5 Citations (Scopus)

Abstract

Mesoscopic structure of pectin with different molecular characteristics was investigated by means of small angle X-ray scattering (SAXS), electrokinetic measurements and data modelling. The influence of a broad range of pH (2–7) on chain conformation in the dilute and semi-diluted regime was investigated. Scattering data and concomitant analysis revealed two length scales at all environmental conditions studied. pH showed greater influence at acidic values (pH 2.0) enhancing the globular component of the structure due to association of galacturonic acid residues. Double logarithmic scattering intensity plots revealed fractal dimensions of 1.9 ± 0.2 in the low-q regime and 1.5 ± 0.2 in the high q-region, irrespectively of the specific environment. Increase in branching of RG-I regions of the polysaccharide chains enhanced the compact conformation irrespectively of the pH or concentration. The present work shows that radical changes in pectin conformation can be induced only under strongly acidic conditions a finding that has important consequences in tailoring the technological performance of these biopolymers.
Original languageEnglish
Article numbere23016
Number of pages8
JournalBiopolymers
Volume107
Issue number6
Early online date25 Mar 2017
DOIs
Publication statusPublished - 1 Jun 2017

Fingerprint

Conformations
Scattering
Biopolymers
Fractal dimension
Polysaccharides
X ray scattering
Fractals
Data structures
Association reactions
Acids
X-Rays
pectin
galacturonic acid

Cite this

Alba, K. ; Bingham, R. J. ; Kontogiorgos, V. / Mesoscopic structure of pectin in solution. In: Biopolymers. 2017 ; Vol. 107, No. 6.
@article{668d7ac1c3a249a5a6a3b2d3914cd0f6,
title = "Mesoscopic structure of pectin in solution",
abstract = "Mesoscopic structure of pectin with different molecular characteristics was investigated by means of small angle X-ray scattering (SAXS), electrokinetic measurements and data modelling. The influence of a broad range of pH (2–7) on chain conformation in the dilute and semi-diluted regime was investigated. Scattering data and concomitant analysis revealed two length scales at all environmental conditions studied. pH showed greater influence at acidic values (pH 2.0) enhancing the globular component of the structure due to association of galacturonic acid residues. Double logarithmic scattering intensity plots revealed fractal dimensions of 1.9 ± 0.2 in the low-q regime and 1.5 ± 0.2 in the high q-region, irrespectively of the specific environment. Increase in branching of RG-I regions of the polysaccharide chains enhanced the compact conformation irrespectively of the pH or concentration. The present work shows that radical changes in pectin conformation can be induced only under strongly acidic conditions a finding that has important consequences in tailoring the technological performance of these biopolymers.",
keywords = "pectin, polyelectrolyte, polysaccharide, SAXS",
author = "K. Alba and Bingham, {R. J.} and V. Kontogiorgos",
year = "2017",
month = "6",
day = "1",
doi = "10.1002/bip.23016",
language = "English",
volume = "107",
journal = "Biopolymers",
issn = "0006-3525",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

Mesoscopic structure of pectin in solution. / Alba, K.; Bingham, R. J.; Kontogiorgos, V.

In: Biopolymers, Vol. 107, No. 6, e23016, 01.06.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mesoscopic structure of pectin in solution

AU - Alba, K.

AU - Bingham, R. J.

AU - Kontogiorgos, V.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Mesoscopic structure of pectin with different molecular characteristics was investigated by means of small angle X-ray scattering (SAXS), electrokinetic measurements and data modelling. The influence of a broad range of pH (2–7) on chain conformation in the dilute and semi-diluted regime was investigated. Scattering data and concomitant analysis revealed two length scales at all environmental conditions studied. pH showed greater influence at acidic values (pH 2.0) enhancing the globular component of the structure due to association of galacturonic acid residues. Double logarithmic scattering intensity plots revealed fractal dimensions of 1.9 ± 0.2 in the low-q regime and 1.5 ± 0.2 in the high q-region, irrespectively of the specific environment. Increase in branching of RG-I regions of the polysaccharide chains enhanced the compact conformation irrespectively of the pH or concentration. The present work shows that radical changes in pectin conformation can be induced only under strongly acidic conditions a finding that has important consequences in tailoring the technological performance of these biopolymers.

AB - Mesoscopic structure of pectin with different molecular characteristics was investigated by means of small angle X-ray scattering (SAXS), electrokinetic measurements and data modelling. The influence of a broad range of pH (2–7) on chain conformation in the dilute and semi-diluted regime was investigated. Scattering data and concomitant analysis revealed two length scales at all environmental conditions studied. pH showed greater influence at acidic values (pH 2.0) enhancing the globular component of the structure due to association of galacturonic acid residues. Double logarithmic scattering intensity plots revealed fractal dimensions of 1.9 ± 0.2 in the low-q regime and 1.5 ± 0.2 in the high q-region, irrespectively of the specific environment. Increase in branching of RG-I regions of the polysaccharide chains enhanced the compact conformation irrespectively of the pH or concentration. The present work shows that radical changes in pectin conformation can be induced only under strongly acidic conditions a finding that has important consequences in tailoring the technological performance of these biopolymers.

KW - pectin

KW - polyelectrolyte

KW - polysaccharide

KW - SAXS

U2 - 10.1002/bip.23016

DO - 10.1002/bip.23016

M3 - Article

VL - 107

JO - Biopolymers

JF - Biopolymers

SN - 0006-3525

IS - 6

M1 - e23016

ER -