sj.onc.1210867