Metal bioaccumulation and cellular fractionation in an epigeic earthworm (Lumbricus rubellus): The interactive influences of population exposure histories, site-specific geochemistry and mitochondrial genotype

Jane Andre, Stephen R. Stürzenbaum, Peter Kille, A. John Morgan, Mark E. Hodson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Subcellular fractionation techniques were used to describe temporal changes (at intervals from T0 to T70 days) in the Pb, Zn and P partitioning profiles of Lumbricus rubellus populations from one calcareous (MDH) and one acidic (MCS) geographically isolated Pb/Zn-mine sites and one reference site (CPF). MDH and MCS individuals were laboratory maintained on their native field soils; CPF worms were exposed to both MDH and MCS soils. Site-specific differences in metal partitioning were found: notably, the putatively metal-adapted populations, MDH and MCS, preferentially partitioned higher proportions of their accumulated tissue metal burdens into insoluble CaPO4-rich organelles compared with naive counterparts, CPF. Thus, it is plausible that efficient metal immobilization is a phenotypic trait characterising metal tolerant ecotypes. Mitochondrial cytochrome oxidase II (COII) genotyping revealed that the populations indigenous to mine and reference soils belong to distinct genetic lineages, differentiated by ∼13%, with 7 haplotypes within the reference site lineage but fewer (3 and 4, respectively) in the lineage common to the two mine sites. Collectively, these observations raise the possibility that site-related genotype differences could influence the toxico-availability of metals and, thus, represent a potential confounding variable in field-based eco-toxicological assessments.
LanguageEnglish
Pages1566-1573
Number of pages8
JournalSoil Biology and Biochemistry
Volume42
Issue number9
Early online date8 Jun 2010
DOIs
Publication statusPublished - Sep 2010
Externally publishedYes

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Lumbricus rubellus
Oligochaeta
geochemistry
bioaccumulation
at-risk population
earthworms
earthworm
fractionation
genotype
Metals
Genotype
metals
history
metal
Population
Soil
partitioning
Ecotype
soil
Confounding Factors (Epidemiology)

Cite this

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title = "Metal bioaccumulation and cellular fractionation in an epigeic earthworm (Lumbricus rubellus): The interactive influences of population exposure histories, site-specific geochemistry and mitochondrial genotype",
abstract = "Subcellular fractionation techniques were used to describe temporal changes (at intervals from T0 to T70 days) in the Pb, Zn and P partitioning profiles of Lumbricus rubellus populations from one calcareous (MDH) and one acidic (MCS) geographically isolated Pb/Zn-mine sites and one reference site (CPF). MDH and MCS individuals were laboratory maintained on their native field soils; CPF worms were exposed to both MDH and MCS soils. Site-specific differences in metal partitioning were found: notably, the putatively metal-adapted populations, MDH and MCS, preferentially partitioned higher proportions of their accumulated tissue metal burdens into insoluble CaPO4-rich organelles compared with naive counterparts, CPF. Thus, it is plausible that efficient metal immobilization is a phenotypic trait characterising metal tolerant ecotypes. Mitochondrial cytochrome oxidase II (COII) genotyping revealed that the populations indigenous to mine and reference soils belong to distinct genetic lineages, differentiated by ∼13{\%}, with 7 haplotypes within the reference site lineage but fewer (3 and 4, respectively) in the lineage common to the two mine sites. Collectively, these observations raise the possibility that site-related genotype differences could influence the toxico-availability of metals and, thus, represent a potential confounding variable in field-based eco-toxicological assessments.",
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Metal bioaccumulation and cellular fractionation in an epigeic earthworm (Lumbricus rubellus) : The interactive influences of population exposure histories, site-specific geochemistry and mitochondrial genotype. / Andre, Jane; Stürzenbaum, Stephen R.; Kille, Peter; Morgan, A. John; Hodson, Mark E.

In: Soil Biology and Biochemistry, Vol. 42, No. 9, 09.2010, p. 1566-1573.

Research output: Contribution to journalArticle

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T1 - Metal bioaccumulation and cellular fractionation in an epigeic earthworm (Lumbricus rubellus)

T2 - Soil Biology and Biochemistry

AU - Andre, Jane

AU - Stürzenbaum, Stephen R.

AU - Kille, Peter

AU - Morgan, A. John

AU - Hodson, Mark E.

PY - 2010/9

Y1 - 2010/9

N2 - Subcellular fractionation techniques were used to describe temporal changes (at intervals from T0 to T70 days) in the Pb, Zn and P partitioning profiles of Lumbricus rubellus populations from one calcareous (MDH) and one acidic (MCS) geographically isolated Pb/Zn-mine sites and one reference site (CPF). MDH and MCS individuals were laboratory maintained on their native field soils; CPF worms were exposed to both MDH and MCS soils. Site-specific differences in metal partitioning were found: notably, the putatively metal-adapted populations, MDH and MCS, preferentially partitioned higher proportions of their accumulated tissue metal burdens into insoluble CaPO4-rich organelles compared with naive counterparts, CPF. Thus, it is plausible that efficient metal immobilization is a phenotypic trait characterising metal tolerant ecotypes. Mitochondrial cytochrome oxidase II (COII) genotyping revealed that the populations indigenous to mine and reference soils belong to distinct genetic lineages, differentiated by ∼13%, with 7 haplotypes within the reference site lineage but fewer (3 and 4, respectively) in the lineage common to the two mine sites. Collectively, these observations raise the possibility that site-related genotype differences could influence the toxico-availability of metals and, thus, represent a potential confounding variable in field-based eco-toxicological assessments.

AB - Subcellular fractionation techniques were used to describe temporal changes (at intervals from T0 to T70 days) in the Pb, Zn and P partitioning profiles of Lumbricus rubellus populations from one calcareous (MDH) and one acidic (MCS) geographically isolated Pb/Zn-mine sites and one reference site (CPF). MDH and MCS individuals were laboratory maintained on their native field soils; CPF worms were exposed to both MDH and MCS soils. Site-specific differences in metal partitioning were found: notably, the putatively metal-adapted populations, MDH and MCS, preferentially partitioned higher proportions of their accumulated tissue metal burdens into insoluble CaPO4-rich organelles compared with naive counterparts, CPF. Thus, it is plausible that efficient metal immobilization is a phenotypic trait characterising metal tolerant ecotypes. Mitochondrial cytochrome oxidase II (COII) genotyping revealed that the populations indigenous to mine and reference soils belong to distinct genetic lineages, differentiated by ∼13%, with 7 haplotypes within the reference site lineage but fewer (3 and 4, respectively) in the lineage common to the two mine sites. Collectively, these observations raise the possibility that site-related genotype differences could influence the toxico-availability of metals and, thus, represent a potential confounding variable in field-based eco-toxicological assessments.

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KW - Field & lab exposures

KW - Genotyping

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